Experiment: McCas_formate_complete Spiramycin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03860 and MMP_RS03865 are separated by 937 nucleotides MMP_RS03865 and MMP_RS08965 are separated by 93 nucleotides MMP_RS08965 and MMP_RS03870 are separated by 20 nucleotides
MMP_RS03860: MMP_RS03860 - hypothetical protein, at 730,287 to 731,216
_RS03860
MMP_RS03865: MMP_RS03865 - aspartate/glutamate racemase family protein, at 732,154 to 732,858
_RS03865
MMP_RS08965: MMP_RS08965 - hypothetical protein, at 732,952 to 733,107
_RS08965
MMP_RS03870: MMP_RS03870 - purine NTPase, at 733,128 to 733,808
_RS03870
Position (kb)
732
733 Strain fitness (log2 ratio)
-2
-1
0
1 at 731.268 kb on - strand at 731.268 kb on - strand at 731.270 kb on - strand at 731.271 kb on + strand at 731.271 kb on + strand at 731.272 kb on - strand at 731.272 kb on - strand at 731.272 kb on - strand at 731.272 kb on - strand at 731.273 kb on + strand at 731.273 kb on + strand at 731.273 kb on + strand at 731.275 kb on - strand at 731.275 kb on - strand at 731.275 kb on - strand at 731.275 kb on - strand at 731.276 kb on + strand at 731.276 kb on + strand at 731.276 kb on + strand at 731.276 kb on + strand at 731.276 kb on + strand at 731.280 kb on + strand at 731.282 kb on - strand at 731.283 kb on + strand at 731.416 kb on - strand at 731.416 kb on - strand at 731.431 kb on - strand at 731.432 kb on + strand at 731.432 kb on + strand at 731.456 kb on + strand at 731.456 kb on + strand at 731.459 kb on + strand at 731.459 kb on + strand at 731.471 kb on - strand at 731.472 kb on + strand at 731.472 kb on + strand at 731.490 kb on + strand at 731.493 kb on - strand at 731.519 kb on - strand at 731.520 kb on + strand at 731.546 kb on - strand at 731.608 kb on - strand at 731.621 kb on - strand at 731.626 kb on + strand at 731.700 kb on + strand at 731.727 kb on - strand at 731.841 kb on - strand at 732.063 kb on - strand at 732.231 kb on - strand, within MMP_RS03865 at 732.232 kb on + strand, within MMP_RS03865 at 732.232 kb on + strand, within MMP_RS03865 at 732.249 kb on - strand, within MMP_RS03865 at 732.249 kb on - strand, within MMP_RS03865 at 732.281 kb on + strand, within MMP_RS03865 at 732.310 kb on + strand, within MMP_RS03865 at 732.310 kb on + strand, within MMP_RS03865 at 732.310 kb on + strand, within MMP_RS03865 at 732.373 kb on + strand, within MMP_RS03865 at 732.419 kb on + strand, within MMP_RS03865 at 732.419 kb on + strand, within MMP_RS03865 at 732.422 kb on + strand, within MMP_RS03865 at 732.491 kb on + strand, within MMP_RS03865 at 732.568 kb on - strand, within MMP_RS03865 at 732.570 kb on - strand, within MMP_RS03865 at 732.571 kb on + strand, within MMP_RS03865 at 732.616 kb on - strand, within MMP_RS03865 at 732.744 kb on - strand, within MMP_RS03865 at 732.775 kb on + strand, within MMP_RS03865 at 732.807 kb on + strand at 732.872 kb on - strand at 732.873 kb on + strand at 732.873 kb on + strand at 732.875 kb on - strand at 732.886 kb on + strand at 732.907 kb on + strand at 732.930 kb on + strand at 733.100 kb on + strand at 733.125 kb on - strand at 733.152 kb on - strand at 733.218 kb on - strand, within MMP_RS03870 at 733.547 kb on - strand, within MMP_RS03870 at 733.550 kb on + strand, within MMP_RS03870 at 733.566 kb on - strand, within MMP_RS03870 at 733.571 kb on - strand, within MMP_RS03870 at 733.622 kb on + strand, within MMP_RS03870 at 733.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Spiramycin 0.1 mM remove 731,268 - -1.7 731,268 - +0.2 731,270 - -0.4 731,271 + +1.5 731,271 + +0.5 731,272 - +0.6 731,272 - -0.1 731,272 - -0.6 731,272 - -0.3 731,273 + +0.1 731,273 + -0.0 731,273 + -0.2 731,275 - +0.1 731,275 - +0.3 731,275 - +0.4 731,275 - +0.5 731,276 + +0.1 731,276 + -0.1 731,276 + -1.4 731,276 + -0.4 731,276 + +0.4 731,280 + -0.2 731,282 - -0.1 731,283 + +0.2 731,416 - +0.2 731,416 - +0.9 731,431 - -1.9 731,432 + +0.0 731,432 + -1.4 731,456 + +0.4 731,456 + +0.2 731,459 + -0.0 731,459 + -0.3 731,471 - +1.0 731,472 + -0.4 731,472 + +1.3 731,490 + +0.0 731,493 - +0.9 731,519 - +0.1 731,520 + +0.2 731,546 - -0.2 731,608 - -0.5 731,621 - -0.3 731,626 + +0.2 731,700 + -0.9 731,727 - +1.3 731,841 - +0.1 732,063 - -1.0 732,231 - MMP_RS03865 0.11 -0.7 732,232 + MMP_RS03865 0.11 +0.4 732,232 + MMP_RS03865 0.11 +0.2 732,249 - MMP_RS03865 0.13 -0.6 732,249 - MMP_RS03865 0.13 +0.3 732,281 + MMP_RS03865 0.18 +0.6 732,310 + MMP_RS03865 0.22 -0.7 732,310 + MMP_RS03865 0.22 -0.7 732,310 + MMP_RS03865 0.22 -0.5 732,373 + MMP_RS03865 0.31 +0.1 732,419 + MMP_RS03865 0.38 -0.2 732,419 + MMP_RS03865 0.38 +0.3 732,422 + MMP_RS03865 0.38 +0.4 732,491 + MMP_RS03865 0.48 +0.4 732,568 - MMP_RS03865 0.59 +0.7 732,570 - MMP_RS03865 0.59 -0.2 732,571 + MMP_RS03865 0.59 +0.3 732,616 - MMP_RS03865 0.66 -0.8 732,744 - MMP_RS03865 0.84 -0.5 732,775 + MMP_RS03865 0.88 -0.3 732,807 + +1.0 732,872 - +0.4 732,873 + -0.1 732,873 + +0.5 732,875 - -0.2 732,886 + +0.3 732,907 + -0.3 732,930 + +0.6 733,100 + +0.1 733,125 - +1.0 733,152 - -1.3 733,218 - MMP_RS03870 0.13 -0.2 733,547 - MMP_RS03870 0.62 +1.5 733,550 + MMP_RS03870 0.62 +0.9 733,566 - MMP_RS03870 0.64 +1.1 733,571 - MMP_RS03870 0.65 -0.5 733,622 + MMP_RS03870 0.73 +0.9 733,855 + -0.6
Or see this region's nucleotide sequence