Experiment: McCas_formate_complete 2-bromoethanesulfonic 4.16666666666667E-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05570 and MMP_RS05575 are separated by 94 nucleotides MMP_RS05575 and MMP_RS05580 are separated by 3 nucleotides MMP_RS05580 and MMP_RS08890 are separated by 173 nucleotides
MMP_RS05570: MMP_RS05570 - phosphoglucosamine mutase, at 1,067,165 to 1,068,508
_RS05570
MMP_RS05575: MMP_RS05575 - hypothetical protein, at 1,068,603 to 1,069,547
_RS05575
MMP_RS05580: MMP_RS05580 - glycosyltransferase, at 1,069,551 to 1,070,360
_RS05580
MMP_RS08890: MMP_RS08890 - glycosyltransferase family 4 protein, at 1,070,534 to 1,071,628
_RS08890
Position (kb)
1068
1069
1070 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1068.127 kb on - strand at 1068.127 kb on - strand at 1068.155 kb on - strand at 1068.323 kb on + strand at 1068.326 kb on - strand at 1068.327 kb on + strand at 1068.327 kb on + strand at 1068.375 kb on + strand at 1068.541 kb on + strand at 1068.548 kb on - strand at 1068.549 kb on + strand at 1068.549 kb on + strand at 1068.549 kb on + strand at 1068.568 kb on - strand at 1068.569 kb on + strand at 1068.572 kb on - strand at 1068.573 kb on + strand at 1068.574 kb on - strand at 1068.574 kb on - strand at 1068.574 kb on - strand at 1068.574 kb on - strand at 1068.641 kb on - strand at 1068.676 kb on - strand at 1068.683 kb on - strand at 1068.687 kb on - strand at 1068.687 kb on - strand at 1068.791 kb on - strand, within MMP_RS05575 at 1068.827 kb on - strand, within MMP_RS05575 at 1068.851 kb on + strand, within MMP_RS05575 at 1069.044 kb on - strand, within MMP_RS05575 at 1069.045 kb on + strand, within MMP_RS05575 at 1069.071 kb on + strand, within MMP_RS05575 at 1069.071 kb on + strand, within MMP_RS05575 at 1069.076 kb on + strand, within MMP_RS05575 at 1069.084 kb on + strand, within MMP_RS05575 at 1069.088 kb on - strand, within MMP_RS05575 at 1069.091 kb on + strand, within MMP_RS05575 at 1069.091 kb on + strand, within MMP_RS05575 at 1069.095 kb on + strand, within MMP_RS05575 at 1069.105 kb on - strand, within MMP_RS05575 at 1069.120 kb on - strand, within MMP_RS05575 at 1069.261 kb on + strand, within MMP_RS05575 at 1069.323 kb on - strand, within MMP_RS05575 at 1069.324 kb on + strand, within MMP_RS05575 at 1069.351 kb on + strand, within MMP_RS05575 at 1069.352 kb on - strand, within MMP_RS05575 at 1069.352 kb on - strand, within MMP_RS05575 at 1069.355 kb on - strand, within MMP_RS05575 at 1069.356 kb on + strand, within MMP_RS05575 at 1069.415 kb on + strand, within MMP_RS05575 at 1069.444 kb on - strand, within MMP_RS05575 at 1069.450 kb on - strand, within MMP_RS05575 at 1069.451 kb on + strand, within MMP_RS05575 at 1069.468 kb on - strand at 1069.469 kb on + strand at 1069.469 kb on + strand at 1069.473 kb on - strand at 1069.474 kb on + strand at 1069.474 kb on + strand at 1069.478 kb on - strand at 1069.479 kb on + strand at 1069.496 kb on - strand at 1069.496 kb on - strand at 1069.496 kb on - strand at 1069.500 kb on - strand at 1069.501 kb on + strand at 1069.501 kb on + strand at 1069.546 kb on + strand at 1069.550 kb on - strand at 1069.550 kb on - strand at 1069.592 kb on - strand at 1069.666 kb on - strand, within MMP_RS05580 at 1069.683 kb on + strand, within MMP_RS05580 at 1069.704 kb on + strand, within MMP_RS05580 at 1069.705 kb on - strand, within MMP_RS05580 at 1069.713 kb on - strand, within MMP_RS05580 at 1069.739 kb on - strand, within MMP_RS05580 at 1069.742 kb on + strand, within MMP_RS05580 at 1069.763 kb on + strand, within MMP_RS05580 at 1069.780 kb on + strand, within MMP_RS05580 at 1069.780 kb on + strand, within MMP_RS05580 at 1069.780 kb on + strand, within MMP_RS05580 at 1069.780 kb on + strand, within MMP_RS05580 at 1069.781 kb on - strand, within MMP_RS05580 at 1069.781 kb on - strand, within MMP_RS05580 at 1069.782 kb on + strand, within MMP_RS05580 at 1069.782 kb on + strand, within MMP_RS05580 at 1069.782 kb on + strand, within MMP_RS05580 at 1069.782 kb on + strand, within MMP_RS05580 at 1069.784 kb on - strand, within MMP_RS05580 at 1069.784 kb on - strand, within MMP_RS05580 at 1069.784 kb on - strand, within MMP_RS05580 at 1069.784 kb on - strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.785 kb on + strand, within MMP_RS05580 at 1069.792 kb on - strand, within MMP_RS05580 at 1069.793 kb on + strand, within MMP_RS05580 at 1069.849 kb on - strand, within MMP_RS05580 at 1069.865 kb on + strand, within MMP_RS05580 at 1069.868 kb on - strand, within MMP_RS05580 at 1069.868 kb on - strand, within MMP_RS05580 at 1069.870 kb on - strand, within MMP_RS05580 at 1069.882 kb on + strand, within MMP_RS05580 at 1069.929 kb on - strand, within MMP_RS05580 at 1069.982 kb on - strand, within MMP_RS05580 at 1069.983 kb on + strand, within MMP_RS05580 at 1069.989 kb on + strand, within MMP_RS05580 at 1069.992 kb on + strand, within MMP_RS05580 at 1070.079 kb on - strand, within MMP_RS05580 at 1070.079 kb on - strand, within MMP_RS05580 at 1070.080 kb on + strand, within MMP_RS05580 at 1070.080 kb on + strand, within MMP_RS05580 at 1070.112 kb on + strand, within MMP_RS05580 at 1070.132 kb on - strand, within MMP_RS05580 at 1070.133 kb on + strand, within MMP_RS05580 at 1070.133 kb on + strand, within MMP_RS05580 at 1070.216 kb on + strand, within MMP_RS05580 at 1070.219 kb on - strand, within MMP_RS05580 at 1070.223 kb on + strand, within MMP_RS05580 at 1070.332 kb on - strand at 1070.333 kb on + strand at 1070.333 kb on + strand at 1070.335 kb on - strand at 1070.346 kb on - strand at 1070.347 kb on + strand at 1070.348 kb on - strand at 1070.348 kb on - strand at 1070.348 kb on - strand at 1070.359 kb on + strand at 1070.370 kb on - strand at 1070.373 kb on - strand at 1070.429 kb on - strand at 1070.438 kb on + strand at 1070.466 kb on - strand at 1070.470 kb on + strand at 1070.478 kb on - strand at 1070.479 kb on + strand at 1070.479 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 2-bromoethanesulfonic 4.16666666666667E-05 mM remove 1,068,127 - -0.2 1,068,127 - -0.7 1,068,155 - -1.7 1,068,323 + -1.6 1,068,326 - -0.9 1,068,327 + -0.3 1,068,327 + -1.9 1,068,375 + -0.9 1,068,541 + -3.6 1,068,548 - -2.8 1,068,549 + -2.9 1,068,549 + -2.1 1,068,549 + -1.4 1,068,568 - +1.4 1,068,569 + -1.6 1,068,572 - -1.3 1,068,573 + +0.6 1,068,574 - -1.8 1,068,574 - -1.9 1,068,574 - -1.6 1,068,574 - +1.1 1,068,641 - +1.2 1,068,676 - +1.8 1,068,683 - -1.3 1,068,687 - -0.7 1,068,687 - +1.2 1,068,791 - MMP_RS05575 0.20 -1.5 1,068,827 - MMP_RS05575 