Experiment: McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02420 and MMP_RS02425 are separated by 119 nucleotides MMP_RS02425 and MMP_RS02430 are separated by 168 nucleotides MMP_RS02430 and MMP_RS02435 are separated by 71 nucleotides MMP_RS02435 and MMP_RS02440 are separated by 345 nucleotides
MMP_RS02420: MMP_RS02420 - DEAD/DEAH box helicase, at 450,214 to 451,830
_RS02420
MMP_RS02425: MMP_RS02425 - hypothetical protein, at 451,950 to 452,144
_RS02425
MMP_RS02430: MMP_RS02430 - hypothetical protein, at 452,313 to 453,959
_RS02430
MMP_RS02435: MMP_RS02435 - tRNA-Ser, at 454,031 to 454,115
_RS02435
MMP_RS02440: MMP_RS02440 - hypothetical protein, at 454,461 to 454,838
_RS02440
Position (kb) 452
453
454 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 451.335 kb on - strand at 451.336 kb on + strand at 451.338 kb on + strand at 451.496 kb on - strand at 451.602 kb on + strand at 451.614 kb on + strand at 451.707 kb on + strand at 451.832 kb on - strand at 451.958 kb on - strand at 451.988 kb on - strand, within MMP_RS02425 at 452.045 kb on - strand, within MMP_RS02425 at 452.049 kb on + strand, within MMP_RS02425 at 452.066 kb on - strand, within MMP_RS02425 at 452.082 kb on - strand, within MMP_RS02425 at 452.090 kb on - strand, within MMP_RS02425 at 452.107 kb on + strand, within MMP_RS02425 at 452.139 kb on - strand at 452.161 kb on - strand at 452.162 kb on + strand at 452.162 kb on + strand at 452.172 kb on - strand at 452.184 kb on - strand at 452.184 kb on - strand at 452.268 kb on + strand at 452.281 kb on - strand at 452.282 kb on + strand at 452.322 kb on - strand at 452.322 kb on - strand at 452.372 kb on - strand at 452.401 kb on - strand at 452.469 kb on + strand at 452.469 kb on + strand at 452.469 kb on + strand at 452.477 kb on - strand at 452.478 kb on + strand, within MMP_RS02430 at 452.481 kb on - strand, within MMP_RS02430 at 452.515 kb on - strand, within MMP_RS02430 at 452.558 kb on - strand, within MMP_RS02430 at 452.560 kb on - strand, within MMP_RS02430 at 452.601 kb on + strand, within MMP_RS02430 at 452.760 kb on + strand, within MMP_RS02430 at 452.791 kb on - strand, within MMP_RS02430 at 452.792 kb on + strand, within MMP_RS02430 at 452.862 kb on - strand, within MMP_RS02430 at 453.079 kb on + strand, within MMP_RS02430 at 453.313 kb on + strand, within MMP_RS02430 at 453.313 kb on + strand, within MMP_RS02430 at 453.451 kb on - strand, within MMP_RS02430 at 453.452 kb on + strand, within MMP_RS02430 at 453.459 kb on + strand, within MMP_RS02430 at 453.459 kb on + strand, within MMP_RS02430 at 453.463 kb on - strand, within MMP_RS02430 at 453.633 kb on + strand, within MMP_RS02430 at 453.670 kb on - strand, within MMP_RS02430 at 453.671 kb on + strand, within MMP_RS02430 at 453.676 kb on - strand, within MMP_RS02430 at 453.677 kb on + strand, within MMP_RS02430 at 453.693 kb on - strand, within MMP_RS02430 at 453.693 kb on - strand, within MMP_RS02430 at 453.722 kb on - strand, within MMP_RS02430 at 453.734 kb on - strand, within MMP_RS02430 at 453.735 kb on + strand, within MMP_RS02430 at 