Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS02835 and HMPREF1058_RS02830 overlap by 4 nucleotides HMPREF1058_RS02830 and HMPREF1058_RS02825 are separated by 98 nucleotides
HMPREF1058_RS02835: HMPREF1058_RS02835 - family 20 glycosylhydrolase, at 78,965 to 81,046
_RS02835
HMPREF1058_RS02830: HMPREF1058_RS02830 - DUF4982 domain-containing protein, at 81,043 to 83,490
_RS02830
HMPREF1058_RS02825: HMPREF1058_RS02825 - RagB/SusD family nutrient uptake outer membrane protein, at 83,589 to 85,130
_RS02825
Position (kb) 81
82
83
84 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 80.060 kb on - strand, within HMPREF1058_RS02835 at 80.060 kb on - strand, within HMPREF1058_RS02835 at 80.161 kb on + strand, within HMPREF1058_RS02835 at 80.204 kb on + strand, within HMPREF1058_RS02835 at 80.206 kb on + strand, within HMPREF1058_RS02835 at 80.206 kb on + strand, within HMPREF1058_RS02835 at 80.207 kb on - strand, within HMPREF1058_RS02835 at 80.210 kb on + strand, within HMPREF1058_RS02835 at 80.210 kb on + strand, within HMPREF1058_RS02835 at 80.215 kb on - strand, within HMPREF1058_RS02835 at 80.271 kb on + strand, within HMPREF1058_RS02835 at 80.271 kb on + strand, within HMPREF1058_RS02835 at 80.418 kb on - strand, within HMPREF1058_RS02835 at 80.507 kb on - strand, within HMPREF1058_RS02835 at 80.644 kb on + strand, within HMPREF1058_RS02835 at 80.644 kb on + strand, within HMPREF1058_RS02835 at 80.649 kb on - strand, within HMPREF1058_RS02835 at 80.701 kb on - strand, within HMPREF1058_RS02835 at 80.708 kb on - strand, within HMPREF1058_RS02835 at 80.750 kb on - strand at 80.764 kb on - strand, within HMPREF1058_RS02835 at 80.764 kb on - strand, within HMPREF1058_RS02835 at 80.854 kb on + strand at 80.897 kb on - strand at 80.898 kb on + strand at 80.899 kb on - strand at 80.899 kb on - strand at 80.913 kb on + strand at 80.982 kb on - strand at 81.012 kb on - strand at 81.012 kb on - strand at 81.092 kb on - strand at 81.133 kb on + strand at 81.136 kb on + strand at 81.190 kb on - strand at 81.295 kb on + strand, within HMPREF1058_RS02830 at 81.295 kb on + strand, within HMPREF1058_RS02830 at 81.345 kb on - strand, within HMPREF1058_RS02830 at 81.487 kb on - strand, within HMPREF1058_RS02830 at 81.489 kb on - strand, within HMPREF1058_RS02830 at 81.524 kb on - strand, within HMPREF1058_RS02830 at 81.530 kb on - strand, within HMPREF1058_RS02830 at 81.549 kb on - strand, within HMPREF1058_RS02830 at 81.549 kb on - strand, within HMPREF1058_RS02830 at 81.650 kb on + strand, within HMPREF1058_RS02830 at 81.674 kb on - strand, within HMPREF1058_RS02830 at 81.682 kb on - strand, within HMPREF1058_RS02830 at 81.724 kb on + strand, within HMPREF1058_RS02830 at 81.725 kb on - strand, within HMPREF1058_RS02830 