Experiment: R2A_PIPES with Cobalt chloride 256 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS02515 and LRK54_RS02520 are separated by 96 nucleotides LRK54_RS02520 and LRK54_RS02525 are separated by 9 nucleotides LRK54_RS02525 and LRK54_RS02530 are separated by 113 nucleotides
LRK54_RS02515: LRK54_RS02515 - signal recognition particle protein, at 543,754 to 545,133
_RS02515
LRK54_RS02520: LRK54_RS02520 - cytochrome c biogenesis protein CcsA, at 545,230 to 546,027
_RS02520
LRK54_RS02525: LRK54_RS02525 - DNA repair protein RadA, at 546,037 to 547,422
_RS02525
LRK54_RS02530: LRK54_RS02530 - replicative DNA helicase, at 547,536 to 548,930
_RS02530
Position (kb) 546
547
548 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 545.166 kb on - strand at 545.167 kb on + strand at 545.167 kb on + strand at 545.167 kb on + strand at 545.167 kb on + strand at 545.168 kb on - strand at 545.168 kb on - strand at 545.168 kb on - strand at 545.168 kb on - strand at 545.269 kb on + strand at 545.269 kb on + strand at 545.269 kb on + strand at 545.269 kb on + strand at 545.269 kb on + strand at 545.269 kb on + strand at 545.269 kb on + strand at 545.270 kb on - strand at 545.270 kb on - strand at 545.270 kb on - strand at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.784 kb on + strand, within LRK54_RS02520 at 545.785 kb on - strand, within LRK54_RS02520 at 545.785 kb on - strand, within LRK54_RS02520 at 545.785 kb on - strand, within LRK54_RS02520 at 545.785 kb on - strand, within LRK54_RS02520 at 545.785 kb on - strand, within LRK54_RS02520 at 545.785 kb on - strand, within LRK54_RS02520 at 545.785 kb on - strand, within LRK54_RS02520 at 545.785 kb on - strand, within LRK54_RS02520 at 545.896 kb on + strand, within LRK54_RS02520 at 545.896 kb on + strand, within LRK54_RS02520 at 545.896 kb on + strand, within LRK54_RS02520 at 545.896 kb on + strand, within LRK54_RS02520 at 545.896 kb on + strand, within LRK54_RS02520 at 545.897 kb on - strand, within LRK54_RS02520 at 545.897 kb on - strand, within LRK54_RS02520 at 545.897 kb on - strand, within LRK54_RS02520 at 546.025 kb on + strand at 546.025 kb on + strand at 546.025 kb on + strand at 546.026 kb on - strand at 546.026 kb on - strand at 546.026 kb on - strand at 546.026 kb on - strand at 546.026 kb on - strand at 546.026 kb on - strand at 546.026 kb on - strand at 546.026 kb on - strand at 546.026 kb on - strand at 546.274 kb on + strand, within LRK54_RS02525 at 546.274 kb on + strand, within LRK54_RS02525 at 546.356 kb on + strand, within LRK54_RS02525 at 546.357 kb on - strand, within LRK54_RS02525 at 546.357 kb on - strand, within LRK54_RS02525 at 546.461 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.475 kb on + strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.476 kb on - strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.798 kb on - strand, within LRK54_RS02525 at 546.798 kb on - strand, within LRK54_RS02525 at 546.863 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 256 uM remove 545,166 - -1.4 545,167 + +0.2 545,167 + -1.3 545,167 + -1.1 545,167 + -0.8 545,168 - -1.5 545,168 - -0.2 545,168 - -1.2 545,168 - +0.4 545,269 + -1.9 545,269 + +1.4 545,269 + -1.0 545,269 + -0.6 545,269 + -2.2 545,269 + +1.8 545,269 + -1.5 545,270 - -4.2 545,270 - -0.2 545,270 - -2.4 545,784 + LRK54_RS02520 0.69 -0.5 545,784 + LRK54_RS02520 0.69 -3.6 545,784 + LRK54_RS02520 0.69 -0.6 545,784 + LRK54_RS02520 0.69 -2.5 545,784 + LRK54_RS02520 0.69 -0.4 545,784 + LRK54_RS02520 0.69 -0.9 545,784 + LRK54_RS02520 0.69 -1.5 545,784 + LRK54_RS02520 0.69 -2.4 545,784 + LRK54_RS02520 0.69 -1.7 545,784 + LRK54_RS02520 0.69 +0.8 545,784 + LRK54_RS02520 0.69 -1.3 545,784 + LRK54_RS02520 0.69 +0.2 545,784 + LRK54_RS02520 0.69 -0.1 545,785 - LRK54_RS02520 0.70 -2.6 545,785 - LRK54_RS02520 0.70 -0.9 545,785 - LRK54_RS02520 0.70 +0.8 545,785 - LRK54_RS02520 0.70 -1.7 545,785 - LRK54_RS02520 0.70 -1.6 545,785 - LRK54_RS02520 0.70 -1.4 545,785 - LRK54_RS02520 0.70 -2.2 545,785 - LRK54_RS02520 0.70 -2.4 545,896 + LRK54_RS02520 0.83 -2.0 545,896 + LRK54_RS02520 0.83 -2.3 545,896 + LRK54_RS02520 0.83 -3.9 545,896 + LRK54_RS02520 0.83 -0.2 545,896 + LRK54_RS02520 0.83 +0.1 545,897 - LRK54_RS02520 0.84 -2.5 545,897 - LRK54_RS02520 0.84 -1.4 545,897 - LRK54_RS02520 0.84 -3.5 546,025 + -0.4 546,025 + -0.5 546,025 + -0.3 546,026 - -0.5 546,026 - -0.2 546,026 - -0.4 546,026 - -1.8 546,026 - +0.3 546,026 - +1.8 546,026 - +0.1 546,026 - +0.3 546,026 - -0.9 546,274 + LRK54_RS02525 0.17 -1.1 546,274 + LRK54_RS02525 0.17 -0.0 546,356 + LRK54_RS02525 0.23 -0.7 546,357 - LRK54_RS02525 0.23 -0.2 546,357 - LRK54_RS02525 0.23 -2.5 546,461 + LRK54_RS02525 0.31 -2.3 546,475 + LRK54_RS02525 0.32 -0.8 546,475 + LRK54_RS02525 0.32 +0.1 546,475 + LRK54_RS02525 0.32 -0.7 546,475 + LRK54_RS02525 0.32 -0.8 546,475 + LRK54_RS02525 0.32 -1.2 546,475 + LRK54_RS02525 0.32 -0.1 546,475 + LRK54_RS02525 0.32 +0.2 546,475 + LRK54_RS02525 0.32 +0.1 546,475 + LRK54_RS02525 0.32 -1.0 546,475 + LRK54_RS02525 0.32 +0.0 546,475 + LRK54_RS02525 0.32 -1.9 546,475 + LRK54_RS02525 0.32 -3.1 546,475 + LRK54_RS02525 0.32 -0.2 546,475 + LRK54_RS02525 0.32 -0.8 546,475 + LRK54_RS02525 0.32 -0.2 546,476 - LRK54_RS02525 0.32 -1.2 546,476 - LRK54_RS02525 0.32 +0.4 546,476 - LRK54_RS02525 0.32 -1.4 546,476 - LRK54_RS02525 0.32 +0.3 546,476 - LRK54_RS02525 0.32 -0.7 546,476 - LRK54_RS02525 0.32 +0.9 546,476 - LRK54_RS02525 0.32 -0.1 546,476 - LRK54_RS02525 0.32 -0.7 546,476 - LRK54_RS02525 0.32 +0.8 546,476 - LRK54_RS02525 0.32 +1.7 546,476 - LRK54_RS02525 0.32 -0.3 546,476 - LRK54_RS02525 0.32 -1.1 546,476 - LRK54_RS02525 0.32 +0.6 546,476 - LRK54_RS02525 0.32 -0.5 546,692 + LRK54_RS02525 0.47 -2.9 546,692 + LRK54_RS02525 0.47 -0.2 546,692 + LRK54_RS02525 0.47 +1.0 546,692 + LRK54_RS02525 0.47 -1.2 546,692 + LRK54_RS02525 0.47 +1.1 546,692 + LRK54_RS02525 0.47 +0.1 546,692 + LRK54_RS02525 0.47 +0.2 546,693 - LRK54_RS02525 0.47 -0.2 546,693 - LRK54_RS02525 0.47 +0.0 546,693 - LRK54_RS02525 0.47 -1.0 546,693 - LRK54_RS02525 0.47 -0.3 546,693 - LRK54_RS02525 0.47 -0.1 546,693 - LRK54_RS02525 0.47 +0.5 546,693 - LRK54_RS02525 0.47 -2.1 546,693 - LRK54_RS02525 0.47 +3.1 546,693 - LRK54_RS02525 0.47 -0.1 546,797 + LRK54_RS02525 0.55 +0.5 546,797 + LRK54_RS02525 0.55 +2.4 546,797 + LRK54_RS02525 0.55 -0.5 546,797 + LRK54_RS02525 0.55 +0.3 546,797 + LRK54_RS02525 0.55 -0.5 546,798 - LRK54_RS02525 0.55 +0.5 546,798 - LRK54_RS02525 0.55 +0.6 546,863 + LRK54_RS02525 0.60 -1.8 547,037 + LRK54_RS02525 0.72 +0.5 547,037 + LRK54_RS02525 0.72 -0.3 547,037 + LRK54_RS02525 0.72 -0.1 547,037 + LRK54_RS02525 0.72 -0.2 547,037 + LRK54_RS02525 0.72 -0.9 547,037 + LRK54_RS02525 0.72 -0.6 547,037 + LRK54_RS02525 0.72 -0.7 547,037 + LRK54_RS02525 0.72 +0.7 547,037 + LRK54_RS02525 0.72 +0.6 547,037 + LRK54_RS02525 0.72 -2.0 547,037 + LRK54_RS02525 0.72 -0.4 547,037 + LRK54_RS02525 0.72 -0.9 547,037 + LRK54_RS02525 0.72 +0.3 547,037 + LRK54_RS02525 0.72 +1.6 547,037 + LRK54_RS02525 0.72 -0.8 547,037 + LRK54_RS02525 0.72 +0.7 547,037 + LRK54_RS02525 0.72 -1.1 547,037 + LRK54_RS02525 0.72 -0.5 547,037 + LRK54_RS02525 0.72 -1.3 547,037 + LRK54_RS02525 0.72 +0.8 547,037 + LRK54_RS02525 0.72 +1.0 547,037 + LRK54_RS02525 0.72 -0.5 547,038 - LRK54_RS02525 0.72 -0.2 547,038 - LRK54_RS02525 0.72 -1.5 547,038 - LRK54_RS02525 0.72 -0.0 547,038 - LRK54_RS02525 0.72 +2.4 547,038 - LRK54_RS02525 0.72 -0.8 547,038 - LRK54_RS02525 0.72 +0.7 547,038 - LRK54_RS02525 0.72 +1.5 547,038 - LRK54_RS02525 0.72 +0.1 547,038 - LRK54_RS02525 0.72 -0.2 547,038 - LRK54_RS02525 0.72 +0.2 547,038 - LRK54_RS02525 0.72 +0.1 547,038 - LRK54_RS02525 0.72 -0.2 547,038 - LRK54_RS02525 0.72 -0.9 547,038 - LRK54_RS02525 0.72 -1.3 547,038 - LRK54_RS02525 0.72 -1.1 547,038 - LRK54_RS02525 0.72 -0.0 547,038 - LRK54_RS02525 0.72 -2.1 547,038 - LRK54_RS02525 0.72 +0.8 547,038 - LRK54_RS02525 0.72 +0.8 547,038 - LRK54_RS02525 0.72 -0.9 547,259 + LRK54_RS02525 0.88 -1.0 547,259 + LRK54_RS02525 0.88 -0.8 547,259 + LRK54_RS02525 0.88 +1.8 547,259 + LRK54_RS02525 0.88 +1.8 547,259 + LRK54_RS02525 0.88 -0.3 547,260 - LRK54_RS02525 0.88 +0.9 547,260 - LRK54_RS02525 0.88 +0.4 547,260 - LRK54_RS02525 0.88 +0.1 547,260 - LRK54_RS02525 0.88 +0.9 547,260 - LRK54_RS02525 0.88 -0.4 547,260 - LRK54_RS02525 0.88 -0.1 547,260 - LRK54_RS02525 0.88 -1.2 547,400 + -0.5 547,400 + +1.0 547,400 + +0.4 547,400 + +0.9 547,400 + -0.6 547,400 + -0.2 547,400 + +0.4 547,400 + +0.2 547,400 + -0.3 547,400 + +0.3 547,400 + -0.5 547,400 + -1.0 547,400 + -1.0 547,400 + +0.1 547,401 - -0.8 547,401 - -1.0 547,401 - +0.2 547,401 - +0.4 547,401 - -0.4 547,401 - -2.0 547,401 - -1.3 547,401 - +1.4 547,401 - -1.5 547,489 + +1.2 547,489 + -1.3 547,489 + +0.3 547,489 + +0.3 547,489 + +1.1 547,489 + +0.0 547,489 + -0.9 547,489 + -1.1 547,489 + +0.5 547,490 - +0.6 547,490 - -1.0 547,490 - +0.0 547,490 - +0.9 547,490 - +0.8 547,490 - +2.5 547,490 - +0.8 547,490 - -0.5 547,490 - +0.6 547,490 - -1.2 547,490 - -1.3 547,490 - -0.2 547,490 - -0.6 547,490 - +0.4 547,490 - +0.3 547,490 - +0.1 547,570 + -0.4 547,570 + +0.8 547,570 + +0.4 547,570 + -0.3 547,570 + -1.3 547,570 + -1.4 547,570 + -1.3 547,570 + -0.4 547,571 - +0.3 547,571 - +0.1 547,571 - +0.8 547,571 - +0.9 547,571 - -2.6 547,571 - -0.2 547,571 - +0.2 547,571 - -1.0
Or see this region's nucleotide sequence