Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS15215 and HMPREF1058_RS15210 are separated by 89 nucleotides HMPREF1058_RS15210 and HMPREF1058_RS15205 overlap by 17 nucleotides HMPREF1058_RS15205 and HMPREF1058_RS15200 are separated by 341 nucleotides
HMPREF1058_RS15215: HMPREF1058_RS15215 - hypothetical protein, at 972,784 to 973,926
_RS15215
HMPREF1058_RS15210: HMPREF1058_RS15210 - aminotransferase class IV, at 974,016 to 974,609
_RS15210
HMPREF1058_RS15205: HMPREF1058_RS15205 - aminodeoxychorismate synthase component I, at 974,593 to 975,576
_RS15205
HMPREF1058_RS15200: HMPREF1058_RS15200 - hypothetical protein, at 975,918 to 977,951
_RS15200
Position (kb)
974
975
976 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 973.618 kb on - strand, within HMPREF1058_RS15215 at 973.647 kb on + strand, within HMPREF1058_RS15215 at 973.678 kb on - strand, within HMPREF1058_RS15215 at 973.718 kb on + strand, within HMPREF1058_RS15215 at 973.752 kb on + strand, within HMPREF1058_RS15215 at 973.752 kb on + strand, within HMPREF1058_RS15215 at 973.753 kb on - strand, within HMPREF1058_RS15215 at 973.755 kb on + strand, within HMPREF1058_RS15215 at 973.843 kb on - strand at 973.843 kb on - strand at 973.880 kb on - strand at 973.936 kb on - strand at 973.938 kb on - strand at 974.060 kb on + strand at 974.060 kb on + strand at 974.093 kb on - strand, within HMPREF1058_RS15210 at 974.130 kb on + strand, within HMPREF1058_RS15210 at 974.133 kb on - strand, within HMPREF1058_RS15210 at 974.156 kb on + strand, within HMPREF1058_RS15210 at 974.187 kb on - strand, within HMPREF1058_RS15210 at 974.225 kb on + strand, within HMPREF1058_RS15210 at 974.225 kb on + strand, within HMPREF1058_RS15210 at 974.226 kb on - strand, within HMPREF1058_RS15210 at 974.226 kb on - strand, within HMPREF1058_RS15210 at 974.226 kb on - strand, within HMPREF1058_RS15210 at 974.265 kb on + strand, within HMPREF1058_RS15210 at 974.265 kb on + strand, within HMPREF1058_RS15210 at 974.265 kb on + strand, within HMPREF1058_RS15210 at 974.331 kb on - strand, within HMPREF1058_RS15210 at 974.331 kb on - strand, within HMPREF1058_RS15210 at 974.332 kb on + strand, within HMPREF1058_RS15210 at 974.339 kb on - strand, within HMPREF1058_RS15210 at 974.339 kb on - strand, within HMPREF1058_RS15210 at 974.344 kb on - strand, within HMPREF1058_RS15210 at 974.346 kb on - strand, within HMPREF1058_RS15210 at 974.402 kb on - strand, within HMPREF1058_RS15210 at 974.402 kb on - strand, within HMPREF1058_RS15210 at 974.402 kb on - strand, within HMPREF1058_RS15210 at 974.487 kb on + strand, within HMPREF1058_RS15210 at 974.620 kb on + strand at 974.621 kb on - strand at 974.679 kb on + strand at 974.680 kb on - strand at 974.681 kb on + strand at 974.685 kb on - strand at 974.771 kb on + strand, within HMPREF1058_RS15205 at 974.868 kb on + strand, within HMPREF1058_RS15205 at 974.869 kb on - strand, within HMPREF1058_RS15205 at 974.872 kb on - strand, within HMPREF1058_RS15205 at 974.878 kb on + strand, within HMPREF1058_RS15205 at 974.879 kb on - strand, within HMPREF1058_RS15205 at 974.880 kb on + strand, within HMPREF1058_RS15205 at 974.914 kb on + strand, within