Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS14225 and HMPREF1058_RS14220 are separated by 81 nucleotides HMPREF1058_RS14220 and HMPREF1058_RS14215 are separated by 185 nucleotides HMPREF1058_RS14215 and HMPREF1058_RS14210 overlap by 8 nucleotides
HMPREF1058_RS14225: HMPREF1058_RS14225 - polyprenyl synthetase family protein, at 1,253,074 to 1,254,051
_RS14225
HMPREF1058_RS14220: HMPREF1058_RS14220 - energy transducer TonB, at 1,254,133 to 1,254,816
_RS14220
HMPREF1058_RS14215: HMPREF1058_RS14215 - (d)CMP kinase, at 1,255,002 to 1,255,691
_RS14215
HMPREF1058_RS14210: HMPREF1058_RS14210 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 1,255,684 to 1,256,550
_RS14210
Position (kb)
1254
1255 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1253.145 kb on + strand at 1253.172 kb on + strand, within HMPREF1058_RS14225 at 1253.221 kb on - strand, within HMPREF1058_RS14225 at 1253.272 kb on - strand, within HMPREF1058_RS14225 at 1253.375 kb on - strand, within HMPREF1058_RS14225 at 1253.405 kb on + strand, within HMPREF1058_RS14225 at 1253.467 kb on + strand, within HMPREF1058_RS14225 at 1253.467 kb on + strand, within HMPREF1058_RS14225 at 1253.468 kb on - strand, within HMPREF1058_RS14225 at 1253.503 kb on + strand, within HMPREF1058_RS14225 at 1253.655 kb on + strand, within HMPREF1058_RS14225 at 1253.676 kb on - strand, within HMPREF1058_RS14225 at 1253.676 kb on - strand, within HMPREF1058_RS14225 at 1253.773 kb on - strand, within HMPREF1058_RS14225 at 1253.827 kb on - strand, within HMPREF1058_RS14225 at 1253.827 kb on - strand, within HMPREF1058_RS14225 at 1253.833 kb on - strand, within HMPREF1058_RS14225 at 1253.846 kb on - strand, within HMPREF1058_RS14225 at 1253.846 kb on - strand, within HMPREF1058_RS14225 at 1253.846 kb on - strand, within HMPREF1058_RS14225 at 1253.952 kb on - strand, within HMPREF1058_RS14225 at 1253.952 kb on - strand, within HMPREF1058_RS14225 at 1253.956 kb on + strand at 1253.967 kb on - strand at 1254.013 kb on - strand at 1254.013 kb on - strand at 1254.013 kb on - strand at 1254.114 kb on - strand at 1254.114 kb on - strand at 1254.118 kb on + strand at 1254.118 kb on + strand at 1254.162 kb on + strand at 1254.176 kb on - strand at 1254.260 kb on - strand, within HMPREF1058_RS14220 at 1254.301 kb on - strand, within HMPREF1058_RS14220 at 1254.328 kb on - strand, within HMPREF1058_RS14220 at 1254.367 kb on - strand, within HMPREF1058_RS14220 at 1254.488 kb on + strand, within HMPREF1058_RS14220 at 1254.549 kb on + strand, within HMPREF1058_RS14220 at 1254.550 kb on - strand, within HMPREF1058_RS14220 at 1254.584 kb on - strand, within HMPREF1058_RS14220 at 1254.589 kb on - strand, within HMPREF1058_RS14220 at 1254.658 kb on + strand, within HMPREF1058_RS14220 at 1254.673 kb on - strand at 1254.750 kb on - strand at 1254.816 kb on + strand at 1254.860 