at 81.895 kb on - strand, within HMPREF1058_RS02830 at 81.912 kb on + strand, within HMPREF1058_RS02830 at 81.913 kb on - strand, within HMPREF1058_RS02830 at 81.913 kb on - strand, within HMPREF1058_RS02830 at 81.991 kb on + strand, within HMPREF1058_RS02830 at 81.992 kb on - strand, within HMPREF1058_RS02830 at 82.008 kb on - strand, within HMPREF1058_RS02830 at 82.019 kb on - strand, within HMPREF1058_RS02830 at 82.049 kb on + strand, within HMPREF1058_RS02830 at 82.069 kb on + strand, within HMPREF1058_RS02830 at 82.118 kb on + strand, within HMPREF1058_RS02830 at 82.155 kb on + strand, within HMPREF1058_RS02830 at 82.276 kb on - strand, within HMPREF1058_RS02830 at 82.403 kb on - strand, within HMPREF1058_RS02830 at 82.436 kb on + strand, within HMPREF1058_RS02830 at 82.437 kb on - strand, within HMPREF1058_RS02830 at 82.458 kb on + strand, within HMPREF1058_RS02830 at 82.480 kb on - strand, within HMPREF1058_RS02830 at 82.570 kb on + strand, within HMPREF1058_RS02830 at 82.579 kb on + strand, within HMPREF1058_RS02830 at 82.580 kb on - strand, within HMPREF1058_RS02830 at 82.652 kb on - strand, within HMPREF1058_RS02830 at 82.656 kb on + strand, within HMPREF1058_RS02830 at 82.657 kb on - strand, within HMPREF1058_RS02830 at 82.677 kb on + strand, within HMPREF1058_RS02830 at 82.696 kb on + strand, within HMPREF1058_RS02830 at 82.698 kb on + strand, within HMPREF1058_RS02830 at 82.698 kb on + strand, within HMPREF1058_RS02830 at 82.698 kb on + strand, within HMPREF1058_RS02830 at 82.698 kb on + strand, within HMPREF1058_RS02830 at 82.698 kb on + strand, within HMPREF1058_RS02830 at 82.699 kb on - strand, within HMPREF1058_RS02830 at 82.699 kb on - strand, within HMPREF1058_RS02830 at 82.699 kb on - strand, within HMPREF1058_RS02830 at 82.699 kb on - strand, within HMPREF1058_RS02830 at 82.699 kb on - strand, within HMPREF1058_RS02830 at 82.699 kb on - strand, within HMPREF1058_RS02830 at 82.699 kb on - strand, within HMPREF1058_RS02830 at 82.885 kb on - strand, within HMPREF1058_RS02830 at 82.888 kb on + strand, within HMPREF1058_RS02830 at 82.895 kb on + strand, within HMPREF1058_RS02830 at 82.979 kb on + strand, within HMPREF1058_RS02830 at 83.007 kb on + strand, within HMPREF1058_RS02830 at 83.008 kb on - strand, within HMPREF1058_RS02830 at 83.010 kb on - strand, within HMPREF1058_RS02830 at 83.048 kb on - strand, within HMPREF1058_RS02830 at 83.079 kb on - strand, within HMPREF1058_RS02830 at 83.093 kb on + strand, within HMPREF1058_RS02830 at 83.096 kb on - strand, within HMPREF1058_RS02830 at 83.096 kb on - strand, within HMPREF1058_RS02830 at 83.168 kb on - strand, within HMPREF1058_RS02830 at 83.297 kb on + strand at 83.297 kb on + strand at 83.308 kb on + strand at 83.309 kb on - strand at 83.358 