Experiment: McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03075 and MMP_RS03080 are separated by 422 nucleotides MMP_RS03080 and MMP_RS03085 are separated by 4 nucleotides MMP_RS03085 and MMP_RS03090 are separated by 25 nucleotides
MMP_RS03075: MMP_RS03075 - valine--tRNA ligase, at 579,029 to 581,689
_RS03075
MMP_RS03080: MMP_RS03080 - universal stress protein, at 582,112 to 582,549
_RS03080
MMP_RS03085: MMP_RS03085 - endonuclease III, at 582,554 to 583,252
_RS03085
MMP_RS03090: MMP_RS03090 - cation:proton antiporter, at 583,278 to 584,447
_RS03090
Position (kb)
582
583
584 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 581.786 kb on - strand at 581.786 kb on - strand at 581.786 kb on - strand at 581.795 kb on - strand at 581.871 kb on + strand at 581.923 kb on + strand at 581.938 kb on - strand at 581.959 kb on + strand at 581.963 kb on + strand at 581.963 kb on + strand at 581.975 kb on - strand at 582.008 kb on - strand at 582.059 kb on + strand at 582.059 kb on + strand at 582.064 kb on - strand at 582.064 kb on - strand at 582.066 kb on - strand at 582.066 kb on - strand at 582.067 kb on + strand at 582.068 kb on - strand at 582.069 kb on + strand at 582.080 kb on - strand at 582.359 kb on - strand, within MMP_RS03080 at 582.360 kb on + strand, within MMP_RS03080 at 582.476 kb on - strand, within MMP_RS03080 at 582.477 kb on + strand, within MMP_RS03080 at 582.555 kb on - strand at 582.556 kb on + strand at 582.584 kb on - strand at 582.585 kb on + strand at 582.589 kb on + strand at 582.654 kb on + strand, within MMP_RS03085 at 582.737 kb on + strand, within MMP_RS03085 at 582.740 kb on - strand, within MMP_RS03085 at 582.740 kb on - strand, within MMP_RS03085 at 582.741 kb on + strand, within MMP_RS03085 at 582.755 kb on + strand, within MMP_RS03085 at 582.757 kb on + strand, within MMP_RS03085 at 582.869 kb on - strand, within MMP_RS03085 at 582.921 kb on - strand, within MMP_RS03085 at 582.938 kb on + strand, within MMP_RS03085 at 583.110 kb on - strand, within MMP_RS03085 at 583.365 kb on - strand at 583.396 kb on + strand, within MMP_RS03090 at 583.775 kb on - strand, within MMP_RS03090 at 583.786 kb on + strand, within MMP_RS03090 at 583.786 kb on + strand, within MMP_RS03090 at 583.792 kb on + strand, within MMP_RS03090 at 583.843 kb on + strand, within MMP_RS03090 at 583.845 kb on - strand, within MMP_RS03090 at 583.849 kb on + strand, within MMP_RS03090 at 583.889 kb on - strand, within MMP_RS03090 at 584.135 kb on + strand, within MMP_RS03090
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM remove 581,786 - +0.7 581,786 - +0.1 581,786 - +1.1 581,795 - -2.8 581,871 + -0.4 581,923 + -1.1 581,938 - -0.4 581,959 + +1.0 581,963 + -1.3 581,963 + -0.3 581,975 - +1.5 582,008 - +0.2 582,059 + +1.2 582,059 + -1.5 582,064 - +0.9 582,064 - -0.3 582,066 - -1.0 582,066 - +0.4 582,067 + +0.7 582,068 - +0.5 582,069 + +1.7 582,080 - -1.2 582,359 - MMP_RS03080 0.56 +2.0 582,360 + MMP_RS03080 0.57 -2.1 582,476 - MMP_RS03080 0.83 -3.8 582,477 + MMP_RS03080 0.83 -0.4 582,555 - +0.8 582,556 + +0.1 582,584 - +0.2 582,585 + -0.5 582,589 + -0.7 582,654 + MMP_RS03085 0.14 -2.0 582,737 + MMP_RS03085 0.26 +0.9 582,740 - MMP_RS03085 0.27 +0.8 582,740 - MMP_RS03085 0.27 -0.4 582,741 + MMP_RS03085 0.27 -1.1 582,755 + MMP_RS03085 0.29 -0.7 582,757 + MMP_RS03085 0.29 -0.5 582,869 - MMP_RS03085 0.45 +1.8 582,921 - MMP_RS03085 0.53 +2.4 582,938 + MMP_RS03085 0.55 +1.9 583,110 - MMP_RS03085 0.80 -2.1 583,365 - -1.5 583,396 + MMP_RS03090 0.10 -0.3 583,775 - MMP_RS03090 0.42 +0.2 583,786 + MMP_RS03090 0.43 -0.3 583,786 + MMP_RS03090 0.43 +0.5 583,792 + MMP_RS03090 0.44 -1.4 583,843 + MMP_RS03090 0.48 -0.4 583,845 - MMP_RS03090 0.48 +0.2 583,849 + MMP_RS03090 0.49 +0.5 583,889 - MMP_RS03090 0.52 +1.5 584,135 + MMP_RS03090 0.73 -0.2
Or see this region's nucleotide sequence