kb on + strand at 1254.941 kb on + strand at 1254.941 kb on + strand at 1254.988 kb on + strand at 1254.997 kb on + strand at 1254.997 kb on + strand at 1254.999 kb on + strand at 1254.999 kb on + strand at 1255.092 kb on + strand, within HMPREF1058_RS14215 at 1255.129 kb on + strand, within HMPREF1058_RS14215 at 1255.131 kb on + strand, within HMPREF1058_RS14215 at 1255.133 kb on + strand, within HMPREF1058_RS14215 at 1255.133 kb on + strand, within HMPREF1058_RS14215 at 1255.133 kb on + strand, within HMPREF1058_RS14215 at 1255.208 kb on + strand, within HMPREF1058_RS14215 at 1255.208 kb on + strand, within HMPREF1058_RS14215 at 1255.521 kb on + strand, within HMPREF1058_RS14215 at 1255.694 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 1,253,145 + -1.4 1,253,172 + HMPREF1058_RS14225 0.10 -2.0 1,253,221 - HMPREF1058_RS14225 0.15 -0.8 1,253,272 - HMPREF1058_RS14225 0.20 -0.2 1,253,375 - HMPREF1058_RS14225 0.31 -0.6 1,253,405 + HMPREF1058_RS14225 0.34 -1.2 1,253,467 + HMPREF1058_RS14225 0.40 +0.4 1,253,467 + HMPREF1058_RS14225 0.40 -1.5 1,253,468 - HMPREF1058_RS14225 0.40 -1.3 1,253,503 + HMPREF1058_RS14225 0.44 +0.2 1,253,655 + HMPREF1058_RS14225 0.59 -1.5 1,253,676 - HMPREF1058_RS14225 0.62 -2.3 1,253,676 - HMPREF1058_RS14225 0.62 -2.6 1,253,773 - HMPREF1058_RS14225 0.71 -2.8 1,253,827 - HMPREF1058_RS14225 0.77 -0.8 1,253,827 - HMPREF1058_RS14225 0.77 -2.7 1,253,833 - HMPREF1058_RS14225 0.78 -2.2 1,253,846 - HMPREF1058_RS14225 0.79 -2.5 1,253,846 - HMPREF1058_RS14225 0.79 -2.1 1,253,846 - HMPREF1058_RS14225 0.79 -0.6 1,253,952 - HMPREF1058_RS14225 0.90 -1.1 1,253,952 - HMPREF1058_RS14225 0.90 -1.3 1,253,956 + -1.4 1,253,967 - +0.2 1,254,013 - -1.4 1,254,013 - +0.6 1,254,013 - -2.2 1,254,114 - +0.1 1,254,114 - -0.4 1,254,118 + +1.4 1,254,118 + -0.8 1,254,162 + +1.8 1,254,176 - +2.0 1,254,260 - HMPREF1058_RS14220 0.19 +2.1 1,254,301 - HMPREF1058_RS14220 0.25 +2.5 1,254,328 - HMPREF1058_RS14220 0.29 +3.0 1,254,367 - HMPREF1058_RS14220 0.34 +2.6 1,254,488 + HMPREF1058_RS14220 0.52 +0.2 1,254,549 + HMPREF1058_RS14220 0.61 -0.3 1,254,550 - HMPREF1058_RS14220 0.61 +3.2 1,254,584 - HMPREF1058_RS14220 0.66 +3.4 1,254,589 - HMPREF1058_RS14220 0.67 +3.3 1,254,658 + HMPREF1058_RS14220 0.77 +0.5 1,254,673 - -0.8 1,254,750 - +2.1 1,254,816 + +2.3 1,254,860 + +1.5 1,254,941 + +0.5 1,254,941 + +0.1 1,254,988 + -1.4 1,254,997 + +0.0 1,254,997 + -3.2 1,254,999 + -1.1 1,254,999 + -0.6 1,255,092 + HMPREF1058_RS14215 0.13 +0.4 1,255,129 + HMPREF1058_RS14215 0.18 -0.2 1,255,131 + HMPREF1058_RS14215 0.19 -0.1 1,255,133 + HMPREF1058_RS14215 0.19 -0.4 1,255,133 + HMPREF1058_RS14215 0.19 -1.1 1,255,133 + HMPREF1058_RS14215 0.19 -0.1 1,255,208 + HMPREF1058_RS14215 0.30 -3.1 1,255,208 + HMPREF1058_RS14215 0.30 -2.1 1,255,521 + HMPREF1058_RS14215 0.75 +0.8 1,255,694 + -0.2
Or see this region's nucleotide sequence