Experiment: Columbia 1x (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06050 and HMPREF1058_RS06045 are separated by 151 nucleotides HMPREF1058_RS06045 and HMPREF1058_RS06040 are separated by 237 nucleotides HMPREF1058_RS06040 and HMPREF1058_RS06035 are separated by 38 nucleotides
HMPREF1058_RS06050: HMPREF1058_RS06050 - type I glyceraldehyde-3-phosphate dehydrogenase, at 15,811 to 16,821
_RS06050
HMPREF1058_RS06045: HMPREF1058_RS06045 - large-conductance mechanosensitive channel protein MscL, at 16,973 to 17,413
_RS06045
HMPREF1058_RS06040: HMPREF1058_RS06040 - glutamine-hydrolyzing GMP synthase, at 17,651 to 19,204
_RS06040
HMPREF1058_RS06035: HMPREF1058_RS06035 - glutamine-hydrolyzing GMP synthase, at 19,243 to 20,550
_RS06035
Position (kb) 17
18
19
20 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 16.852 kb on - strand at 16.899 kb on - strand at 16.899 kb on - strand at 17.128 kb on - strand, within HMPREF1058_RS06045 at 17.158 kb on - strand, within HMPREF1058_RS06045 at 17.158 kb on - strand, within HMPREF1058_RS06045 at 17.163 kb on + strand, within HMPREF1058_RS06045 at 17.164 kb on - strand, within HMPREF1058_RS06045 at 17.217 kb on - strand, within HMPREF1058_RS06045 at 17.268 kb on + strand, within HMPREF1058_RS06045 at 17.275 kb on - strand, within HMPREF1058_RS06045 at 17.364 kb on + strand, within HMPREF1058_RS06045 at 17.364 kb on + strand, within HMPREF1058_RS06045 at 17.365 kb on - strand, within HMPREF1058_RS06045 at 17.368 kb on + strand, within HMPREF1058_RS06045 at 17.472 kb on + strand at 17.473 kb on - strand at 17.473 kb on - strand at 17.474 kb on + strand at 17.475 kb on - strand at 17.567 kb on + strand at 17.568 kb on - strand at 17.609 kb on + strand at 17.636 kb on + strand at 17.636 kb on + strand at 17.819 kb on + strand, within HMPREF1058_RS06040 at 18.096 kb on + strand, within HMPREF1058_RS06040 at 18.132 kb on + strand, within HMPREF1058_RS06040 at 18.132 kb on + strand, within HMPREF1058_RS06040 at 18.141 kb on + strand, within HMPREF1058_RS06040 at 18.297 kb on + strand, within HMPREF1058_RS06040 at 18.426 kb on + strand, within HMPREF1058_RS06040 at 18.459 kb on + strand, within HMPREF1058_RS06040 at 18.639 kb on + strand, within HMPREF1058_RS06040 at 18.711 kb on + strand, within HMPREF1058_RS06040 at 18.756 kb on + strand, within HMPREF1058_RS06040 at 18.756 kb on + strand, within HMPREF1058_RS06040 at 18.772 kb on + strand, within HMPREF1058_RS06040 at 18.829 kb on + strand, within HMPREF1058_RS06040 at 18.887 kb on + strand, within HMPREF1058_RS06040 at 18.887 kb on + strand, within HMPREF1058_RS06040 at 18.887 kb on + strand, within HMPREF1058_RS06040 at 18.887 kb on + strand, within HMPREF1058_RS06040 at 18.887 kb on + strand, within HMPREF1058_RS06040 at 18.957 kb on + strand, within HMPREF1058_RS06040 at 18.957 kb on + strand, within HMPREF1058_RS06040 at 18.957 kb on + strand, within HMPREF1058_RS06040 at 19.002 kb on + strand, within HMPREF1058_RS06040 at 19.002 kb on + strand at 19.018 kb on + strand, within HMPREF1058_RS06040 at 19.137 kb on + strand at 19.137 kb on + strand at 19.155 