Experiment: McCas_formate_complete 2-bromoethanesulfonic 4.16666666666667E-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03525 and MMP_RS03530 are separated by 15 nucleotides MMP_RS03530 and MMP_RS03535 are separated by 241 nucleotides MMP_RS03535 and MMP_RS03540 are separated by 10 nucleotides MMP_RS03540 and MMP_RS03545 are separated by 136 nucleotides
MMP_RS03525: MMP_RS03525 - ketopantoate reductase family protein, at 660,406 to 661,326
_RS03525
MMP_RS03530: MMP_RS03530 - tetratricopeptide repeat protein, at 661,342 to 662,571
_RS03530
MMP_RS03535: MMP_RS03535 - helix-turn-helix transcriptional regulator, at 662,813 to 663,016
_RS03535
MMP_RS03540: MMP_RS03540 - DUF2178 domain-containing protein, at 663,027 to 663,413
_RS03540
MMP_RS03545: MMP_RS03545 - ATP-binding protein, at 663,550 to 664,947
_RS03545
Position (kb)
661
662
663 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 660.350 kb on - strand at 660.366 kb on - strand at 660.562 kb on - strand, within MMP_RS03525 at 660.562 kb on - strand, within MMP_RS03525 at 660.563 kb on + strand, within MMP_RS03525 at 660.564 kb on - strand, within MMP_RS03525 at 660.612 kb on - strand, within MMP_RS03525 at 660.616 kb on + strand, within MMP_RS03525 at 660.618 kb on + strand, within MMP_RS03525 at 660.735 kb on - strand, within MMP_RS03525 at 660.735 kb on - strand, within MMP_RS03525 at 660.762 kb on - strand, within MMP_RS03525 at 660.762 kb on - strand, within MMP_RS03525 at 660.797 kb on - strand, within MMP_RS03525 at 660.863 kb on - strand, within MMP_RS03525 at 660.875 kb on - strand, within MMP_RS03525 at 660.922 kb on + strand, within MMP_RS03525 at 660.952 kb on - strand, within MMP_RS03525 at 660.953 kb on + strand, within MMP_RS03525 at 660.966 kb on + strand, within MMP_RS03525 at 660.985 kb on + strand, within MMP_RS03525 at 661.042 kb on + strand, within MMP_RS03525 at 661.046 kb on - strand, within MMP_RS03525 at 661.049 kb on + strand, within MMP_RS03525 at 661.056 kb on + strand, within MMP_RS03525 at 661.089 kb on + strand, within MMP_RS03525 at 661.194 kb on - strand, within MMP_RS03525 at 661.367 kb on + strand at 661.382 kb on - strand at 661.382 kb on - strand at 661.407 kb on - strand at 661.549 kb on + strand, within MMP_RS03530 at 661.549 kb on + strand, within MMP_RS03530 at 661.556 kb on + strand, within MMP_RS03530 at 661.654 kb on + strand, within MMP_RS03530 at 661.724 kb on + strand, within MMP_RS03530 at 661.740 kb on + strand, within MMP_RS03530 at 661.770 kb on - strand, within MMP_RS03530 at 661.792 kb on + strand, within MMP_RS03530 at 661.842 kb on + strand, within MMP_RS03530 at 661.876 kb on + strand, within MMP_RS03530 at 661.877 kb on - strand, within MMP_RS03530 at 661.877 kb on - strand, within MMP_RS03530 at 661.877 kb on - strand, within MMP_RS03530 at 661.879 kb on - strand, within MMP_RS03530 at 661.879 kb on - strand, within MMP_RS03530 at 661.880 kb on + strand, within MMP_RS03530 at 661.880 kb on + strand, within MMP_RS03530 at 661.890 kb on + strand, within MMP_RS03530 at 661.892 kb on - strand, within MMP_RS03530 at 661.941 kb on - strand, within MMP_RS03530 at 661.942 kb on + strand, within MMP_RS03530 at 661.942 kb on + strand, within MMP_RS03530 at 661.944 kb on + strand, within MMP_RS03530 at 662.012 kb on + strand, within MMP_RS03530 at 662.064 kb on + strand, within MMP_RS03530 at 662.170 kb on - strand, within MMP_RS03530 at 662.420 kb on + strand, within MMP_RS03530 at 662.420 kb on + strand, within MMP_RS03530 at 662.468 kb on - strand at 662.496 kb on - strand at 662.497 kb on + strand at 662.499 kb on - strand at 662.527 kb on - strand at 662.558 kb on + strand at 662.573 