Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS04720 and HMPREF1058_RS04715 are separated by 195 nucleotides HMPREF1058_RS04715 and HMPREF1058_RS04710 are separated by 180 nucleotides HMPREF1058_RS04710 and HMPREF1058_RS04705 are separated by 24 nucleotides
HMPREF1058_RS04720: HMPREF1058_RS04720 - transcription antitermination factor NusB, at 327,828 to 328,754
_RS04720
HMPREF1058_RS04715: HMPREF1058_RS04715 - PUR family DNA/RNA-binding protein, at 328,950 to 329,333
_RS04715
HMPREF1058_RS04710: HMPREF1058_RS04710 - 50S ribosomal protein L25/general stress protein Ctc, at 329,514 to 330,110
_RS04710
HMPREF1058_RS04705: HMPREF1058_RS04705 - aminoacyl-tRNA hydrolase, at 330,135 to 330,698
_RS04705
Position (kb) 328
329
330 Strain fitness (log2 ratio) -1
0
1
2
3 at 328.817 kb on - strand at 328.845 kb on + strand at 328.851 kb on + strand at 328.856 kb on - strand at 328.961 kb on + strand at 328.961 kb on + strand at 328.976 kb on - strand at 329.030 kb on + strand, within HMPREF1058_RS04715 at 329.042 kb on - strand, within HMPREF1058_RS04715 at 329.131 kb on + strand, within HMPREF1058_RS04715 at 329.206 kb on + strand, within HMPREF1058_RS04715 at 329.207 kb on - strand, within HMPREF1058_RS04715 at 329.235 kb on - strand, within HMPREF1058_RS04715 at 329.312 kb on + strand at 329.439 kb on + strand at 329.482 kb on + strand at 330.108 kb on + strand at 330.123 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 328,817 - +3.4 328,845 + +1.1 328,851 + -1.0 328,856 - -1.5 328,961 + +1.7 328,961 + +1.0 328,976 - -1.3 329,030 + HMPREF1058_RS04715 0.21 +1.0 329,042 - HMPREF1058_RS04715 0.24 -0.2 329,131 + HMPREF1058_RS04715 0.47 +0.1 329,206 + HMPREF1058_RS04715 0.67 +1.3 329,207 - HMPREF1058_RS04715 0.67 -0.0 329,235 - HMPREF1058_RS04715 0.74 -1.3 329,312 + +0.4 329,439 + +0.4 329,482 + -1.0 330,108 + +1.4 330,123 + +1.8
Or see this region's nucleotide sequence