Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS17985 and HMPREF1058_RS17980 are separated by 724 nucleotides HMPREF1058_RS17980 and HMPREF1058_RS20410 are separated by 62 nucleotides HMPREF1058_RS20410 and HMPREF1058_RS17975 are separated by 148 nucleotides
HMPREF1058_RS17985: HMPREF1058_RS17985 - ATP-binding cassette domain-containing protein, at 285,482 to 286,930
_RS17985
HMPREF1058_RS17980: HMPREF1058_RS17980 - hypothetical protein, at 287,655 to 288,380
_RS17980
HMPREF1058_RS20410: HMPREF1058_RS20410 - hypothetical protein, at 288,443 to 288,675
_RS20410
HMPREF1058_RS17975: HMPREF1058_RS17975 - ATP-dependent dethiobiotin synthetase BioD, at 288,824 to 289,468
_RS17975
Position (kb)
287
288
289 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 286.695 kb on + strand, within HMPREF1058_RS17985 at 286.702 kb on + strand, within HMPREF1058_RS17985 at 286.791 kb on - strand at 286.832 kb on + strand at 286.832 kb on + strand at 286.834 kb on + strand at 286.835 kb on - strand at 286.835 kb on - strand at 286.859 kb on - strand at 286.994 kb on - strand at 287.027 kb on + strand at 287.028 kb on - strand at 287.032 kb on - strand at 287.050 kb on - strand at 287.076 kb on + strand at 287.088 kb on - strand at 287.243 kb on + strand at 287.243 kb on + strand at 287.244 kb on - strand at 287.247 kb on + strand at 287.249 kb on + strand at 287.249 kb on + strand at 287.249 kb on - strand at 287.272 kb on + strand at 287.272 kb on + strand at 287.315 kb on + strand at 287.315 kb on + strand at 287.450 kb on - strand at 287.473 kb on - strand at 287.475 kb on - strand at 287.475 kb on - strand at 287.561 kb on + strand at 287.571 kb on + strand at 287.572 kb on - strand at 287.588 kb on + strand at 287.603 kb on + strand at 287.607 kb on + strand at 287.608 kb on - strand at 287.616 kb on + strand at 287.616 kb on + strand at 287.617 kb on - strand at 287.628 kb on + strand at 287.628 kb on + strand at 287.629 kb on - strand at 287.764 kb on + strand, within HMPREF1058_RS17980 at 287.772 kb on + strand, within HMPREF1058_RS17980 at 287.886 kb on - strand, within HMPREF1058_RS17980 at 287.908 kb on + strand, within HMPREF1058_RS17980 at 287.909 kb on - strand, within HMPREF1058_RS17980 at 287.997 kb on - strand, within HMPREF1058_RS17980 at 288.022 kb on + strand, within HMPREF1058_RS17980 at 288.023 kb on - strand, within HMPREF1058_RS17980 at 288.023 kb on - strand, within HMPREF1058_RS17980 at 288.133 kb on + strand, within HMPREF1058_RS17980 at 288.133 kb on + strand, within HMPREF1058_RS17980 at 288.202 kb on - strand, within HMPREF1058_RS17980 at 288.224 kb on - strand, within HMPREF1058_RS17980 at 288.354 kb on + strand at 288.384 kb on + strand at 288.451 kb on - strand at 288.455 kb on - strand at 288.467 kb on + strand, within HMPREF1058_RS20410 at 288.490 kb on + strand, within HMPREF1058_RS20410 at 288.490 kb on + strand, within HMPREF1058_RS20410 at 288.491 kb on - strand, within HMPREF1058_RS20410 at 288.493 kb on + strand, within HMPREF1058_RS20410 at 288.520 kb on - strand, within HMPREF1058_RS20410 at 288.624 kb on + strand, within HMPREF1058_RS20410 at 288.625 kb on - strand, within HMPREF1058_RS20410 at 288.652 kb on + strand at 288.652 kb on + strand at 288.652 kb on + strand at 288.673 kb on + strand at 288.719 kb on + strand at 288.719 kb on + strand at 288.721 kb on + strand at 288.722 kb on - strand at 288.726 kb on + strand at 288.727 kb on - strand at 288.744 kb on + strand at 288.745 kb on - strand at 288.773 kb on + strand at 288.836 kb on + strand at 288.875 kb on - strand at 288.887 kb on - strand at 288.899 kb on - strand, within HMPREF1058_RS17975 at 288.899 kb on - strand, within HMPREF1058_RS17975 at 288.942 kb on + strand, within HMPREF1058_RS17975 at 288.942 kb on + strand, within HMPREF1058_RS17975 at 288.951 kb on + strand, within HMPREF1058_RS17975 at 288.952 kb on + strand, within HMPREF1058_RS17975 at 288.977 kb on + strand, within HMPREF1058_RS17975 at 288.978 kb on - strand, within HMPREF1058_RS17975 at 289.000 kb on + strand, within HMPREF1058_RS17975 at 289.009 kb on + strand, within HMPREF1058_RS17975 at 289.009 kb on + strand, within HMPREF1058_RS17975 at 289.065 kb on + strand, within HMPREF1058_RS17975 at 289.070 kb on + strand, within HMPREF1058_RS17975 at 289.071 kb on - strand, within HMPREF1058_RS17975 at 289.081 kb on + strand, within HMPREF1058_RS17975 at 289.122 kb on - strand, within HMPREF1058_RS17975 at 289.138 kb on - strand, within HMPREF1058_RS17975 at 289.278 kb on + strand, within HMPREF1058_RS17975 at 289.279 kb on - strand, within HMPREF1058_RS17975 at 289.363 kb on + strand, within HMPREF1058_RS17975 at 289.367 kb on - strand, within HMPREF1058_RS17975
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 286,695 + HMPREF1058_RS17985 0.84 +1.1 286,702 + HMPREF1058_RS17985 0.84 +0.2 286,791 - +0.1 286,832 + +0.9 286,832 + -0.2 286,834 + +0.6 286,835 - +0.0 286,835 - +1.6 286,859 - -0.1 286,994 - -1.6 287,027 + +0.7 287,028 - +0.6 287,032 - +3.0 287,050 - -0.2 287,076 + -1.9 287,088 - +0.1 287,243 + -0.0 287,243 + +0.5 287,244 - +0.7 287,247 + -0.6 287,249 + -1.5 287,249 + +1.7 287,249 - +1.0 287,272 + +0.8 287,272 + +0.7 287,315 + +0.7 287,315 + +0.8 287,450 - +1.7 287,473 - +0.1 287,475 - +0.5 287,475 - -0.1 287,561 + +0.9 287,571 + -0.5 287,572 - +1.5 287,588 + +2.1 287,603 + -0.2 287,607 + -1.1 287,608 - -0.9 287,616 + +0.3 287,616 + +0.6 287,617 - +1.2 287,628 + +1.7 287,628 + -2.4 287,629 - -0.7 287,764 + HMPREF1058_RS17980 0.15 +0.1 287,772 + HMPREF1058_RS17980 0.16 +0.6 287,886 - HMPREF1058_RS17980 0.32 +1.3 287,908 + HMPREF1058_RS17980 0.35 +1.0 287,909 - HMPREF1058_RS17980 0.35 -0.1 287,997 - HMPREF1058_RS17980 0.47 -0.0 288,022 + HMPREF1058_RS17980 0.51 -0.3 288,023 - HMPREF1058_RS17980 0.51 +0.4 288,023 - HMPREF1058_RS17980 0.51 +1.5 288,133 + HMPREF1058_RS17980 0.66 +1.4 288,133 + HMPREF1058_RS17980 0.66 +0.2 288,202 - HMPREF1058_RS17980 0.75 -1.4 288,224 - HMPREF1058_RS17980 0.78 +0.8 288,354 + -0.4 288,384 + +0.1 288,451 - +1.0 288,455 - +0.6 288,467 + HMPREF1058_RS20410 0.10 -0.4 288,490 + HMPREF1058_RS20410 0.20 +0.5 288,490 + HMPREF1058_RS20410 0.20 -0.4 288,491 - HMPREF1058_RS20410 0.21 +1.2 288,493 + HMPREF1058_RS20410 0.21 +2.8 288,520 - HMPREF1058_RS20410 0.33 -1.2 288,624 + HMPREF1058_RS20410 0.78 +1.3 288,625 - HMPREF1058_RS20410 0.78 +0.5 288,652 + +1.2 288,652 + +1.0 288,652 + +0.2 288,673 + -1.4 288,719 + -0.6 288,719 + +0.5 288,721 + -1.4 288,722 - -1.2 288,726 + +0.0 288,727 - -1.7 288,744 + -0.6 288,745 - +0.4 288,773 + -1.4 288,836 + -1.5 288,875 - -1.3 288,887 - -1.4 288,899 - HMPREF1058_RS17975 0.12 -0.5 288,899 - HMPREF1058_RS17975 0.12 -1.3 288,942 + HMPREF1058_RS17975 0.18 -1.3 288,942 + HMPREF1058_RS17975 0.18 -1.9 288,951 + HMPREF1058_RS17975 0.20 -1.3 288,952 + HMPREF1058_RS17975 0.20 -1.6 288,977 + HMPREF1058_RS17975 0.24 -3.7 288,978 - HMPREF1058_RS17975 0.24 -1.0 289,000 + HMPREF1058_RS17975 0.27 -1.4 289,009 + HMPREF1058_RS17975 0.29 -1.5 289,009 + HMPREF1058_RS17975 0.29 -0.4 289,065 + HMPREF1058_RS17975 0.37 -1.9 289,070 + HMPREF1058_RS17975 0.38 -2.8 289,071 - HMPREF1058_RS17975 0.38 -0.8 289,081 + HMPREF1058_RS17975 0.40 -2.1 289,122 - HMPREF1058_RS17975 0.46 -1.0 289,138 - HMPREF1058_RS17975 0.49 +1.1 289,278 + HMPREF1058_RS17975 0.70 -1.5 289,279 - HMPREF1058_RS17975 0.71 -1.5 289,363 + HMPREF1058_RS17975 0.84 -0.9 289,367 - HMPREF1058_RS17975 0.84 -0.9
Or see this region's nucleotide sequence