0.24 -0.4 1,068,851 + MMP_RS05575 0.26 +0.3 1,069,044 - MMP_RS05575 0.47 -0.2 1,069,045 + MMP_RS05575 0.47 +1.6 1,069,071 + MMP_RS05575 0.50 +1.4 1,069,071 + MMP_RS05575 0.50 -0.1 1,069,076 + MMP_RS05575 0.50 -2.3 1,069,084 + MMP_RS05575 0.51 -0.1 1,069,088 - MMP_RS05575 0.51 -0.1 1,069,091 + MMP_RS05575 0.52 +1.5 1,069,091 + MMP_RS05575 0.52 +2.6 1,069,095 + MMP_RS05575 0.52 -0.4 1,069,105 - MMP_RS05575 0.53 -0.0 1,069,120 - MMP_RS05575 0.55 -2.3 1,069,261 + MMP_RS05575 0.70 -1.3 1,069,323 - MMP_RS05575 0.76 -1.7 1,069,324 + MMP_RS05575 0.76 -0.9 1,069,351 + MMP_RS05575 0.79 -2.3 1,069,352 - MMP_RS05575 0.79 -1.5 1,069,352 - MMP_RS05575 0.79 +1.1 1,069,355 - MMP_RS05575 0.80 +0.5 1,069,356 + MMP_RS05575 0.80 +0.2 1,069,415 + MMP_RS05575 0.86 -0.5 1,069,444 - MMP_RS05575 0.89 -1.4 1,069,450 - MMP_RS05575 0.90 -2.4 1,069,451 + MMP_RS05575 0.90 +0.1 1,069,468 - +3.9 1,069,469 + +0.6 1,069,469 + -1.0 1,069,473 - +1.2 1,069,474 + +1.5 1,069,474 + +0.8 1,069,478 - +1.0 1,069,479 + -4.0 1,069,496 - +1.2 1,069,496 - +1.3 1,069,496 - +1.3 1,069,500 - +0.9 1,069,501 + -0.4 1,069,501 + -0.6 1,069,546 + -2.4 1,069,550 - -1.1 1,069,550 - -1.5 1,069,592 - -1.7 1,069,666 - MMP_RS05580 0.14 -1.6 1,069,683 + MMP_RS05580 0.16 +1.3 1,069,704 + MMP_RS05580 0.19 -2.1 1,069,705 - MMP_RS05580 0.19 +0.9 1,069,713 - MMP_RS05580 0.20 -1.1 1,069,739 - MMP_RS05580 0.23 -2.5 1,069,742 + MMP_RS05580 0.24 +0.6 1,069,763 + MMP_RS05580 0.26 -1.9 1,069,780 + MMP_RS05580 0.28 -0.1 1,069,780 + MMP_RS05580 0.28 +0.7 1,069,780 + MMP_RS05580 0.28 -2.0 1,069,780 + MMP_RS05580 0.28 -0.4 1,069,781 - MMP_RS05580 0.28 -0.5 1,069,781 - MMP_RS05580 0.28 -1.7 1,069,782 + MMP_RS05580 0.29 -1.3 1,069,782 + MMP_RS05580 0.29 -0.5 1,069,782 + MMP_RS05580 0.29 -0.6 1,069,782 + MMP_RS05580 0.29 -2.8 1,069,784 - MMP_RS05580 0.29 +1.0 1,069,784 - MMP_RS05580 0.29 +0.2 1,069,784 - MMP_RS05580 0.29 -0.5 1,069,784 - MMP_RS05580 0.29 -1.3 1,069,785 + MMP_RS05580 0.29 -0.5 1,069,785 + MMP_RS05580 0.29 -0.4 1,069,785 + MMP_RS05580 0.29 +0.9 1,069,785 + MMP_RS05580 0.29 +0.5 1,069,785 + MMP_RS05580 0.29 -2.0 1,069,792 - MMP_RS05580 0.30 -1.1 1,069,793 + MMP_RS05580 0.30 +3.4 1,069,849 - MMP_RS05580 0.37 -0.8 1,069,865 + MMP_RS05580 0.39 +1.3 1,069,868 - MMP_RS05580 0.39 -1.4 1,069,868 - MMP_RS05580 0.39 +1.0 1,069,870 - MMP_RS05580 0.39 -2.6 1,069,882 + MMP_RS05580 0.41 +1.0 1,069,929 - MMP_RS05580 0.47 +0.2 1,069,982 - MMP_RS05580 0.53 -2.3 1,069,983 + MMP_RS05580 0.53 -0.5 1,069,989 + MMP_RS05580 0.54 -1.4 1,069,992 + MMP_RS05580 0.54 -0.7 1,070,079 - MMP_RS05580 0.65 -2.3 1,070,079 - MMP_RS05580 0.65 -0.4 1,070,080 + MMP_RS05580 0.65 -1.1 1,070,080 + MMP_RS05580 0.65 -2.7 1,070,112 + MMP_RS05580 0.69 +0.7 1,070,132 - MMP_RS05580 0.72 -1.0 1,070,133 + MMP_RS05580 0.72 -3.6 1,070,133 + MMP_RS05580 0.72 -3.6 1,070,216 + MMP_RS05580 0.82 -1.2 1,070,219 - MMP_RS05580 0.82 +0.5 1,070,223 + MMP_RS05580 0.83 -1.4 1,070,332 - -2.9 1,070,333 + -0.7 1,070,333 + -0.2 1,070,335 - -1.9 1,070,346 - -1.0 1,070,347 + +0.4 1,070,348 - +1.1 1,070,348 - -0.6 1,070,348 - -0.7 1,070,359 + -0.1 1,070,370 - -1.9 1,070,373 - +1.0 1,070,429 - -0.8 1,070,438 + -2.1 1,070,466 - -0.3 1,070,470 + -1.1 1,070,478 - -0.3 1,070,479 + +0.1 1,070,479 + -3.4
Or see this region's nucleotide sequence