Experiment: McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02015 and MMP_RS02020 are separated by 5 nucleotides MMP_RS02020 and MMP_RS02025 are separated by 4 nucleotides MMP_RS02025 and MMP_RS02030 are separated by 179 nucleotides MMP_RS02030 and MMP_RS02035 are separated by 78 nucleotides
MMP_RS02015: MMP_RS02015 - amidohydrolase, at 374,873 to 376,156
_RS02015
MMP_RS02020: MMP_RS02020 - gamma-glutamylcyclotransferase, at 376,162 to 376,485
_RS02020
MMP_RS02025: MMP_RS02025 - DNA-directed DNA polymerase, at 376,490 to 378,844
_RS02025
MMP_RS02030: MMP_RS02030 - homoaconitase small subunit, at 379,024 to 379,512
_RS02030
MMP_RS02035: MMP_RS02035 - ribosome biogenesis/translation initiation ATPase RLI, at 379,591 to 381,363
_RS02035
Position (kb) 376
377
378
379 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 375.518 kb on - strand, within MMP_RS02015 at 375.626 kb on + strand, within MMP_RS02015 at 375.665 kb on - strand, within MMP_RS02015 at 375.667 kb on - strand, within MMP_RS02015 at 375.835 kb on + strand, within MMP_RS02015 at 376.049 kb on + strand at 376.127 kb on + strand at 376.131 kb on - strand at 376.132 kb on + strand at 376.179 kb on + strand at 376.221 kb on - strand, within MMP_RS02020 at 376.300 kb on - strand, within MMP_RS02020 at 376.318 kb on + strand, within MMP_RS02020 at 376.385 kb on - strand, within MMP_RS02020 at 376.479 kb on + strand at 376.568 kb on - strand at 376.679 kb on - strand at 376.682 kb on + strand at 376.682 kb on + strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.730 kb on - strand, within MMP_RS02025 at 376.768 kb on - strand, within MMP_RS02025 at 376.789 kb on - strand, within MMP_RS02025 at 376.974 kb on + strand, within MMP_RS02025 at 377.239 kb on + strand, within MMP_RS02025 at 377.542 kb on - strand, within MMP_RS02025 at 377.543 kb on + strand, within MMP_RS02025 at 377.550 kb on + strand, within MMP_RS02025 at 377.564 kb on - strand, within MMP_RS02025 at 377.582 kb on + strand, within MMP_RS02025 at 377.651 kb on - strand, within MMP_RS02025 at 377.810 kb on - strand, within MMP_RS02025 at 377.869 kb on + strand, within MMP_RS02025 at 377.872 kb on + strand, within MMP_RS02025 at 377.909 kb on - strand, within MMP_RS02025 at 378.014 kb on - strand, within MMP_RS02025 at 378.064 kb on + strand, within MMP_RS02025 at 378.261 kb on - strand, within MMP_RS02025 at 378.418 kb on - strand, within MMP_RS02025 at 378.473 kb on + strand, within MMP_RS02025 at 378.506 kb on + strand, within MMP_RS02025 at 378.540 kb on + strand, within MMP_RS02025 at 378.770 kb on - strand at 378.771 kb on + strand at 378.771 kb on + strand at 378.782 kb on - strand at 378.791 kb on - strand at 378.791 kb on - strand at 378.791 kb on - strand at 378.792 kb on + strand at 378.792 kb on + strand at 378.792 kb on + strand at 378.795 kb on - strand at 378.796 kb on + strand at 378.796 kb on + strand at 378.925 kb on - strand at 378.964 kb on - strand at 379.190 kb on - strand at 379.380 kb on + strand at 379.550 kb on + strand at 379.557 kb on + strand at 379.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM remove 375,518 - MMP_RS02015 0.50 -0.7 375,626 + MMP_RS02015 0.59 -0.1 375,665 - MMP_RS02015 0.62 -0.8 375,667 - MMP_RS02015 0.62 +1.7 375,835 + MMP_RS02015 0.75 +0.5 376,049 + +0.6 376,127 + +1.1 376,131 - -2.0 376,132 + -1.1 376,179 + +1.3 376,221 - MMP_RS02020 0.18 -1.8 376,300 - MMP_RS02020 0.43 -0.4 376,318 + MMP_RS02020 0.48 -1.4 376,385 - MMP_RS02020 0.69 -1.3 376,479 + +1.7 376,568 - -1.5 376,679 - -0.9 376,682 + +0.1 376,682 + -0.6 376,691 - -2.6 376,691 - -2.3 376,691 - -2.7 376,730 - MMP_RS02025 0.10 -1.5 376,768 - MMP_RS02025 0.12 +0.2 376,789 - MMP_RS02025 0.13 -1.2 376,974 + MMP_RS02025 0.21 +0.3 377,239 + MMP_RS02025 0.32 -1.8 377,542 - MMP_RS02025 0.45 +0.2 377,543 + MMP_RS02025 0.45 +1.0 377,550 + MMP_RS02025 0.45 +0.0 377,564 - MMP_RS02025 0.46 -2.3 377,582 + MMP_RS02025 0.46 +1.6 377,651 - MMP_RS02025 0.49 -1.1 377,810 - MMP_RS02025 0.56 -0.0 377,869 + MMP_RS02025 0.59 -1.2 377,872 + MMP_RS02025 0.59 +0.8 377,909 - MMP_RS02025 0.60 -1.1 378,014 - MMP_RS02025 0.65 +1.0 378,064 + MMP_RS02025 0.67 -0.0 378,261 - MMP_RS02025 0.75 -1.6 378,418 - MMP_RS02025 0.82 +0.1 378,473 + MMP_RS02025 0.84 -0.7 378,506 + MMP_RS02025 0.86 -1.8 378,540 + MMP_RS02025 0.87 -1.5 378,770 - -0.0 378,771 + +2.4 378,771 + -1.8 378,782 - +1.8 378,791 - -1.9 378,791 - +0.1 378,791 - +0.0 378,792 + -3.2 378,792 + -2.4 378,792 + -3.7 378,795 - -0.8 378,796 + +0.1 378,796 + -1.7 378,925 - -2.1 378,964 - -1.4 379,190 - -2.0 379,380 + -1.6 379,550 + -0.4 379,557 + -0.0 379,615 - -1.4
Or see this region's nucleotide sequence