Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05790 and HMPREF1058_RS21630 are separated by 172 nucleotides HMPREF1058_RS21630 and HMPREF1058_RS05785 are separated by 344 nucleotides
HMPREF1058_RS05790: HMPREF1058_RS05790 - hypothetical protein, at 79,522 to 79,743
_RS05790
HMPREF1058_RS21630: HMPREF1058_RS21630 - hypothetical protein, at 79,916 to 80,092
_RS21630
HMPREF1058_RS05785: HMPREF1058_RS05785 - adenine nucleotide alpha hydrolase family protein, at 80,437 to 81,192
_RS05785
Position (kb) 79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 78.691 kb on + strand at 78.696 kb on + strand at 78.728 kb on + strand at 78.728 kb on + strand at 78.730 kb on + strand at 78.730 kb on + strand at 78.731 kb on - strand at 78.731 kb on - strand at 78.734 kb on + strand at 78.760 kb on + strand at 78.761 kb on - strand at 78.761 kb on - strand at 78.766 kb on + strand at 78.766 kb on + strand at 79.087 kb on - strand at 79.118 kb on - strand at 79.134 kb on + strand at 79.135 kb on - strand at 79.458 kb on + strand at 79.487 kb on - strand at 79.517 kb on - strand at 79.596 kb on + strand, within HMPREF1058_RS05790 at 79.597 kb on - strand, within HMPREF1058_RS05790 at 79.656 kb on + strand, within HMPREF1058_RS05790 at 79.742 kb on - strand at 79.744 kb on + strand at 79.745 kb on - strand at 79.748 kb on + strand at 79.821 kb on - strand at 79.823 kb on - strand at 79.836 kb on + strand at 79.842 kb on + strand at 79.843 kb on - strand at 79.964 kb on - strand, within HMPREF1058_RS21630 at 80.021 kb on + strand, within HMPREF1058_RS21630 at 80.021 kb on + strand, within HMPREF1058_RS21630 at 80.022 kb on - strand, within HMPREF1058_RS21630 at 80.023 kb on + strand, within HMPREF1058_RS21630 at 80.023 kb on + strand at 80.024 kb on - strand, within HMPREF1058_RS21630 at 80.024 kb on - strand, within HMPREF1058_RS21630 at 80.024 kb on - strand, within HMPREF1058_RS21630 at 80.051 kb on + strand, within HMPREF1058_RS21630 at 80.070 kb on - strand, within HMPREF1058_RS21630 at 80.098 kb on - strand at 80.177 kb on - strand at 80.197 kb on - strand at 80.267 kb on + strand at 80.268 kb on - strand at 80.268 kb on - strand at 80.315 kb on + strand at 80.315 kb on + strand at 80.333 kb on - strand at 80.337 kb on - strand at 80.348 kb on - strand at 80.387 kb on - strand at 80.391 kb on - strand at 80.392 kb on + strand at 80.407 kb on + strand at 80.441 kb on - strand at 80.506 kb on - strand at 80.506 kb on - strand at 80.540 kb on + strand, within HMPREF1058_RS05785 at 80.542 kb on + strand, within HMPREF1058_RS05785 at 80.557 kb on - strand, within HMPREF1058_RS05785 at 80.671 kb on - strand, within HMPREF1058_RS05785 at 80.715 kb on + strand, within HMPREF1058_RS05785 at 80.739 kb on + strand, within HMPREF1058_RS05785
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 78,691 + +0.3 78,696 + -1.8 78,728 + +0.4 78,728 + +1.0 78,730 + -0.0 78,730 + -1.0 78,731 - +0.0 78,731 - +1.4 78,734 + +0.1 78,760 + +0.7 78,761 - -0.3 78,761 - -0.8 78,766 + -2.8 78,766 + +0.5 79,087 - -0.4 79,118 - +1.4 79,134 + +0.6 79,135 - -0.6 79,458 + +0.9 79,487 - -1.5 79,517 - -1.7 79,596 + HMPREF1058_RS05790 0.33 +1.6 79,597 - HMPREF1058_RS05790 0.34 +1.1 79,656 + HMPREF1058_RS05790 0.60 -0.5 79,742 - -0.4 79,744 + +0.5 79,745 - -0.4 79,748 + +0.4 79,821 - -2.3 79,823 - -2.0 79,836 + +0.0 79,842 + -0.1 79,843 - +1.1 79,964 - HMPREF1058_RS21630 0.27 +0.7 80,021 + HMPREF1058_RS21630 0.59 +0.6 80,021 + HMPREF1058_RS21630 0.59 +1.4 80,022 - HMPREF1058_RS21630 0.60 +0.5 80,023 + HMPREF1058_RS21630 0.60 -0.1 80,023 + +0.4 80,024 - HMPREF1058_RS21630 0.61 -0.3 80,024 - HMPREF1058_RS21630 0.61 -0.1 80,024 - HMPREF1058_RS21630 0.61 -1.0 80,051 + HMPREF1058_RS21630 0.76 +1.5 80,070 - HMPREF1058_RS21630 0.87 +0.3 80,098 - +1.2 80,177 - +0.5 80,197 - -0.8 80,267 + -1.4 80,268 - +1.2 80,268 - -0.3 80,315 + +0.7 80,315 + +0.9 80,333 - -0.3 80,337 - -1.1 80,348 - -0.8 80,387 - +1.5 80,391 - +0.2 80,392 + -0.6 80,407 + -1.5 80,441 - -0.3 80,506 - -1.3 80,506 - +1.0 80,540 + HMPREF1058_RS05785 0.14 -0.1 80,542 + HMPREF1058_RS05785 0.14 -0.3 80,557 - HMPREF1058_RS05785 0.16 -1.6 80,671 - HMPREF1058_RS05785 0.31 -1.0 80,715 + HMPREF1058_RS05785 0.37 +0.8 80,739 + HMPREF1058_RS05785 0.40 +0.3
Or see this region's nucleotide sequence