Experiment: McCas_formate_complete Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00275 and MMP_RS00280 are separated by 50 nucleotides MMP_RS00280 and MMP_RS00285 are separated by 4 nucleotides MMP_RS00285 and MMP_RS00290 are separated by 57 nucleotides MMP_RS00290 and MMP_RS00295 are separated by 38 nucleotides MMP_RS00295 and MMP_RS00300 are separated by 48 nucleotides
MMP_RS00275: MMP_RS00275 - polyprenyl synthetase family protein, at 62,928 to 63,881
_RS00275
MMP_RS00280: MMP_RS00280 - DUF2118 domain-containing protein, at 63,932 to 64,384
_RS00280
MMP_RS00285: MMP_RS00285 - TIGR04013 family B12-binding domain/radical SAM domain-containing protein, at 64,389 to 65,498
_RS00285
MMP_RS00290: MMP_RS00290 - hypothetical protein, at 65,556 to 65,828
_RS00290
MMP_RS00295: MMP_RS00295 - gamma carbonic anhydrase family protein, at 65,867 to 66,322
_RS00295
MMP_RS00300: MMP_RS00300 - 6 7-dimethyl-8-ribityllumazine synthase, at 66,371 to 66,784
_RS00300
Position (kb) 64
65
66 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 63.759 kb on - strand at 63.804 kb on - strand at 63.889 kb on - strand at 63.897 kb on - strand at 63.898 kb on + strand at 63.919 kb on - strand at 64.061 kb on - strand, within MMP_RS00280 at 64.071 kb on - strand, within MMP_RS00280 at 64.091 kb on - strand, within MMP_RS00280 at 64.128 kb on - strand, within MMP_RS00280 at 64.147 kb on - strand, within MMP_RS00280 at 64.154 kb on + strand, within MMP_RS00280 at 64.154 kb on + strand, within MMP_RS00280 at 64.162 kb on - strand, within MMP_RS00280 at 64.181 kb on + strand, within MMP_RS00280 at 64.203 kb on + strand, within MMP_RS00280 at 64.287 kb on - strand, within MMP_RS00280 at 64.292 kb on - strand, within MMP_RS00280 at 64.293 kb on + strand, within MMP_RS00280 at 64.293 kb on + strand, within MMP_RS00280 at 64.297 kb on - strand, within MMP_RS00280 at 64.309 kb on + strand, within MMP_RS00280 at 64.323 kb on - strand, within MMP_RS00280 at 64.328 kb on + strand, within MMP_RS00280 at 64.358 kb on - strand at 64.358 kb on - strand at 64.359 kb on + strand at 64.362 kb on - strand at 64.464 kb on + strand at 64.534 kb on - strand, within MMP_RS00285 at 64.535 kb on + strand, within MMP_RS00285 at 64.570 kb on - strand, within MMP_RS00285 at 64.632 kb on + strand, within MMP_RS00285 at 64.722 kb on - strand, within MMP_RS00285 at 64.766 kb on - strand, within MMP_RS00285 at 64.766 kb on - strand, within MMP_RS00285 at 64.777 kb on - strand, within MMP_RS00285 at 64.788 kb on - strand, within MMP_RS00285 at 64.824 kb on - strand, within MMP_RS00285 at 65.009 kb on - strand, within MMP_RS00285 at 65.009 kb on - strand, within MMP_RS00285 at 65.293 kb on + strand, within MMP_RS00285 at 65.371 kb on + strand, within MMP_RS00285 at 65.373 kb on - strand, within MMP_RS00285 at 65.437 kb on + strand at 65.464 kb on - strand at 65.464 kb on - strand at 65.465 kb on + strand