Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS20205 and HMPREF1058_RS18585 are separated by 401 nucleotides HMPREF1058_RS18585 and HMPREF1058_RS18580 are separated by 30 nucleotides HMPREF1058_RS18580 and HMPREF1058_RS18575 are separated by 230 nucleotides HMPREF1058_RS18575 and HMPREF1058_RS18570 overlap by 8 nucleotides HMPREF1058_RS18570 and HMPREF1058_RS18565 overlap by 4 nucleotides
HMPREF1058_RS20205: HMPREF1058_RS20205 - energy transducer TonB, at 122,816 to 124,270
_RS20205
HMPREF1058_RS18585: HMPREF1058_RS18585 - tRNA-Asp, at 124,672 to 124,745
_RS18585
HMPREF1058_RS18580: HMPREF1058_RS18580 - tRNA-Asp, at 124,776 to 124,849
_RS18580
HMPREF1058_RS18575: HMPREF1058_RS18575 - YqgE/AlgH family protein, at 125,080 to 125,670
_RS18575
HMPREF1058_RS18570: HMPREF1058_RS18570 - GNAT family N-acetyltransferase, at 125,663 to 126,199
_RS18570
HMPREF1058_RS18565: HMPREF1058_RS18565 - hypothetical protein, at 126,196 to 126,666
_RS18565
Position (kb) 125
126 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 124.102 kb on + strand, within HMPREF1058_RS20205 at 124.102 kb on + strand, within HMPREF1058_RS20205 at 124.103 kb on - strand, within HMPREF1058_RS20205 at 124.105 kb on + strand, within HMPREF1058_RS20205 at 124.106 kb on - strand, within HMPREF1058_RS20205 at 124.108 kb on - strand, within HMPREF1058_RS20205 at 124.119 kb on + strand, within HMPREF1058_RS20205 at 124.210 kb on - strand at 124.215 kb on - strand at 124.216 kb on + strand at 124.217 kb on - strand at 124.222 kb on - strand at 124.226 kb on - strand at 124.226 kb on - strand at 124.226 kb on - strand at 124.244 kb on - strand at 124.336 kb on + strand at 124.353 kb on - strand at 124.360 kb on + strand at 124.439 kb on + strand at 124.440 kb on - strand at 124.446 kb on + strand at 124.509 kb on + strand at 124.510 kb on - strand at 124.572 kb on + strand at 124.979 kb on + strand at 124.984 kb on + strand at 125.037 kb on + strand at 125.038 kb on - strand at 125.043 kb on - strand at 125.046 kb on + strand at 125.046 kb on + strand at 125.047 kb on - strand at 125.047 kb on - strand at 125.047 kb on - strand at 125.047 kb on - strand at 125.048 kb on + strand at 125.176 kb on - strand, within HMPREF1058_RS18575 at 125.220 kb on + strand, within HMPREF1058_RS18575 at 125.226 kb on + strand, within HMPREF1058_RS18575 at 125.312 kb on - strand, within HMPREF1058_RS18575 at 125.434 kb on + strand, within HMPREF1058_RS18575 at 125.434 kb on + strand, within HMPREF1058_RS18575 at 125.435 kb on - strand, within HMPREF1058_RS18575 at 125.435 kb on - strand, within HMPREF1058_RS18575 at 125.436 kb on + strand, within HMPREF1058_RS18575 at 125.437 kb on - strand, within HMPREF1058_RS18575 at 125.437 kb on - strand, within HMPREF1058_RS18575 at 125.523 kb on - strand, within HMPREF1058_RS18575 at 125.591 kb on + strand, within HMPREF1058_RS18575 at 125.720 kb on - strand, within HMPREF1058_RS18570 at 125.806 kb on - strand, within HMPREF1058_RS18570 at 125.806 kb on - strand, within HMPREF1058_RS18570 at 125.952 kb on - strand, within HMPREF1058_RS18570 at 126.041 kb on + strand at 126.042 kb on - strand, within HMPREF1058_RS18570 at 126.045 kb on + strand, within HMPREF1058_RS18570 at 126.046 kb on - strand, within HMPREF1058_RS18570 at 126.382 kb on + strand, within HMPREF1058_RS18565 at 126.525 kb on - strand, within HMPREF1058_RS18565 at 126.525 kb on - strand, within HMPREF1058_RS18565 at 126.620 kb on + strand at 126.620 kb on + strand at 126.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 124,102 + HMPREF1058_RS20205 0.88 -0.2 124,102 + HMPREF1058_RS20205 0.88 +2.0 124,103 - HMPREF1058_RS20205 0.88 -1.4 124,105 + HMPREF1058_RS20205 0.89 -0.1 124,106 - HMPREF1058_RS20205 0.89 -0.6 124,108 - HMPREF1058_RS20205 0.89 +0.1 124,119 + HMPREF1058_RS20205 0.90 -1.0 124,210 - -2.4 124,215 - +1.1 124,216 + -0.0 124,217 - +0.5 124,222 - +0.4 124,226 - +0.1 124,226 - -0.4 124,226 - -0.3 124,244 - +0.6 124,336 + -0.2 124,353 - +1.0 124,360 + +0.1 124,439 + -1.9 124,440 - -1.2 124,446 + -1.5 124,509 + -1.8 124,510 - +0.3 124,572 + -1.2 124,979 + +1.2 124,984 + +0.9 125,037 + -1.2 125,038 - -0.9 125,043 - +0.7 125,046 + -1.4 125,046 + -0.4 125,047 - -0.4 125,047 - +0.3 125,047 - +0.5 125,047 - +0.2 125,048 + -1.8 125,176 - HMPREF1058_RS18575 0.16 +1.7 125,220 + HMPREF1058_RS18575 0.24 -1.6 125,226 + HMPREF1058_RS18575 0.25 +0.6 125,312 - HMPREF1058_RS18575 0.39 -0.3 125,434 + HMPREF1058_RS18575 0.60 +0.9 125,434 + HMPREF1058_RS18575 0.60 +1.1 125,435 - HMPREF1058_RS18575 0.60 -0.5 125,435 - HMPREF1058_RS18575 0.60 +1.2 125,436 + HMPREF1058_RS18575 0.60 +1.0 125,437 - HMPREF1058_RS18575 0.60 +0.3 125,437 - HMPREF1058_RS18575 0.60 +0.5 125,523 - HMPREF1058_RS18575 0.75 +0.9 125,591 + HMPREF1058_RS18575 0.86 +1.2 125,720 - HMPREF1058_RS18570 0.11 +1.2 125,806 - HMPREF1058_RS18570 0.27 -1.2 125,806 - HMPREF1058_RS18570 0.27 -4.3 125,952 - HMPREF1058_RS18570 0.54 -0.2 126,041 + -0.6 126,042 - HMPREF1058_RS18570 0.71 -1.3 126,045 + HMPREF1058_RS18570 0.71 +0.2 126,046 - HMPREF1058_RS18570 0.71 -1.8 126,382 + HMPREF1058_RS18565 0.39 -2.5 126,525 - HMPREF1058_RS18565 0.70 -0.4 126,525 - HMPREF1058_RS18565 0.70 +0.5 126,620 + -2.2 126,620 + -0.9 126,621 - -0.7
Or see this region's nucleotide sequence