Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS14305 and HMPREF1058_RS14300 are separated by 136 nucleotides HMPREF1058_RS14300 and HMPREF1058_RS14295 are separated by 131 nucleotides HMPREF1058_RS14295 and HMPREF1058_RS14290 are separated by 2 nucleotides
HMPREF1058_RS14305: HMPREF1058_RS14305 - DNA topoisomerase 3, at 1,240,107 to 1,242,248
_RS14305
HMPREF1058_RS14300: HMPREF1058_RS14300 - rRNA cytosine-C5-methyltransferase, at 1,242,385 to 1,243,812
_RS14300
HMPREF1058_RS14295: HMPREF1058_RS14295 - hypothetical protein, at 1,243,944 to 1,244,651
_RS14295
HMPREF1058_RS14290: HMPREF1058_RS14290 - sigma-70 family RNA polymerase sigma factor, at 1,244,654 to 1,245,202
_RS14290
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1241.822 kb on - strand, within HMPREF1058_RS14305 at 1241.966 kb on - strand, within HMPREF1058_RS14305 at 1241.990 kb on + strand, within HMPREF1058_RS14305 at 1242.228 kb on - strand at 1242.287 kb on - strand at 1242.288 kb on + strand at 1242.289 kb on - strand at 1242.293 kb on + strand at 1242.298 kb on + strand at 1242.337 kb on + strand at 1242.349 kb on + strand at 1242.350 kb on - strand at 1242.375 kb on - strand at 1242.392 kb on - strand at 1242.417 kb on - strand at 1242.515 kb on - strand at 1242.515 kb on - strand at 1242.519 kb on - strand at 1242.567 kb on - strand, within HMPREF1058_RS14300 at 1242.653 kb on - strand, within HMPREF1058_RS14300 at 1242.679 kb on + strand, within HMPREF1058_RS14300 at 1242.679 kb on + strand, within HMPREF1058_RS14300 at 1242.681 kb on + strand, within HMPREF1058_RS14300 at 1242.681 kb on + strand, within HMPREF1058_RS14300 at 1242.706 kb on + strand, within HMPREF1058_RS14300 at 1242.728 kb on + strand, within HMPREF1058_RS14300 at 1242.729 kb on - strand, within HMPREF1058_RS14300 at 1242.952 kb on + strand, within HMPREF1058_RS14300 at 1243.069 kb on + strand, within HMPREF1058_RS14300 at 1243.128 kb on + strand, within HMPREF1058_RS14300 at 1243.130 kb on + strand, within HMPREF1058_RS14300 at 1243.146 kb on - strand, within HMPREF1058_RS14300 at 1243.183 kb on - strand, within HMPREF1058_RS14300 at 1243.446 kb on + strand, within HMPREF1058_RS14300 at 1243.531 kb on - strand, within HMPREF1058_RS14300 at 1243.581 kb on - strand, within HMPREF1058_RS14300 at 1243.581 kb on - strand, within HMPREF1058_RS14300 at 1243.583 kb on + strand, within HMPREF1058_RS14300 at 1243.583 kb on + strand, within HMPREF1058_RS14300 at 1243.615 kb on - strand, within HMPREF1058_RS14300 at 1243.634 kb on - strand, within HMPREF1058_RS14300 at 1243.638 kb on - strand, within HMPREF1058_RS14300 at 1243.804 kb on + strand at 1243.888 kb on + strand at 1243.889 kb on - strand at 1243.913 kb on + strand at 1243.913 kb on + strand at 1243.926 kb on + strand at 1244.017 kb on + strand, within HMPREF1058_RS14295 at 1244.020 kb on + strand, within HMPREF1058_RS14295 at 1244.062 kb on + strand, within HMPREF1058_RS14295 at 1244.114 kb on + strand, within HMPREF1058_RS14295 at 1244.210 kb on + strand, within HMPREF1058_RS14295 at 1244.211 kb on - strand, within HMPREF1058_RS14295 