Experiment: McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04430 and MMP_RS04435 are separated by 27 nucleotides MMP_RS04435 and MMP_RS04440 are separated by 344 nucleotides MMP_RS04440 and MMP_RS04445 are separated by 42 nucleotides MMP_RS04445 and MMP_RS04450 are separated by 9 nucleotides MMP_RS04450 and MMP_RS04455 are separated by 49 nucleotides
MMP_RS04430: MMP_RS04430 - DUF1847 domain-containing protein, at 842,609 to 843,238
_RS04430
MMP_RS04435: MMP_RS04435 - pyridoxamine 5'-phosphate oxidase family protein, at 843,266 to 843,745
_RS04435
MMP_RS04440: MMP_RS04440 - nitrogenase iron protein, at 844,090 to 844,917
_RS04440
MMP_RS04445: MMP_RS04445 - P-II family nitrogen regulator, at 844,960 to 845,277
_RS04445
MMP_RS04450: MMP_RS04450 - P-II family nitrogen regulator, at 845,287 to 845,652
_RS04450
MMP_RS04455: MMP_RS04455 - nitrogenase subunit alpha, at 845,702 to 847,135
_RS04455
Position (kb)
844
845 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 843.094 kb on - strand, within MMP_RS04430 at 843.095 kb on + strand, within MMP_RS04430 at 843.129 kb on + strand, within MMP_RS04430 at 843.302 kb on - strand at 843.386 kb on - strand, within MMP_RS04435 at 843.387 kb on + strand, within MMP_RS04435 at 843.402 kb on + strand, within MMP_RS04435 at 843.416 kb on + strand, within MMP_RS04435 at 843.419 kb on - strand, within MMP_RS04435 at 843.425 kb on - strand, within MMP_RS04435 at 843.426 kb on + strand, within MMP_RS04435 at 843.505 kb on + strand, within MMP_RS04435 at 843.623 kb on - strand, within MMP_RS04435 at 843.623 kb on - strand, within MMP_RS04435 at 843.629 kb on - strand, within MMP_RS04435 at 843.629 kb on - strand, within MMP_RS04435 at 843.630 kb on + strand, within MMP_RS04435 at 843.645 kb on - strand, within MMP_RS04435 at 843.645 kb on - strand, within MMP_RS04435 at 843.651 kb on - strand, within MMP_RS04435 at 843.663 kb on + strand, within MMP_RS04435 at 843.682 kb on - strand, within MMP_RS04435 at 843.682 kb on - strand, within MMP_RS04435 at 843.682 kb on - strand, within MMP_RS04435 at 843.701 kb on - strand at 843.708 kb on - strand at 843.708 kb on - strand at 843.779 kb on + strand at 843.781 kb on - strand at 843.781 kb on - strand at 843.782 kb on + strand at 843.803 kb on - strand at 843.803 kb on - strand at 843.804 kb on + strand at 843.808 kb on - strand at 843.812 kb on - strand at 843.816 kb on + strand at 843.822 kb on + strand at 843.822 kb on + strand at 843.824 kb on - strand at 843.824 kb on - strand at 843.824 kb on - strand at 843.825 kb on + strand at 843.845 kb on + strand at 843.845 kb on + strand at 843.932 kb on + strand at 843.997 kb on - strand at 843.998 kb on + strand at 843.998 kb on + strand at 843.998 kb on + strand at 844.072 kb on - strand at 844.072 kb on - strand at 844.072 kb on - strand at 844.072 kb on - strand at 844.072 kb on - strand at 844.073 kb on + strand at 844.073 kb on + strand at 844.073 kb on + strand at 844.073 kb on + strand at 844.074 kb on - strand at 844.074 kb on - strand at 844.074 kb on - strand at 844.075 kb on + strand at 844.075 kb on + strand