Experiment: McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03435 and MMP_RS03440 are separated by 57 nucleotides MMP_RS03440 and MMP_RS03445 are separated by 75 nucleotides MMP_RS03445 and MMP_RS03450 are separated by 74 nucleotides
MMP_RS03435: MMP_RS03435 - ketol-acid reductoisomerase, at 645,729 to 646,721
_RS03435
MMP_RS03440: MMP_RS03440 - MoaD/ThiS family protein, at 646,779 to 646,973
_RS03440
MMP_RS03445: MMP_RS03445 - methanogenesis marker 17 protein, at 647,049 to 647,600
_RS03445
MMP_RS03450: MMP_RS03450 - glycine synthesis protein GlyXS <small>(from data)</small>, at 647,675 to 647,947
_RS03450
Position (kb) 646
647 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 645.917 kb on + strand, within MMP_RS03435 at 645.965 kb on + strand, within MMP_RS03435 at 645.965 kb on + strand, within MMP_RS03435 at 646.615 kb on - strand, within MMP_RS03435 at 646.660 kb on - strand at 646.720 kb on + strand at 646.870 kb on - strand, within MMP_RS03440 at 646.979 kb on - strand at 646.983 kb on - strand at 646.984 kb on + strand at 646.987 kb on - strand at 646.997 kb on + strand at 647.001 kb on - strand at 647.002 kb on + strand at 647.002 kb on + strand at 647.004 kb on + strand at 647.018 kb on - strand at 647.055 kb on - strand at 647.407 kb on - strand, within MMP_RS03445 at 647.695 kb on - strand at 647.797 kb on - strand, within MMP_RS03450 at 647.797 kb on - strand, within MMP_RS03450 at 647.852 kb on - strand, within MMP_RS03450 at 647.951 kb on - strand at 647.951 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM remove 645,917 + MMP_RS03435 0.19 +0.7 645,965 + MMP_RS03435 0.24 -1.1 645,965 + MMP_RS03435 0.24 +0.5 646,615 - MMP_RS03435 0.89 -2.3 646,660 - -0.9 646,720 + -0.0 646,870 - MMP_RS03440 0.47 -0.7 646,979 - -0.3 646,983 - +0.8 646,984 + +0.7 646,987 - +0.3 646,997 + -0.6 647,001 - -0.4 647,002 + +0.1 647,002 + +0.1 647,004 + +1.9 647,018 - -2.1 647,055 - -2.7 647,407 - MMP_RS03445 0.65 +0.3 647,695 - -3.9 647,797 - MMP_RS03450 0.45 +0.3 647,797 - MMP_RS03450 0.45 +0.9 647,852 - MMP_RS03450 0.65 -2.5 647,951 - -1.7 647,951 - -1.9
Or see this region's nucleotide sequence