at 1244.216 kb on - strand, within HMPREF1058_RS14295 at 1244.230 kb on - strand, within HMPREF1058_RS14295 at 1244.230 kb on - strand, within HMPREF1058_RS14295 at 1244.251 kb on - strand, within HMPREF1058_RS14295 at 1244.357 kb on + strand, within HMPREF1058_RS14295 at 1244.358 kb on - strand, within HMPREF1058_RS14295 at 1244.359 kb on + strand, within HMPREF1058_RS14295 at 1244.360 kb on + strand, within HMPREF1058_RS14295 at 1244.462 kb on - strand, within HMPREF1058_RS14295 at 1244.472 kb on + strand, within HMPREF1058_RS14295 at 1244.614 kb on + strand at 1244.650 kb on - strand at 1244.702 kb on - strand at 1244.738 kb on + strand, within HMPREF1058_RS14290 at 1244.785 kb on + strand, within HMPREF1058_RS14290
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 1,241,822 - HMPREF1058_RS14305 0.80 +0.2 1,241,966 - HMPREF1058_RS14305 0.87 +0.6 1,241,990 + HMPREF1058_RS14305 0.88 -1.3 1,242,228 - -2.2 1,242,287 - -0.4 1,242,288 + +0.8 1,242,289 - -1.1 1,242,293 + -3.3 1,242,298 + +0.6 1,242,337 + +0.8 1,242,349 + -0.0 1,242,350 - +0.1 1,242,375 - -0.4 1,242,392 - -1.0 1,242,417 - +0.4 1,242,515 - +1.0 1,242,515 - +1.4 1,242,519 - +1.8 1,242,567 - HMPREF1058_RS14300 0.13 +1.2 1,242,653 - HMPREF1058_RS14300 0.19 +0.3 1,242,679 + HMPREF1058_RS14300 0.21 +1.0 1,242,679 + HMPREF1058_RS14300 0.21 +1.9 1,242,681 + HMPREF1058_RS14300 0.21 +0.6 1,242,681 + HMPREF1058_RS14300 0.21 +0.5 1,242,706 + HMPREF1058_RS14300 0.22 +1.2 1,242,728 + HMPREF1058_RS14300 0.24 +1.0 1,242,729 - HMPREF1058_RS14300 0.24 +0.8 1,242,952 + HMPREF1058_RS14300 0.40 +1.0 1,243,069 + HMPREF1058_RS14300 0.48 -0.1 1,243,128 + HMPREF1058_RS14300 0.52 +0.3 1,243,130 + HMPREF1058_RS14300 0.52 +2.5 1,243,146 - HMPREF1058_RS14300 0.53 -0.8 1,243,183 - HMPREF1058_RS14300 0.56 -0.9 1,243,446 + HMPREF1058_RS14300 0.74 +1.1 1,243,531 - HMPREF1058_RS14300 0.80 +1.8 1,243,581 - HMPREF1058_RS14300 0.84 +0.1 1,243,581 - HMPREF1058_RS14300 0.84 -0.1 1,243,583 + HMPREF1058_RS14300 0.84 +1.6 1,243,583 + HMPREF1058_RS14300 0.84 +0.6 1,243,615 - HMPREF1058_RS14300 0.86 -0.8 1,243,634 - HMPREF1058_RS14300 0.87 +1.0 1,243,638 - HMPREF1058_RS14300 0.88 +2.0 1,243,804 + +1.3 1,243,888 + +0.2 1,243,889 - -0.3 1,243,913 + +0.4 1,243,913 + +0.6 1,243,926 + -0.4 1,244,017 + HMPREF1058_RS14295 0.10 +0.4 1,244,020 + HMPREF1058_RS14295 0.11 -0.7 1,244,062 + HMPREF1058_RS14295 0.17 +2.0 1,244,114 + HMPREF1058_RS14295 0.24 +0.5 1,244,210 + HMPREF1058_RS14295 0.38 -0.6 1,244,211 - HMPREF1058_RS14295 0.38 -1.1 1,244,216 - HMPREF1058_RS14295 0.38 -1.1 1,244,230 - HMPREF1058_RS14295 0.40 +0.4 1,244,230 - HMPREF1058_RS14295 0.40 +0.8 1,244,251 - HMPREF1058_RS14295 0.43 -0.5 1,244,357 + HMPREF1058_RS14295 0.58 -0.8 1,244,358 - HMPREF1058_RS14295 0.58 +1.2 1,244,359 + HMPREF1058_RS14295 0.59 -2.1 1,244,360 + HMPREF1058_RS14295 0.59 -1.0 1,244,462 - HMPREF1058_RS14295 0.73 -1.5 1,244,472 + HMPREF1058_RS14295 0.75 +0.6 1,244,614 + +0.9 1,244,650 - -0.3 1,244,702 - -1.3 1,244,738 + HMPREF1058_RS14290 0.15 -0.1 1,244,785 + HMPREF1058_RS14290 0.24 -0.3
Or see this region's nucleotide sequence