at 844.076 kb on - strand at 844.076 kb on - strand at 844.076 kb on - strand at 844.077 kb on + strand at 844.077 kb on + strand at 844.112 kb on + strand at 844.225 kb on + strand, within MMP_RS04440 at 844.256 kb on - strand, within MMP_RS04440 at 844.256 kb on - strand, within MMP_RS04440 at 844.257 kb on + strand, within MMP_RS04440 at 844.269 kb on - strand, within MMP_RS04440 at 844.269 kb on - strand, within MMP_RS04440 at 844.300 kb on + strand, within MMP_RS04440 at 844.337 kb on - strand, within MMP_RS04440 at 844.337 kb on - strand, within MMP_RS04440 at 844.481 kb on - strand, within MMP_RS04440 at 844.532 kb on + strand, within MMP_RS04440 at 844.655 kb on - strand, within MMP_RS04440 at 844.673 kb on - strand, within MMP_RS04440 at 844.808 kb on + strand, within MMP_RS04440 at 844.880 kb on - strand at 844.889 kb on - strand at 844.910 kb on - strand at 844.911 kb on + strand at 844.919 kb on - strand at 844.930 kb on + strand at 844.930 kb on + strand at 844.933 kb on - strand at 844.934 kb on + strand at 845.009 kb on - strand, within MMP_RS04445 at 845.086 kb on + strand, within MMP_RS04445 at 845.086 kb on + strand, within MMP_RS04445 at 845.105 kb on + strand, within MMP_RS04445 at 845.139 kb on + strand, within MMP_RS04445 at 845.165 kb on - strand, within MMP_RS04445 at 845.191 kb on - strand, within MMP_RS04445 at 845.191 kb on - strand, within MMP_RS04445 at 845.214 kb on - strand, within MMP_RS04445 at 845.245 kb on - strand, within MMP_RS04445 at 845.273 kb on - strand at 845.276 kb on + strand at 845.284 kb on - strand at 845.284 kb on - strand at 845.287 kb on + strand at 845.453 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.477 kb on + strand, within MMP_RS04450 at 845.501 kb on - strand, within MMP_RS04450 at 845.502 kb on + strand, within MMP_RS04450 at 845.502 kb on + strand, within MMP_RS04450 at 845.502 kb on + strand, within MMP_RS04450 at 845.531 kb on - strand, within MMP_RS04450 at 845.531 kb on - strand, within MMP_RS04450 at 845.545 kb on + strand, within MMP_RS04450 at 845.552 kb on - strand, within MMP_RS04450 at 845.553 kb on + strand, within MMP_RS04450 at 845.557 kb on + strand, within MMP_RS04450 at 845.592 kb on + strand, within MMP_RS04450 at 845.759 kb on - strand at 845.760 kb on + strand at 845.807 kb on + strand at 845.807 kb on + strand at 845.807 kb on + strand at 845.830 kb on - strand at 845.830 kb on - strand at 845.831 kb on + strand at 845.886 kb on - strand, within MMP_RS04455 at 845.895 kb on - strand, within MMP_RS04455 at 845.895 kb on - strand, within MMP_RS04455 at 845.896 kb on + strand, within MMP_RS04455 at 845.896 kb on + strand, within MMP_RS04455 at 845.905 kb on + strand, within MMP_RS04455 at 845.907 kb on - strand, within MMP_RS04455
