Strain Fitness in Methanococcus maripaludis S2 around MMP_RS03210

Experiment: McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS03200 and MMP_RS03205 are separated by 136 nucleotidesMMP_RS03205 and MMP_RS03210 are separated by 147 nucleotidesMMP_RS03210 and MMP_RS03215 are separated by 52 nucleotidesMMP_RS03215 and MMP_RS03220 are separated by 1 nucleotides MMP_RS03200: MMP_RS03200 - methanogenesis marker 15 protein, at 603,102 to 604,337 _RS03200 MMP_RS03205: MMP_RS03205 - peptidyl-tRNA hydrolase Pth2, at 604,474 to 604,824 _RS03205 MMP_RS03210: MMP_RS03210 - tRNA guanosine(15) transglycosylase TgtA, at 604,972 to 606,921 _RS03210 MMP_RS03215: MMP_RS03215 - MBL fold metallo-hydrolase, at 606,974 to 607,516 _RS03215 MMP_RS03220: MMP_RS03220 - RNA 2' 3'-cyclic phosphodiesterase, at 607,518 to 608,042 _RS03220 Position (kb) 604 605 606 607Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 604.216 kb on - strandat 604.242 kb on + strandat 604.429 kb on - strandat 604.430 kb on + strandat 604.434 kb on - strandat 604.475 kb on - strandat 604.481 kb on + strandat 604.486 kb on - strandat 604.545 kb on - strand, within MMP_RS03205at 604.699 kb on + strand, within MMP_RS03205at 604.821 kb on + strandat 604.876 kb on + strandat 604.879 kb on + strandat 604.880 kb on - strandat 604.881 kb on + strandat 604.881 kb on + strandat 604.885 kb on + strandat 604.885 kb on + strandat 604.885 kb on + strandat 604.885 kb on + strandat 604.916 kb on - strandat 604.961 kb on + strandat 604.961 kb on + strandat 605.147 kb on - strandat 605.270 kb on - strand, within MMP_RS03210at 605.270 kb on - strand, within MMP_RS03210at 605.271 kb on + strand, within MMP_RS03210at 605.457 kb on - strand, within MMP_RS03210at 605.467 kb on + strand, within MMP_RS03210at 605.483 kb on + strand, within MMP_RS03210at 605.555 kb on - strand, within MMP_RS03210at 605.555 kb on - strand, within MMP_RS03210at 605.601 kb on - strand, within MMP_RS03210at 605.749 kb on - strand, within MMP_RS03210at 605.822 kb on - strand, within MMP_RS03210at 605.823 kb on + strand, within MMP_RS03210at 605.823 kb on + strand, within MMP_RS03210at 605.823 kb on + strand, within MMP_RS03210at 605.833 kb on - strand, within MMP_RS03210at 605.907 kb on - strand, within MMP_RS03210at 606.018 kb on - strand, within MMP_RS03210at 606.028 kb on - strand, within MMP_RS03210at 606.092 kb on + strand, within MMP_RS03210at 606.106 kb on + strand, within MMP_RS03210at 606.106 kb on + strand, within MMP_RS03210at 606.163 kb on - strand, within MMP_RS03210at 606.187 kb on + strand, within MMP_RS03210at 606.212 kb on - strand, within MMP_RS03210at 606.213 kb on + strand, within MMP_RS03210at 606.240 kb on + strand, within MMP_RS03210at 606.401 kb on - strand, within MMP_RS03210at 606.410 kb on + strand, within MMP_RS03210at 606.410 kb on + strand, within MMP_RS03210at 606.415 kb on - strand, within MMP_RS03210at 606.419 kb on + strand, within MMP_RS03210at 606.422 kb on - strand, within MMP_RS03210at 606.423 kb on + strand, within MMP_RS03210at 606.423 kb on + strand, within MMP_RS03210at 606.503 kb on + strand, within MMP_RS03210at 606.665 kb on - strand, within MMP_RS03210at 606.698 kb on - strand, within MMP_RS03210at 606.848 kb on - strandat 606.851 kb on - strandat 606.851 kb on - strandat 606.934 kb on + strandat 606.944 kb on - strandat 606.990 kb on + strandat 607.054 kb on - strand, within MMP_RS03215at 607.055 kb on + strand, within MMP_RS03215at 