at 65.498 kb on - strand at 65.499 kb on + strand at 65.508 kb on - strand at 65.521 kb on - strand at 65.522 kb on + strand at 65.522 kb on + strand at 65.522 kb on + strand at 65.523 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.577 kb on - strand at 65.577 kb on - strand at 65.578 kb on + strand at 65.578 kb on + strand at 65.578 kb on + strand at 65.587 kb on - strand, within MMP_RS00290 at 65.587 kb on - strand, within MMP_RS00290 at 65.619 kb on - strand, within MMP_RS00290 at 65.625 kb on - strand, within MMP_RS00290 at 65.664 kb on - strand, within MMP_RS00290 at 65.755 kb on - strand, within MMP_RS00290 at 65.773 kb on - strand, within MMP_RS00290 at 65.854 kb on - strand at 65.855 kb on + strand at 65.864 kb on + strand at 65.865 kb on - strand at 65.866 kb on + strand at 65.946 kb on - strand, within MMP_RS00295 at 66.252 kb on - strand, within MMP_RS00295 at 66.320 kb on - strand at 66.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Chloride 250 mM remove 63,759 - +1.3 63,804 - -2.1 63,889 - +0.3 63,897 - -0.6 63,898 + -1.2 63,919 - +0.6 64,061 - MMP_RS00280 0.28 +0.3 64,071 - MMP_RS00280 0.31 -4.2 64,091 - MMP_RS00280 0.35 -0.3 64,128 - MMP_RS00280 0.43 +0.4 64,147 - MMP_RS00280 0.47 +0.6 64,154 + MMP_RS00280 0.49 -1.8 64,154 + MMP_RS00280 0.49 +0.3 64,162 - MMP_RS00280 0.51 +0.9 64,181 + MMP_RS00280 0.55 +1.0 64,203 + MMP_RS00280 0.60 +0.1 64,287 - MMP_RS00280 0.78 +1.4 64,292 - MMP_RS00280 0.79 +0.1 64,293 + MMP_RS00280 0.80 +0.4 64,293 + MMP_RS00280 0.80 +0.6 64,297 - MMP_RS00280 0.81 +0.4 64,309 + MMP_RS00280 0.83 +0.4 64,323 - MMP_RS00280 0.86 +0.9 64,328 + MMP_RS00280 0.87 +0.1 64,358 - +0.7 64,358 - +0.6 64,359 + +0.5 64,362 - +1.1 64,464 + +1.2 64,534 - MMP_RS00285 0.13 -0.0 64,535 + MMP_RS00285 0.13 -0.8 64,570 - MMP_RS00285 0.16 +0.1 64,632 + MMP_RS00285 0.22 +1.6 64,722 - MMP_RS00285 0.30 +0.3 64,766 - MMP_RS00285 0.34 -1.1 64,766 - MMP_RS00285 0.34 -1.0 64,777 - MMP_RS00285 0.35 -1.3 64,788 - MMP_RS00285 0.36 +0.2 64,824 - MMP_RS00285 0.39 -0.5 65,009 - MMP_RS00285 0.56 -0.7 65,009 - MMP_RS00285 0.56 -1.0 65,293 + MMP_RS00285 0.81 +0.4 65,371 + MMP_RS00285 0.88 -0.2 65,373 - MMP_RS00285 0.89 -0.4 65,437 + +0.1 65,464 - -0.4 65,464 - +0.6 65,465 + +0.6 65,498 - -0.9 65,499 + -0.1 65,508 - +0.6 65,521 - +0.4 65,522 + -0.2 65,522 + +0.8 65,522 + -0.2 65,523 - -0.4 65,575 - +1.5 65,575 - +0.5 65,575 - +0.1 65,575 - +1.2 65,575 - +0.2 65,577 - +1.0 65,577 - +0.7 65,578 + -0.7 65,578 + -0.2 65,578 + -0.1 65,587 - MMP_RS00290 0.11 -1.7 65,587 - MMP_RS00290 0.11 +0.9 65,619 - MMP_RS00290 0.23 +0.0 65,625 - MMP_RS00290 0.25 +0.6 65,664 - MMP_RS00290 0.40 +0.0 65,755 - MMP_RS00290 0.73 -0.1 65,773 - MMP_RS00290 0.79 +2.0 65,854 - +0.9 65,855 + +1.3 65,864 + -0.5 65,865 - +1.4 65,866 + +1.0 65,946 - MMP_RS00295 0.17 +0.7 66,252 - MMP_RS00295 0.84 +1.2 66,320 - +1.6 66,320 - -3.1
Or see this region's nucleotide sequence