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM remove 843,094 - MMP_RS04430 0.77 +0.1 843,095 + MMP_RS04430 0.77 -1.0 843,129 + MMP_RS04430 0.83 +0.4 843,302 - -0.0 843,386 - MMP_RS04435 0.25 -0.5 843,387 + MMP_RS04435 0.25 -2.4 843,402 + MMP_RS04435 0.28 -1.4 843,416 + MMP_RS04435 0.31 +0.5 843,419 - MMP_RS04435 0.32 -0.9 843,425 - MMP_RS04435 0.33 +0.7 843,426 + MMP_RS04435 0.33 -0.6 843,505 + MMP_RS04435 0.50 +0.5 843,623 - MMP_RS04435 0.74 +0.1 843,623 - MMP_RS04435 0.74 +0.4 843,629 - MMP_RS04435 0.76 -0.6 843,629 - MMP_RS04435 0.76 -0.9 843,630 + MMP_RS04435 0.76 -0.4 843,645 - MMP_RS04435 0.79 +0.5 843,645 - MMP_RS04435 0.79 +0.8 843,651 - MMP_RS04435 0.80 +0.6 843,663 + MMP_RS04435 0.83 +0.2 843,682 - MMP_RS04435 0.87 +1.1 843,682 - MMP_RS04435 0.87 +1.0 843,682 - MMP_RS04435 0.87 +0.5 843,701 - -0.6 843,708 - +1.4 843,708 - +0.2 843,779 + +0.6 843,781 - -1.4 843,781 - -0.5 843,782 + +0.2 843,803 - +1.5 843,803 - -0.7 843,804 + -1.3 843,808 - +0.3 843,812 - -0.2 843,816 + +1.4 843,822 + -1.0 843,822 + +0.7 843,824 - -0.2 843,824 - +1.0 843,824 - +0.3 843,825 + +1.8 843,845 + +1.1 843,845 + +1.1 843,932 + +0.5 843,997 - +1.4 843,998 + -0.8 843,998 + -1.4 843,998 + +0.3 844,072 - +1.7 844,072 - +0.8 844,072 - -1.4 844,072 - -3.9 844,072 - +1.0 844,073 + -1.4 844,073 + -1.0 844,073 + +1.6 844,073 + +1.7 844,074 - +0.2 844,074 - -3.8 844,074 - -0.3 844,075 + +0.9 844,075 + -0.8 844,076 - -0.4 844,076 - -0.0 844,076 - +0.8 844,077 + -0.0 844,077 + -0.2 844,112 + -1.5 844,225 + MMP_RS04440 0.16 +1.3 844,256 - MMP_RS04440 0.20 +1.5 844,256 - MMP_RS04440 0.20 +0.7 844,257 + MMP_RS04440 0.20 -0.5 844,269 - MMP_RS04440 0.22 -0.9 844,269 - MMP_RS04440 0.22 -0.2 844,300 + MMP_RS04440 0.25 +1.2 844,337 - MMP_RS04440 0.30 +1.0 844,337 - MMP_RS04440 0.30 +0.8 844,481 - MMP_RS04440 0.47 -2.4 844,532 + MMP_RS04440 0.53 +0.5 844,655 - MMP_RS04440 0.68 -0.1 844,673 - MMP_RS04440 0.70 +0.8 844,808 + MMP_RS04440 0.87 +0.3 844,880 - -1.9 844,889 - +0.3 844,910 - -0.2 844,911 + +1.1 844,919 - +0.6 844,930 + +1.6 844,930 + +1.7 844,933 - +1.6 844,934 + +0.0 845,009 - MMP_RS04445 0.15 -1.3 845,086 + MMP_RS04445 0.40 -0.2 845,086 + MMP_RS04445 0.40 +2.3 845,105 + MMP_RS04445 0.46 +0.8 845,139 + MMP_RS04445 0.56 -0.1 845,165 - MMP_RS04445 0.64 -0.8 845,191 - MMP_RS04445 0.73 +0.5 845,191 - MMP_RS04445 0.73 -1.4 845,214 - MMP_RS04445 0.80 -0.2 845,245 - MMP_RS04445 0.90 +0.5 845,273 - -0.5 845,276 + -2.2 845,284 - -0.2 845,284 - +0.7 845,287 + -0.2 845,453 - MMP_RS04450 0.45 +0.3 845,476 - MMP_RS04450 0.52 +0.6 845,476 - MMP_RS04450 0.52 +0.6 845,476 - MMP_RS04450 0.52 -1.5 845,476 - MMP_RS04450 0.52 -0.4 845,476 - MMP_RS04450 0.52 -1.4 845,477 + MMP_RS04450 0.52 -0.6 845,501 - MMP_RS04450 0.58 +1.3 845,502 + MMP_RS04450 0.59 -0.2 845,502 + MMP_RS04450 0.59 +1.8 845,502 + MMP_RS04450 0.59 +1.5 845,531 - MMP_RS04450 0.67 +1.3 845,531 - MMP_RS04450 0.67 +0.8 845,545 + MMP_RS04450 0.70 +0.1 845,552 - MMP_RS04450 0.72 -0.1 845,553 + MMP_RS04450 0.73 +2.0 845,557 + MMP_RS04450 0.74 +0.6 845,592 + MMP_RS04450 0.83 -1.0 845,759 - -0.3 845,760 + +0.5 845,807 + +0.7 845,807 + +1.2 845,807 + -3.9 845,830 - +1.2 845,830 - +1.6 845,831 + +1.1 845,886 - MMP_RS04455 0.13 -0.3 845,895 - MMP_RS04455 0.13 +0.3 845,895 - MMP_RS04455 0.13 +0.4 845,896 + MMP_RS04455 0.14 -0.1 845,896 + MMP_RS04455 0.14 +1.0 845,905 + MMP_RS04455 0.14 -2.0 845,907 - MMP_RS04455 0.14 +0.9
Or see this region's nucleotide sequence