Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS21110 and HMPREF1058_RS03435 are separated by 286 nucleotides HMPREF1058_RS03435 and HMPREF1058_RS21670 are separated by 74 nucleotides HMPREF1058_RS21670 and HMPREF1058_RS03430 overlap by 4 nucleotides HMPREF1058_RS03430 and HMPREF1058_RS03425 are separated by 340 nucleotides
HMPREF1058_RS21110: HMPREF1058_RS21110 - DUF4248 domain-containing protein, at 280,870 to 281,076
_RS21110
HMPREF1058_RS03435: HMPREF1058_RS03435 - DNA-binding protein, at 281,363 to 281,869
_RS03435
HMPREF1058_RS21670: HMPREF1058_RS21670 - smalltalk protein, at 281,944 to 282,063
_RS21670
HMPREF1058_RS03430: HMPREF1058_RS03430 - N-acetylmuramoyl-L-alanine amidase, at 282,060 to 282,572
_RS03430
HMPREF1058_RS03425: HMPREF1058_RS03425 - glycosyltransferase family 4 protein, at 282,913 to 284,058
_RS03425
Position (kb)
282
283 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 281.112 kb on + strand at 281.122 kb on + strand at 281.188 kb on + strand at 281.210 kb on + strand at 281.210 kb on + strand at 281.211 kb on - strand at 281.348 kb on - strand at 281.380 kb on + strand at 281.380 kb on + strand at 281.444 kb on + strand, within HMPREF1058_RS03435 at 281.504 kb on + strand, within HMPREF1058_RS03435 at 281.522 kb on + strand, within HMPREF1058_RS03435 at 281.523 kb on - strand, within HMPREF1058_RS03435 at 281.665 kb on - strand at 282.147 kb on + strand, within HMPREF1058_RS03430 at 282.147 kb on + strand, within HMPREF1058_RS03430 at 282.653 kb on - strand at 282.653 kb on - strand at 282.681 kb on + strand at 282.857 kb on - strand at 282.901 kb on + strand at 282.901 kb on + strand at 282.901 kb on + strand at 282.915 kb on - strand at 282.915 kb on - strand at 282.942 kb on - strand at 283.056 kb on + strand, within HMPREF1058_RS03425 at 283.057 kb on - strand, within HMPREF1058_RS03425 at 283.057 kb on - strand, within HMPREF1058_RS03425 at 283.157 kb on + strand, within HMPREF1058_RS03425 at 283.220 kb on + strand, within HMPREF1058_RS03425 at 283.221 kb on - strand, within HMPREF1058_RS03425 at 283.221 kb on - strand, within HMPREF1058_RS03425 at 283.230 kb on + strand, within HMPREF1058_RS03425 at 283.299 kb on + strand, within HMPREF1058_RS03425 at 283.315 kb on - strand, within HMPREF1058_RS03425 at 283.334 kb on - strand, within HMPREF1058_RS03425 at 283.489 kb on - strand, within HMPREF1058_RS03425 at 283.492 kb on - strand at 283.511 kb on - strand, within HMPREF1058_RS03425 at 283.549 kb on - strand, within HMPREF1058_RS03425 at 283.549 kb on - strand, within HMPREF1058_RS03425 at 283.551 kb on + strand, within HMPREF1058_RS03425
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 281,112 + -0.8 281,122 + +0.3 281,188 + -1.3 281,210 + -0.2 281,210 + -2.1 281,211 - +0.4 281,348 - +2.4 281,380 + +1.0 281,380 + -1.0 281,444 + HMPREF1058_RS03435 0.16 -0.3 281,504 + HMPREF1058_RS03435 0.28 +0.5 281,522 + HMPREF1058_RS03435 0.31 -0.3 281,523 - HMPREF1058_RS03435 0.32 +0.8 281,665 - +1.5 282,147 + HMPREF1058_RS03430 0.17 +1.3 282,147 + HMPREF1058_RS03430 0.17 +1.1 282,653 - -0.4 282,653 - -1.0 282,681 + -2.6 282,857 - -0.5 282,901 + +0.3 282,901 + -2.5 282,901 + +0.5 282,915 - +0.1 282,915 - +0.2 282,942 - -0.6 283,056 + HMPREF1058_RS03425 0.12 -0.8 283,057 - HMPREF1058_RS03425 0.13 -0.5 283,057 - HMPREF1058_RS03425 0.13 -1.9 283,157 + HMPREF1058_RS03425 0.21 -1.0 283,220 + HMPREF1058_RS03425 0.27 -1.9 283,221 - HMPREF1058_RS03425 0.27 +0.8 283,221 - HMPREF1058_RS03425 0.27 -0.5 283,230 + HMPREF1058_RS03425 0.28 -0.5 283,299 + HMPREF1058_RS03425 0.34 -0.6 283,315 - HMPREF1058_RS03425 0.35 +0.2 283,334 - HMPREF1058_RS03425 0.37 +0.9 283,489 - HMPREF1058_RS03425 0.50 +0.0 283,492 - +1.8 283,511 - HMPREF1058_RS03425 0.52 +0.3 283,549 - HMPREF1058_RS03425 0.55 +0.4 283,549 - HMPREF1058_RS03425 0.55 -1.3 283,551 + HMPREF1058_RS03425 0.56 -1.8
Or see this region's nucleotide sequence