HMPREF1058_RS15205 at 974.915 kb on - strand, within HMPREF1058_RS15205 at 974.915 kb on - strand, within HMPREF1058_RS15205 at 974.937 kb on + strand, within HMPREF1058_RS15205 at 975.031 kb on + strand, within HMPREF1058_RS15205 at 975.032 kb on - strand, within HMPREF1058_RS15205 at 975.039 kb on + strand, within HMPREF1058_RS15205 at 975.090 kb on + strand, within HMPREF1058_RS15205 at 975.148 kb on + strand, within HMPREF1058_RS15205 at 975.150 kb on + strand, within HMPREF1058_RS15205 at 975.205 kb on - strand, within HMPREF1058_RS15205 at 975.205 kb on - strand, within HMPREF1058_RS15205 at 975.249 kb on + strand, within HMPREF1058_RS15205 at 975.250 kb on - strand, within HMPREF1058_RS15205 at 975.287 kb on + strand, within HMPREF1058_RS15205 at 975.461 kb on - strand, within HMPREF1058_RS15205 at 975.496 kb on + strand at 975.497 kb on - strand at 975.502 kb on - strand at 975.549 kb on + strand at 975.564 kb on + strand at 975.566 kb on + strand at 975.567 kb on - strand at 975.567 kb on - strand at 975.580 kb on + strand at 975.599 kb on + strand at 975.650 kb on - strand at 975.655 kb on - strand at 975.693 kb on + strand at 975.702 kb on + strand at 975.717 kb on - strand at 975.756 kb on + strand at 975.782 kb on + strand at 975.888 kb on + strand at 975.899 kb on + strand at 975.998 kb on + strand at 976.007 kb on - strand at 976.007 kb on - strand at 976.040 kb on + strand at 976.040 kb on + strand at 976.040 kb on + strand at 976.041 kb on - strand at 976.043 kb on + strand at 976.067 kb on - strand at 976.082 kb on + strand at 976.168 kb on + strand, within HMPREF1058_RS15200 at 976.224 kb on - strand, within HMPREF1058_RS15200 at 976.316 kb on + strand, within HMPREF1058_RS15200 at 976.316 kb on + strand, within HMPREF1058_RS15200 at 976.316 kb on + strand, within HMPREF1058_RS15200 at 976.316 kb on + strand, within HMPREF1058_RS15200 at 976.317 kb on - strand, within HMPREF1058_RS15200 at 976.318 kb on + strand, within HMPREF1058_RS15200 at 976.318 kb on + strand, within HMPREF1058_RS15200 at 976.321 kb on + strand, within HMPREF1058_RS15200 at 976.339 kb on + strand, within HMPREF1058_RS15200 at 976.405 kb on + strand, within HMPREF1058_RS15200 at 976.406 kb on - strand, within HMPREF1058_RS15200 at 976.408 kb on + strand, within HMPREF1058_RS15200 at 976.409 kb on - strand, within HMPREF1058_RS15200 at 976.409 kb on - strand, within HMPREF1058_RS15200 at 976.454 kb on + strand, within HMPREF1058_RS15200 at 976.467 kb on - strand, within HMPREF1058_RS15200 at 976.521 kb on - strand, within HMPREF1058_RS15200 at 976.562 kb on + strand, within HMPREF1058_RS15200 at 976.564 kb on + strand, within HMPREF1058_RS15200
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 973,618 - HMPREF1058_RS15215 0.73 -2.9 973,647 + HMPREF1058_RS15215 0.76 -1.7 973,678 - HMPREF1058_RS15215 0.78 -0.9 973,718 + HMPREF1058_RS15215 0.82 +0.2 973,752 + HMPREF1058_RS15215 0.85 -2.8 973,752 + HMPREF1058_RS15215 0.85 -2.4 973,753 - HMPREF1058_RS15215 0.85 -1.4 973,755 + HMPREF1058_RS15215 0.85 -1.5 973,843 - -1.2 973,843 - -1.6 973,880 - -0.4 973,936 - -0.8 973,938 - -0.4 974,060 + +0.1 974,060 + -0.5 974,093 - HMPREF1058_RS15210 0.13 +0.6 974,130 + HMPREF1058_RS15210 0.19 -0.3 974,133 - HMPREF1058_RS15210 0.20 +0.9 974,156 + HMPREF1058_RS15210 0.24 +0.5 974,187 - HMPREF1058_RS15210 0.29 +0.6 974,225 + HMPREF1058_RS15210 0.35 -1.3 974,225 + HMPREF1058_RS15210 0.35 +0.7 974,226 - HMPREF1058_RS15210 0.35 +0.5 974,226 - HMPREF1058_RS15210 0.35 -0.3 974,226 - HMPREF1058_RS15210 0.35 +1.1 974,265 + HMPREF1058_RS15210 0.42 -1.3 974,265 + HMPREF1058_RS15210 0.42 -0.1 974,265 + HMPREF1058_RS15210 0.42 -0.3 974,331 - HMPREF1058_RS15210 0.53 +0.6 974,331 - HMPREF1058_RS15210 0.53 +1.1 974,332 + HMPREF1058_RS15210 0.53 +0.4 974,339 - HMPREF1058_RS15210 0.54 +2.3 974,339 - HMPREF1058_RS15210 0.54 +1.1 974,344 - HMPREF1058_RS15210 0.55 +0.4 974,346 - HMPREF1058_RS15210 0.56 +0.8 974,402 - HMPREF1058_RS15210 0.65 +0.2 974,402 - HMPREF1058_RS15210 0.65 +0.7 974,402 - HMPREF1058_RS15210 0.65 +1.4 974,487 + HMPREF1058_RS15210 0.79 -1.7 974,620 + -0.4 974,621 - -1.0 974,679 + -0.4 974,680 - +2.5 974,681 + -0.4 974,685 - +1.9 974,771 + HMPREF1058_RS15205 0.18 +0.9 974,868 + HMPREF1058_RS15205 0.28 +1.5 974,869 - HMPREF1058_RS15205 0.28 +1.3 974,872 - HMPREF1058_RS15205 0.28 +1.2 974,878 + HMPREF1058_RS15205 0.29 -0.1 974,879 - HMPREF1058_RS15205 0.29 +1.4 974,880 + HMPREF1058_RS15205 0.29 -0.5 974,914 + HMPREF1058_RS15205 0.33 +1.4 974,915 - HMPREF1058_RS15205 0.33 +1.5 974,915 - HMPREF1058_RS15205 0.33 +0.4 974,937 + HMPREF1058_RS15205 0.35 +0.9 975,031 + HMPREF1058_RS15205 0.45 -0.0 975,032 - HMPREF1058_RS15205 0.45 +0.9 975,039 + HMPREF1058_RS15205 0.45 +1.2 975,090 + HMPREF1058_RS15205 0.51 -0.2 975,148 + HMPREF1058_RS15205 0.56 -0.7 975,150 + HMPREF1058_RS15205 0.57 -1.5 975,205 - HMPREF1058_RS15205 0.62 +0.9 975,205 - HMPREF1058_RS15205 0.62 +1.3 975,249 + HMPREF1058_RS15205 0.67 -0.7 975,250 - HMPREF1058_RS15205 0.67 +1.3 975,287 + HMPREF1058_RS15205 0.71 -0.3 975,461 - HMPREF1058_RS15205 0.88 +0.7 975,496 + +0.3 975,497 - +1.0 975,502 - -0.2 975,549 + +1.1 975,564 + +0.4 975,566 + +1.0 975,567 - -0.5 975,567 - +0.0 975,580 + -1.3 975,599 + -0.1 975,650 - +1.6 975,655 - +1.0 975,693 + +0.6 975,702 + -0.6 975,717 - +1.7 975,756 + -0.5 975,782 + +0.1 975,888 + -0.1 975,899 + -1.2 975,998 + +0.5 976,007 - -1.4 976,007 - -0.6 976,040 + +0.7 976,040 + -0.7 976,040 + -0.3 976,041 - -0.3 976,043 + +0.2 976,067 - -1.0 976,082 + +0.3 976,168 + HMPREF1058_RS15200 0.12 -0.5 976,224 - HMPREF1058_RS15200 0.15 +0.8 976,316 + HMPREF1058_RS15200 0.20 +0.2 976,316 + HMPREF1058_RS15200 0.20 -0.9 976,316 + HMPREF1058_RS15200 0.20 -0.2 976,316 + HMPREF1058_RS15200 0.20 -0.0 976,317 - HMPREF1058_RS15200 0.20 +0.4 976,318 + HMPREF1058_RS15200 0.20 -0.5 976,318 + HMPREF1058_RS15200 0.20 -0.5 976,321 + HMPREF1058_RS15200 0.20 -0.4 976,339 + HMPREF1058_RS15200 0.21 -0.4 976,405 + HMPREF1058_RS15200 0.24 -1.0 976,406 - HMPREF1058_RS15200 0.24 +0.3 976,408 + HMPREF1058_RS15200 0.24 +0.5 976,409 - HMPREF1058_RS15200 0.24 -0.0 976,409 - HMPREF1058_RS15200 0.24 +0.2 976,454 + HMPREF1058_RS15200 0.26 +0.0 976,467 - HMPREF1058_RS15200 0.27 -0.4 976,521 - HMPREF1058_RS15200 0.30 -0.0 976,562 + HMPREF1058_RS15200 0.32 +0.9 976,564 + HMPREF1058_RS15200 0.32 -1.6
Or see this region's nucleotide sequence