kb on + strand at 19.217 kb on + strand at 19.225 kb on + strand at 19.225 kb on + strand at 19.254 kb on + strand at 19.263 kb on + strand at 19.325 kb on + strand at 19.326 kb on - strand at 19.460 kb on - strand, within HMPREF1058_RS06035 at 19.670 kb on + strand, within HMPREF1058_RS06035 at 19.694 kb on + strand, within HMPREF1058_RS06035 at 19.754 kb on + strand, within HMPREF1058_RS06035 at 19.755 kb on - strand, within HMPREF1058_RS06035 at 19.755 kb on - strand, within HMPREF1058_RS06035 at 19.986 kb on + strand, within HMPREF1058_RS06035 at 20.020 kb on - strand, within HMPREF1058_RS06035 at 20.054 kb on + strand, within HMPREF1058_RS06035 at 20.055 kb on - strand, within HMPREF1058_RS06035 at 20.055 kb on - strand, within HMPREF1058_RS06035 at 20.150 kb on + strand, within HMPREF1058_RS06035
Per-strain Table
Position Strand Gene LocusTag Fraction Columbia 1x (C) complex media remove 16,852 - -1.7 16,899 - +2.1 16,899 - +1.5 17,128 - HMPREF1058_RS06045 0.35 -2.3 17,158 - HMPREF1058_RS06045 0.42 -0.2 17,158 - HMPREF1058_RS06045 0.42 +0.1 17,163 + HMPREF1058_RS06045 0.43 -0.1 17,164 - HMPREF1058_RS06045 0.43 +1.1 17,217 - HMPREF1058_RS06045 0.55 +0.2 17,268 + HMPREF1058_RS06045 0.67 -0.0 17,275 - HMPREF1058_RS06045 0.68 -0.2 17,364 + HMPREF1058_RS06045 0.89 -1.1 17,364 + HMPREF1058_RS06045 0.89 -0.3 17,365 - HMPREF1058_RS06045 0.89 -0.1 17,368 + HMPREF1058_RS06045 0.90 -0.2 17,472 + -1.4 17,473 - +0.7 17,473 - -0.3 17,474 + +0.3 17,475 - +0.8 17,567 + -0.2 17,568 - -0.0 17,609 + -0.4 17,636 + +1.1 17,636 + -0.9 17,819 + HMPREF1058_RS06040 0.11 -0.9 18,096 + HMPREF1058_RS06040 0.29 -0.3 18,132 + HMPREF1058_RS06040 0.31 +0.7 18,132 + HMPREF1058_RS06040 0.31 -1.3 18,141 + HMPREF1058_RS06040 0.32 -2.7 18,297 + HMPREF1058_RS06040 0.42 -0.5 18,426 + HMPREF1058_RS06040 0.50 +1.8 18,459 + HMPREF1058_RS06040 0.52 +0.5 18,639 + HMPREF1058_RS06040 0.64 -1.3 18,711 + HMPREF1058_RS06040 0.68 -0.5 18,756 + HMPREF1058_RS06040 0.71 -0.2 18,756 + HMPREF1058_RS06040 0.71 -1.7 18,772 + HMPREF1058_RS06040 0.72 +0.7 18,829 + HMPREF1058_RS06040 0.76 -0.7 18,887 + HMPREF1058_RS06040 0.80 -1.0 18,887 + HMPREF1058_RS06040 0.80 -1.2 18,887 + HMPREF1058_RS06040 0.80 -3.2 18,887 + HMPREF1058_RS06040 0.80 +0.1 18,887 + HMPREF1058_RS06040 0.80 -0.7 18,957 + HMPREF1058_RS06040 0.84 -0.0 18,957 + HMPREF1058_RS06040 0.84 -0.3 18,957 + HMPREF1058_RS06040 0.84 -0.5 19,002 + HMPREF1058_RS06040 0.87 +2.2 19,002 + -0.1 19,018 + HMPREF1058_RS06040 0.88 -0.1 19,137 + -0.9 19,137 + -0.7 19,155 + -1.0 19,217 + -1.3 19,225 + +0.7 19,225 + -1.3 19,254 + -0.5 19,263 + -1.6 19,325 + -1.7 19,326 - -1.4 19,460 - HMPREF1058_RS06035 0.17 +0.5 19,670 + HMPREF1058_RS06035 0.33 -0.7 19,694 + HMPREF1058_RS06035 0.34 -2.2 19,754 + HMPREF1058_RS06035 0.39 +0.1 19,755 - HMPREF1058_RS06035 0.39 -0.3 19,755 - HMPREF1058_RS06035 0.39 -2.1 19,986 + HMPREF1058_RS06035 0.57 -0.4 20,020 - HMPREF1058_RS06035 0.59 -0.8 20,054 + HMPREF1058_RS06035 0.62 -1.4 20,055 - HMPREF1058_RS06035 0.62 -1.7 20,055 - HMPREF1058_RS06035 0.62 +0.2 20,150 + HMPREF1058_RS06035 0.69 -0.8
Or see this region's nucleotide sequence