kb on - strand at 662.592 kb on + strand at 662.600 kb on + strand at 662.629 kb on - strand at 662.802 kb on - strand at 662.803 kb on + strand at 662.824 kb on - strand at 662.833 kb on + strand at 662.840 kb on - strand, within MMP_RS03535 at 662.841 kb on + strand, within MMP_RS03535 at 662.841 kb on + strand, within MMP_RS03535 at 663.020 kb on - strand at 663.029 kb on + strand at 663.050 kb on - strand at 663.059 kb on + strand at 663.059 kb on + strand at 663.059 kb on + strand at 663.062 kb on - strand at 663.125 kb on - strand, within MMP_RS03540 at 663.272 kb on - strand, within MMP_RS03540 at 663.300 kb on - strand, within MMP_RS03540 at 663.302 kb on - strand, within MMP_RS03540 at 663.303 kb on + strand, within MMP_RS03540 at 663.306 kb on - strand, within MMP_RS03540 at 663.309 kb on - strand, within MMP_RS03540 at 663.310 kb on + strand, within MMP_RS03540 at 663.323 kb on + strand, within MMP_RS03540 at 663.370 kb on + strand, within MMP_RS03540 at 663.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 2-bromoethanesulfonic 4.16666666666667E-05 mM remove 660,350 - -1.3 660,366 - -1.6 660,562 - MMP_RS03525 0.17 +1.1 660,562 - MMP_RS03525 0.17 +0.3 660,563 + MMP_RS03525 0.17 +0.5 660,564 - MMP_RS03525 0.17 +0.7 660,612 - MMP_RS03525 0.22 +0.2 660,616 + MMP_RS03525 0.23 -1.0 660,618 + MMP_RS03525 0.23 -0.3 660,735 - MMP_RS03525 0.36 -1.1 660,735 - MMP_RS03525 0.36 +0.8 660,762 - MMP_RS03525 0.39 -0.5 660,762 - MMP_RS03525 0.39 -0.3 660,797 - MMP_RS03525 0.42 -1.6 660,863 - MMP_RS03525 0.50 -0.1 660,875 - MMP_RS03525 0.51 +1.5 660,922 + MMP_RS03525 0.56 -1.7 660,952 - MMP_RS03525 0.59 +2.3 660,953 + MMP_RS03525 0.59 +1.1 660,966 + MMP_RS03525 0.61 +0.8 660,985 + MMP_RS03525 0.63 -1.0 661,042 + MMP_RS03525 0.69 -0.0 661,046 - MMP_RS03525 0.69 -0.4 661,049 + MMP_RS03525 0.70 -1.4 661,056 + MMP_RS03525 0.71 +2.3 661,089 + MMP_RS03525 0.74 -1.2 661,194 - MMP_RS03525 0.86 -2.1 661,367 + +1.0 661,382 - -0.6 661,382 - +1.7 661,407 - +2.0 661,549 + MMP_RS03530 0.17 +1.4 661,549 + MMP_RS03530 0.17 +0.6 661,556 + MMP_RS03530 0.17 -0.1 661,654 + MMP_RS03530 0.25 -0.4 661,724 + MMP_RS03530 0.31 +0.7 661,740 + MMP_RS03530 0.32 -3.1 661,770 - MMP_RS03530 0.35 +1.4 661,792 + MMP_RS03530 0.37 +1.4 661,842 + MMP_RS03530 0.41 -0.7 661,876 + MMP_RS03530 0.43 +1.6 661,877 - MMP_RS03530 0.43 +0.4 661,877 - MMP_RS03530 0.43 +1.4 661,877 - MMP_RS03530 0.43 +0.0 661,879 - MMP_RS03530 0.44 -0.5 661,879 - MMP_RS03530 0.44 +0.6 661,880 + MMP_RS03530 0.44 -1.2 661,880 + MMP_RS03530 0.44 +1.5 661,890 + MMP_RS03530 0.45 +0.4 661,892 - MMP_RS03530 0.45 +1.1 661,941 - MMP_RS03530 0.49 +0.4 661,942 + MMP_RS03530 0.49 -2.8 661,942 + MMP_RS03530 0.49 +0.7 661,944 + MMP_RS03530 0.49 -1.9 662,012 + MMP_RS03530 0.54 -1.0 662,064 + MMP_RS03530 0.59 +1.0 662,170 - MMP_RS03530 0.67 +0.2 662,420 + MMP_RS03530 0.88 -2.5 662,420 + MMP_RS03530 0.88 -2.4 662,468 - +0.0 662,496 - +1.9 662,497 + -0.3 662,499 - -2.9 662,527 - +0.2 662,558 + -0.2 662,573 - +1.1 662,592 + +0.7 662,600 + -2.3 662,629 - -0.3 662,802 - -1.7 662,803 + +1.2 662,824 - -2.9 662,833 + +1.8 662,840 - MMP_RS03535 0.13 +1.0 662,841 + MMP_RS03535 0.14 -1.9 662,841 + MMP_RS03535 0.14 -0.9 663,020 - +0.7 663,029 + +2.7 663,050 - -2.1 663,059 + +0.8 663,059 + +0.9 663,059 + -2.3 663,062 - +1.0 663,125 - MMP_RS03540 0.25 +0.4 663,272 - MMP_RS03540 0.63 +1.5 663,300 - MMP_RS03540 0.71 +1.4 663,302 - MMP_RS03540 0.71 +1.9 663,303 + MMP_RS03540 0.71 +0.4 663,306 - MMP_RS03540 0.72 +2.2 663,309 - MMP_RS03540 0.73 -0.4 663,310 + MMP_RS03540 0.73 +0.8 663,323 + MMP_RS03540 0.76 +1.0 663,370 + MMP_RS03540 0.89 -3.7 663,523 - +0.5
Or see this region's nucleotide sequence