Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS04235 and HMPREF1058_RS04230 are separated by 387 nucleotides HMPREF1058_RS04230 and HMPREF1058_RS04225 are separated by 91 nucleotides
HMPREF1058_RS04235: HMPREF1058_RS04235 - NUDIX hydrolase, at 85,805 to 86,359
_RS04235
HMPREF1058_RS04230: HMPREF1058_RS04230 - pyridoxamine 5'-phosphate oxidase family protein, at 86,747 to 87,166
_RS04230
HMPREF1058_RS04225: HMPREF1058_RS04225 - helix-turn-helix transcriptional regulator, at 87,258 to 88,148
_RS04225
Position (kb) 86
87
88 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 85.764 kb on + strand at 85.771 kb on + strand at 85.772 kb on - strand at 85.773 kb on + strand at 85.778 kb on + strand at 85.779 kb on - strand at 85.779 kb on - strand at 85.786 kb on - strand at 85.802 kb on + strand at 85.805 kb on - strand at 85.847 kb on + strand at 85.847 kb on + strand at 85.896 kb on + strand, within HMPREF1058_RS04235 at 85.896 kb on + strand, within HMPREF1058_RS04235 at 85.925 kb on + strand, within HMPREF1058_RS04235 at 85.927 kb on + strand, within HMPREF1058_RS04235 at 85.951 kb on - strand, within HMPREF1058_RS04235 at 85.956 kb on + strand, within HMPREF1058_RS04235 at 85.990 kb on - strand, within HMPREF1058_RS04235 at 86.346 kb on - strand at 86.357 kb on + strand at 86.358 kb on - strand at 86.358 kb on - strand at 86.360 kb on + strand at 86.366 kb on + strand at 86.366 kb on + strand at 86.367 kb on - strand at 86.367 kb on - strand at 86.367 kb on - strand at 86.367 kb on - strand at 86.368 kb on + strand at 86.368 kb on + strand at 86.368 kb on + strand at 86.369 kb on - strand at 86.410 kb on + strand at 86.459 kb on - strand at 86.472 kb on + strand at 86.816 kb on + strand, within HMPREF1058_RS04230 at 86.823 kb on + strand, within HMPREF1058_RS04230 at 86.853 kb on + strand, within HMPREF1058_RS04230 at 86.993 kb on + strand, within HMPREF1058_RS04230 at 86.994 kb on - strand, within HMPREF1058_RS04230 at 87.052 kb on + strand, within HMPREF1058_RS04230 at 87.053 kb on - strand, within HMPREF1058_RS04230 at 87.080 kb on - strand, within HMPREF1058_RS04230 at 87.232 kb on - strand at 87.378 kb on - strand, within HMPREF1058_RS04225 at 87.385 kb on + strand, within HMPREF1058_RS04225 at 87.431 kb on - strand, within HMPREF1058_RS04225 at 87.488 kb on + strand, within HMPREF1058_RS04225 at 87.488 kb on + strand, within HMPREF1058_RS04225 at 87.489 kb on - strand, within HMPREF1058_RS04225 at 87.657 kb on - strand, within HMPREF1058_RS04225 at 87.736 kb on - strand, within HMPREF1058_RS04225 at 87.758 kb on - strand, within HMPREF1058_RS04225 at 87.777 kb on + strand, within HMPREF1058_RS04225 at 87.893 kb on + strand, within HMPREF1058_RS04225 at 87.894 kb on - strand, within HMPREF1058_RS04225 at 87.899 kb on + strand, within HMPREF1058_RS04225 at 87.961 kb on + strand, within HMPREF1058_RS04225 at 88.011 kb on - strand, within HMPREF1058_RS04225 at 88.018 kb on + strand, within HMPREF1058_RS04225 at 88.021 kb on + strand, within HMPREF1058_RS04225
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 85,764 + -1.0 85,771 + -0.3 85,772 - +0.6 85,773 + -0.0 85,778 + -0.5 85,779 - -0.4 85,779 - +0.6 85,786 - +0.4 85,802 + -1.1 85,805 - -0.5 85,847 + -2.2 85,847 + +0.3 85,896 + HMPREF1058_RS04235 0.16 -0.1 85,896 + HMPREF1058_RS04235 0.16 -0.6 85,925 + HMPREF1058_RS04235 0.22 -2.0 85,927 + HMPREF1058_RS04235 0.22 -0.1 85,951 - HMPREF1058_RS04235 0.26 +0.8 85,956 + HMPREF1058_RS04235 0.27 -0.2 85,990 - HMPREF1058_RS04235 0.33 +0.9 86,346 - -0.5 86,357 + -0.4 86,358 - +0.4 86,358 - +1.2 86,360 + +0.4 86,366 + -0.9 86,366 + +0.2 86,367 - +0.2 86,367 - -0.2 86,367 - +0.5 86,367 - -2.3 86,368 + +1.1 86,368 + +0.2 86,368 + -1.4 86,369 - +1.4 86,410 + +0.1 86,459 - +1.9 86,472 + +1.3 86,816 + HMPREF1058_RS04230 0.16 -0.5 86,823 + HMPREF1058_RS04230 0.18 +0.7 86,853 + HMPREF1058_RS04230 0.25 +0.0 86,993 + HMPREF1058_RS04230 0.59 +1.4 86,994 - HMPREF1058_RS04230 0.59 +0.9 87,052 + HMPREF1058_RS04230 0.73 +1.1 87,053 - HMPREF1058_RS04230 0.73 -0.1 87,080 - HMPREF1058_RS04230 0.79 -0.9 87,232 - +0.6 87,378 - HMPREF1058_RS04225 0.13 +0.3 87,385 + HMPREF1058_RS04225 0.14 -1.3 87,431 - HMPREF1058_RS04225 0.19 -1.1 87,488 + HMPREF1058_RS04225 0.26 -0.6 87,488 + HMPREF1058_RS04225 0.26 +1.1 87,489 - HMPREF1058_RS04225 0.26 -0.8 87,657 - HMPREF1058_RS04225 0.45 -1.4 87,736 - HMPREF1058_RS04225 0.54 -0.5 87,758 - HMPREF1058_RS04225 0.56 +0.2 87,777 + HMPREF1058_RS04225 0.58 +0.2 87,893 + HMPREF1058_RS04225 0.71 +1.5 87,894 - HMPREF1058_RS04225 0.71 +0.9 87,899 + HMPREF1058_RS04225 0.72 +0.2 87,961 + HMPREF1058_RS04225 0.79 +3.4 88,011 - HMPREF1058_RS04225 0.85 -0.8 88,018 + HMPREF1058_RS04225 0.85 +1.4 88,021 + HMPREF1058_RS04225 0.86 -0.2
Or see this region's nucleotide sequence