Experiment: McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00845 and MMP_RS00850 are separated by 33 nucleotides MMP_RS00850 and MMP_RS00855 are separated by 69 nucleotides MMP_RS00855 and MMP_RS00860 are separated by 4 nucleotides
MMP_RS00845: MMP_RS00845 - acetate--CoA ligase, at 158,021 to 159,931
_RS00845
MMP_RS00850: MMP_RS00850 - TIGR01177 family methyltransferase, at 159,965 to 161,017
_RS00850
MMP_RS00855: MMP_RS00855 - DUF367 family protein, at 161,087 to 161,602
_RS00855
MMP_RS00860: MMP_RS00860 - 50S ribosomal protein L40e, at 161,607 to 161,750
_RS00860
Position (kb) 159
160
161
162 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 159.061 kb on - strand, within MMP_RS00845 at 159.099 kb on - strand, within MMP_RS00845 at 159.165 kb on + strand, within MMP_RS00845 at 159.172 kb on + strand, within MMP_RS00845 at 159.232 kb on - strand, within MMP_RS00845 at 159.233 kb on + strand, within MMP_RS00845 at 159.235 kb on - strand, within MMP_RS00845 at 159.235 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.237 kb on - strand, within MMP_RS00845 at 159.238 kb on + strand, within MMP_RS00845 at 159.238 kb on + strand, within MMP_RS00845 at 159.669 kb on - strand, within MMP_RS00845 at 159.929 kb on - strand at 159.988 kb on - strand at 159.988 kb on - strand at 159.988 kb on - strand at 159.989 kb on + strand at 159.998 kb on + strand at 160.077 kb on + strand, within MMP_RS00850 at 160.119 kb on + strand, within MMP_RS00850 at 160.185 kb on + strand, within MMP_RS00850 at 160.344 kb on + strand, within MMP_RS00850 at 160.481 kb on - strand, within MMP_RS00850 at 160.542 kb on - strand, within MMP_RS00850 at 160.787 kb on - strand, within MMP_RS00850 at 161.052 kb on - strand at 161.052 kb on - strand at 161.052 kb on - strand at 161.059 kb on - strand at 161.060 kb on + strand at 161.067 kb on - strand at 161.067 kb on - strand at 161.067 kb on - strand at 161.068 kb on + strand at 161.068 kb on + strand at 161.069 kb on - strand at 161.069 kb on - strand at 161.069 kb on - strand at 161.070 kb on + strand at 161.070 kb on + strand at 161.071 kb on - strand at 161.071 kb on - strand at 161.071 kb on - strand at 161.072 kb on + strand at 161.072 kb on + strand at 161.100 kb on - strand at 161.101 kb on + strand at 161.102 kb on - strand at 161.102 kb on - strand at 161.134 kb on - strand at 161.134 kb on - strand at 161.176 kb on - strand, within MMP_RS00855 at 161.214 kb on - strand, within MMP_RS00855 at 161.260 kb on - strand, within MMP_RS00855 at 161.262 kb on - strand, within MMP_RS00855 at 161.408 kb on + strand, within MMP_RS00855 at 161.436 kb on + strand, within MMP_RS00855 at 161.436 kb on + strand, within MMP_RS00855 at 161.749 kb on + strand at 161.867 kb on + strand at 161.885 kb on + strand at 161.916 kb on - strand at 161.981 kb on + strand at 162.004 kb on + strand at 162.007 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM remove 159,061 - MMP_RS00845 0.54 +3.1 159,099 - MMP_RS00845 0.56 +1.2 159,165 + MMP_RS00845 0.60 +2.0 159,172 + MMP_RS00845 0.60 +0.1 159,232 - MMP_RS00845 0.63 +0.4 159,233 + MMP_RS00845 0.63 +0.4 159,235 - MMP_RS00845 0.64 -0.3 159,235 - MMP_RS00845 0.64 -0.2 159,237 - MMP_RS00845 0.64 -2.0 159,237 - MMP_RS00845 0.64 -0.1 159,237 - MMP_RS00845 0.64 -0.0 159,237 - MMP_RS00845 0.64 +0.4 159,238 + MMP_RS00845 0.64 -1.9 159,238 + MMP_RS00845 0.64 -0.3 159,669 - MMP_RS00845 0.86 +1.0 159,929 - +1.0 159,988 - -0.3 159,988 - -1.7 159,988 - -1.8 159,989 + +0.4 159,998 + -1.6 160,077 + MMP_RS00850 0.11 +0.5 160,119 + MMP_RS00850 0.15 -1.0 160,185 + MMP_RS00850 0.21 +0.4 160,344 + MMP_RS00850 0.36 -0.9 160,481 - MMP_RS00850 0.49 -2.6 160,542 - MMP_RS00850 0.55 -1.9 160,787 - MMP_RS00850 0.78 -3.9 161,052 - +1.0 161,052 - +0.2 161,052 - +0.9 161,059 - +1.2 161,060 + +0.7 161,067 - +0.6 161,067 - +1.6 161,067 - -1.9 161,068 + +1.1 161,068 + -1.8 161,069 - -0.8 161,069 - +0.7 161,069 - +1.8 161,070 + -0.3 161,070 + -1.2 161,071 - +0.7 161,071 - +0.9 161,071 - +0.4 161,072 + +0.5 161,072 + +0.8 161,100 - +2.1 161,101 + +1.4 161,102 - +0.6 161,102 - +0.5 161,134 - +0.1 161,134 - +0.4 161,176 - MMP_RS00855 0.17 +2.5 161,214 - MMP_RS00855 0.25 +1.0 161,260 - MMP_RS00855 0.34 +0.0 161,262 - MMP_RS00855 0.34 -0.9 161,408 + MMP_RS00855 0.62 -0.4 161,436 + MMP_RS00855 0.68 +0.0 161,436 + MMP_RS00855 0.68 +0.5 161,749 + -0.7 161,867 + -0.9 161,885 + +1.9 161,916 - -1.0 161,981 + +0.8 162,004 + -1.3 162,007 + -0.7
Or see this region's nucleotide sequence