453.745 kb on - strand, within MMP_RS02430 at 453.858 kb on + strand at 453.928 kb on - strand at 453.928 kb on - strand at 453.928 kb on - strand at 453.931 kb on - strand at 453.965 kb on + strand at 453.970 kb on - strand at 453.971 kb on + strand at 454.170 kb on - strand at 454.185 kb on - strand at 454.196 kb on - strand at 454.312 kb on + strand at 454.312 kb on + strand at 454.314 kb on - strand at 454.315 kb on + strand at 454.504 kb on + strand, within MMP_RS02440 at 454.506 kb on + strand, within MMP_RS02440 at 454.506 kb on + strand, within MMP_RS02440 at 454.515 kb on - strand, within MMP_RS02440 at 454.516 kb on + strand, within MMP_RS02440 at 454.573 kb on + strand, within MMP_RS02440 at 454.624 kb on - strand, within MMP_RS02440 at 454.624 kb on - strand, within MMP_RS02440 at 454.841 kb on + strand at 454.862 kb on - strand at 454.944 kb on - strand at 454.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM remove 451,335 - -1.9 451,336 + +0.1 451,338 + -1.0 451,496 - +0.0 451,602 + -0.2 451,614 + -1.8 451,707 + +0.2 451,832 - +0.3 451,958 - +0.3 451,988 - MMP_RS02425 0.19 +0.4 452,045 - MMP_RS02425 0.49 +1.1 452,049 + MMP_RS02425 0.51 -2.8 452,066 - MMP_RS02425 0.59 +1.2 452,082 - MMP_RS02425 0.68 +0.4 452,090 - MMP_RS02425 0.72 -1.9 452,107 + MMP_RS02425 0.81 -0.1 452,139 - -4.4 452,161 - -0.6 452,162 + +0.5 452,162 + +3.5 452,172 - +0.7 452,184 - -0.6 452,184 - -1.7 452,268 + -1.1 452,281 - +2.3 452,282 + +1.1 452,322 - +0.1 452,322 - +2.0 452,372 - -0.1 452,401 - -0.6 452,469 + +0.9 452,469 + -1.2 452,469 + -0.2 452,477 - +1.6 452,478 + MMP_RS02430 0.10 -0.4 452,481 - MMP_RS02430 0.10 +0.4 452,515 - MMP_RS02430 0.12 +1.4 452,558 - MMP_RS02430 0.15 -0.1 452,560 - MMP_RS02430 0.15 +0.7 452,601 + MMP_RS02430 0.17 +2.1 452,760 + MMP_RS02430 0.27 -1.2 452,791 - MMP_RS02430 0.29 +1.0 452,792 + MMP_RS02430 0.29 +1.2 452,862 - MMP_RS02430 0.33 -0.7 453,079 + MMP_RS02430 0.47 +1.3 453,313 + MMP_RS02430 0.61 -0.3 453,313 + MMP_RS02430 0.61 -0.1 453,451 - MMP_RS02430 0.69 -0.7 453,452 + MMP_RS02430 0.69 +0.2 453,459 + MMP_RS02430 0.70 +1.8 453,459 + MMP_RS02430 0.70 -0.7 453,463 - MMP_RS02430 0.70 -0.1 453,633 + MMP_RS02430 0.80 +1.6 453,670 - MMP_RS02430 0.82 +0.8 453,671 + MMP_RS02430 0.82 +0.0 453,676 - MMP_RS02430 0.83 +0.3 453,677 + MMP_RS02430 0.83 +0.8 453,693 - MMP_RS02430 0.84 +1.6 453,693 - MMP_RS02430 0.84 +0.5 453,722 - MMP_RS02430 0.86 -0.5 453,734 - MMP_RS02430 0.86 +2.5 453,735 + MMP_RS02430 0.86 +0.4 453,745 - MMP_RS02430 0.87 +1.7 453,858 + -1.2 453,928 - +0.4 453,928 - -1.0 453,928 - +0.3 453,931 - +0.2 453,965 + +1.3 453,970 - +0.0 453,971 + +1.1 454,170 - +0.2 454,185 - -0.7 454,196 - +0.7 454,312 + +0.6 454,312 + +0.9 454,314 - +1.5 454,315 + +0.3 454,504 + MMP_RS02440 0.11 +0.8 454,506 + MMP_RS02440 0.12 +0.5 454,506 + MMP_RS02440 0.12 +0.9 454,515 - MMP_RS02440 0.14 -1.2 454,516 + MMP_RS02440 0.15 +2.3 454,573 + MMP_RS02440 0.30 +2.6 454,624 - MMP_RS02440 0.43 -2.8 454,624 - MMP_RS02440 0.43 +1.2 454,841 + +1.1 454,862 - +1.6 454,944 - -0.5 454,944 - +0.8
Or see this region's nucleotide sequence