607.059 kb on + strand, within MMP_RS03215at 607.170 kb on + strand, within MMP_RS03215at 607.172 kb on + strand, within MMP_RS03215at 607.172 kb on + strand, within MMP_RS03215at 607.174 kb on - strand, within MMP_RS03215at 607.175 kb on + strand, within MMP_RS03215at 607.177 kb on + strand, within MMP_RS03215at 607.177 kb on + strand, within MMP_RS03215at 607.177 kb on + strand, within MMP_RS03215at 607.177 kb on + strand, within MMP_RS03215at 607.241 kb on + strand, within MMP_RS03215at 607.330 kb on + strand, within MMP_RS03215at 607.343 kb on - strand, within MMP_RS03215at 607.405 kb on - strand, within MMP_RS03215at 607.414 kb on + strand, within MMP_RS03215at 607.414 kb on + strand, within MMP_RS03215at 607.453 kb on + strand, within MMP_RS03215at 607.474 kb on + strandat 607.474 kb on + strandat 607.520 kb on + strandat 607.559 kb on - strandat 607.560 kb on + strandat 607.560 kb on + strandat 607.569 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM
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604,216 - -2.3
604,242 + -1.8
604,429 - +1.4
604,430 + +1.1
604,434 - +0.2
604,475 - +0.8
604,481 + -3.4
604,486 - -1.2
604,545 - MMP_RS03205 0.20 -2.8
604,699 + MMP_RS03205 0.64 -0.3
604,821 + -1.7
604,876 + +1.0
604,879 + -1.6
604,880 - -1.6
604,881 + +1.8
604,881 + -0.1
604,885 + -1.2
604,885 + -2.8
604,885 + -0.5
604,885 + -1.0
604,916 - -0.4
604,961 + +1.6
604,961 + -0.8
605,147 - +0.0
605,270 - MMP_RS03210 0.15 -2.4
605,270 - MMP_RS03210 0.15 -1.6
605,271 + MMP_RS03210 0.15 -1.6
605,457 - MMP_RS03210 0.25 +0.7
605,467 + MMP_RS03210 0.25 +0.7
605,483 + MMP_RS03210 0.26 -2.2
605,555 - MMP_RS03210 0.30 +1.0
605,555 - MMP_RS03210 0.30 -1.3
605,601 - MMP_RS03210 0.32 -1.1
605,749 - MMP_RS03210 0.40 +0.3
605,822 - MMP_RS03210 0.44 +0.4
605,823 + MMP_RS03210 0.44 -0.7
605,823 + MMP_RS03210 0.44 -1.8
605,823 + MMP_RS03210 0.44 -1.2
605,833 - MMP_RS03210 0.44 -0.8
605,907 - MMP_RS03210 0.48 -1.3
606,018 - MMP_RS03210 0.54 -1.0
606,028 - MMP_RS03210 0.54 -2.1
606,092 + MMP_RS03210 0.57 -0.5
606,106 + MMP_RS03210 0.58 -0.8
606,106 + MMP_RS03210 0.58 -1.4
606,163 - MMP_RS03210 0.61 -3.9
606,187 + MMP_RS03210 0.62 +0.3
606,212 - MMP_RS03210 0.64 -1.0
606,213 + MMP_RS03210 0.64 -0.8
606,240 + MMP_RS03210 0.65 -2.8
606,401 - MMP_RS03210 0.73 -1.0
606,410 + MMP_RS03210 0.74 -2.6
606,410 + MMP_RS03210 0.74 -0.7
606,415 - MMP_RS03210 0.74 -1.1
606,419 + MMP_RS03210 0.74 -0.3
606,422 - MMP_RS03210 0.74 -1.5
606,423 + MMP_RS03210 0.74 -2.6
606,423 + MMP_RS03210 0.74 -1.2
606,503 + MMP_RS03210 0.79 -0.8
606,665 - MMP_RS03210 0.87 -0.6
606,698 - MMP_RS03210 0.89 -1.4
606,848 - -1.0
606,851 - -1.0
606,851 - -0.6
606,934 + -2.2
606,944 - -0.2
606,990 + -2.1
607,054 - MMP_RS03215 0.15 -1.0
607,055 + MMP_RS03215 0.15 +0.1
607,059 + MMP_RS03215 0.16 +1.2
607,170 + MMP_RS03215 0.36 +0.5
607,172 + MMP_RS03215 0.36 -1.2
607,172 + MMP_RS03215 0.36 -0.6
607,174 - MMP_RS03215 0.37 -1.0
607,175 + MMP_RS03215 0.37 +1.1
607,177 + MMP_RS03215 0.37 -0.2
607,177 + MMP_RS03215 0.37 -0.7
607,177 + MMP_RS03215 0.37 +1.0
607,177 + MMP_RS03215 0.37 -1.3
607,241 + MMP_RS03215 0.49 +0.2
607,330 + MMP_RS03215 0.66 +0.4
607,343 - MMP_RS03215 0.68 +0.2
607,405 - MMP_RS03215 0.79 +1.5
607,414 + MMP_RS03215 0.81 -2.3
607,414 + MMP_RS03215 0.81 +2.2
607,453 + MMP_RS03215 0.88 -0.2
607,474 + +0.7
607,474 + +0.7
607,520 + -2.2
607,559 - +1.6
607,560 + -1.4
607,560 + +1.7
607,569 + +1.6

Or see this region's nucleotide sequence