kb on - strand at 83.422 kb on + strand at 83.462 kb on - strand at 83.565 kb on - strand at 83.566 kb on + strand at 83.566 kb on + strand at 83.566 kb on + strand at 83.567 kb on - strand at 83.567 kb on - strand at 83.568 kb on + strand at 83.571 kb on - strand at 83.575 kb on + strand at 83.575 kb on + strand at 83.590 kb on + strand at 83.609 kb on + strand at 83.609 kb on + strand at 83.653 kb on + strand at 83.654 kb on - strand at 83.663 kb on + strand at 83.699 kb on + strand at 83.724 kb on + strand at 83.805 kb on + strand, within HMPREF1058_RS02825 at 83.846 kb on + strand, within HMPREF1058_RS02825 at 83.848 kb on + strand, within HMPREF1058_RS02825 at 83.882 kb on + strand, within HMPREF1058_RS02825 at 83.885 kb on + strand, within HMPREF1058_RS02825 at 83.885 kb on + strand, within HMPREF1058_RS02825 at 83.886 kb on - strand, within HMPREF1058_RS02825 at 83.894 kb on + strand, within HMPREF1058_RS02825 at 83.895 kb on - strand, within HMPREF1058_RS02825 at 83.921 kb on + strand, within HMPREF1058_RS02825 at 83.923 kb on + strand, within HMPREF1058_RS02825 at 83.924 kb on - strand, within HMPREF1058_RS02825 at 84.109 kb on + strand, within HMPREF1058_RS02825 at 84.212 kb on - strand, within HMPREF1058_RS02825 at 84.325 kb on - strand, within HMPREF1058_RS02825 at 84.390 kb on + strand, within HMPREF1058_RS02825 at 84.397 kb on + strand, within HMPREF1058_RS02825 at 84.461 kb on - strand, within HMPREF1058_RS02825 at 84.468 kb on + strand, within HMPREF1058_RS02825 at 84.468 kb on + strand, within HMPREF1058_RS02825 at 84.468 kb on + strand, within HMPREF1058_RS02825 at 84.469 kb on - strand, within HMPREF1058_RS02825
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 80,060 - HMPREF1058_RS02835 0.53 +0.7 80,060 - HMPREF1058_RS02835 0.53 -0.5 80,161 + HMPREF1058_RS02835 0.57 -0.2 80,204 + HMPREF1058_RS02835 0.60 +1.7 80,206 + HMPREF1058_RS02835 0.60 +0.4 80,206 + HMPREF1058_RS02835 0.60 +0.8 80,207 - HMPREF1058_RS02835 0.60 +0.1 80,210 + HMPREF1058_RS02835 0.60 +1.0 80,210 + HMPREF1058_RS02835 0.60 +0.0 80,215 - HMPREF1058_RS02835 0.60 -2.7 80,271 + HMPREF1058_RS02835 0.63 +0.0 80,271 + HMPREF1058_RS02835 0.63 -0.5 80,418 - HMPREF1058_RS02835 0.70 -1.4 80,507 - HMPREF1058_RS02835 0.74 -0.6 80,644 + HMPREF1058_RS02835 0.81 -0.6 80,644 + HMPREF1058_RS02835 0.81 +0.2 80,649 - HMPREF1058_RS02835 0.81 +0.9 80,701 - HMPREF1058_RS02835 0.83 +0.1 80,708 - HMPREF1058_RS02835 0.84 +0.7 80,750 - +0.4 80,764 - HMPREF1058_RS02835 0.86 -0.2 80,764 - HMPREF1058_RS02835 0.86 -0.4 80,854 + +0.7 80,897 - -0.3 80,898 + +0.5 80,899 - -1.1 80,899 - -0.5 80,913 + +2.3 80,982 - -0.1 81,012 - +0.4 81,012 - -0.8 81,092 - -0.9 81,133 + -0.6 81,136 + -0.3 81,190 - -0.2 81,295 + HMPREF1058_RS02830 0.10 -0.4 81,295 + HMPREF1058_RS02830 0.10 +0.6 81,345 - HMPREF1058_RS02830 0.12 -0.9 81,487 - HMPREF1058_RS02830 0.18 -0.8 81,489 - HMPREF1058_RS02830 0.18 -0.3 81,524 - HMPREF1058_RS02830 0.20 +0.1 81,530 - HMPREF1058_RS02830 0.20 -1.2 81,549 - HMPREF1058_RS02830 0.21 -1.5 81,549 - HMPREF1058_RS02830 0.21 +0.2 81,650 + HMPREF1058_RS02830 0.25 +0.1 81,674 - HMPREF1058_RS02830 0.26 -1.5 81,682 - HMPREF1058_RS02830 0.26 +0.3 81,724 + HMPREF1058_RS02830 0.28 +0.2 81,725 - HMPREF1058_RS02830 0.28 +0.2 81,895 - HMPREF1058_RS02830 0.35 -0.8 81,912 + HMPREF1058_RS02830 0.35 +0.8 81,913 - HMPREF1058_RS02830 0.36 +0.0 81,913 - HMPREF1058_RS02830 0.36 +0.1 81,991 + HMPREF1058_RS02830 0.39 +0.7 81,992 - HMPREF1058_RS02830 0.39 +0.3 82,008 - HMPREF1058_RS02830 0.39 +0.5 82,019 - HMPREF1058_RS02830 0.40 +1.5 82,049 + HMPREF1058_RS02830 0.41 +1.4 82,069 + HMPREF1058_RS02830 0.42 -1.2 82,118 + HMPREF1058_RS02830 0.44 +1.0 82,155 + HMPREF1058_RS02830 0.45 +0.8 82,276 - HMPREF1058_RS02830 0.50 -1.0 82,403 - HMPREF1058_RS02830 0.56 -1.3 82,436 + HMPREF1058_RS02830 0.57 +0.1 82,437 - HMPREF1058_RS02830 0.57 -0.3 82,458 + HMPREF1058_RS02830 0.58 +1.0 82,480 - HMPREF1058_RS02830 0.59 -0.5 82,570 + HMPREF1058_RS02830 0.62 -0.1 82,579 + HMPREF1058_RS02830 0.63 -0.5 82,580 - HMPREF1058_RS02830 0.63 -1.8 82,652 - HMPREF1058_RS02830 0.66 -0.6 82,656 + HMPREF1058_RS02830 0.66 -1.1 82,657 - HMPREF1058_RS02830 0.66 +0.8 82,677 + HMPREF1058_RS02830 0.67 -0.8 82,696 + HMPREF1058_RS02830 0.68 -0.1 82,698 + HMPREF1058_RS02830 0.68 -0.2 82,698 + HMPREF1058_RS02830 0.68 +1.3 82,698 + HMPREF1058_RS02830 0.68 -0.4 82,698 + HMPREF1058_RS02830 0.68 -0.5 82,698 + HMPREF1058_RS02830 0.68 +0.3 82,699 - HMPREF1058_RS02830 0.68 -1.0 82,699 - HMPREF1058_RS02830 0.68 -0.9 82,699 - HMPREF1058_RS02830 0.68 -0.2 82,699 - HMPREF1058_RS02830 0.68 -0.1 82,699 - HMPREF1058_RS02830 0.68 +0.1 82,699 - HMPREF1058_RS02830 0.68 -0.0 82,699 - HMPREF1058_RS02830 0.68 -0.3 82,885 - HMPREF1058_RS02830 0.75 -1.6 82,888 + HMPREF1058_RS02830 0.75 -0.1 82,895 + HMPREF1058_RS02830 0.76 +1.1 82,979 + HMPREF1058_RS02830 0.79 +0.4 83,007 + HMPREF1058_RS02830 0.80 -0.2 83,008 - HMPREF1058_RS02830 0.80 -0.8 83,010 - HMPREF1058_RS02830 0.80 -0.2 83,048 - HMPREF1058_RS02830 0.82 -0.7 83,079 - HMPREF1058_RS02830 0.83 -0.5 83,093 + HMPREF1058_RS02830 0.84 +0.3 83,096 - HMPREF1058_RS02830 0.84 +0.8 83,096 - HMPREF1058_RS02830 0.84 -0.2 83,168 - HMPREF1058_RS02830 0.87 +2.1 83,297 + -0.7 83,297 + +1.3 83,308 + +0.1 83,309 - -1.6 83,358 - +1.3 83,422 + +0.3 83,462 - -0.6 83,565 - +0.5 83,566 + -2.9 83,566 + -0.2 83,566 + +0.0 83,567 - -0.6 83,567 - -0.0 83,568 + -0.5 83,571 - -0.8 83,575 + -0.6 83,575 + -0.1 83,590 + -0.4 83,609 + +0.3 83,609 + +0.7 83,653 + +0.1 83,654 - +0.2 83,663 + -0.6 83,699 + -0.0 83,724 + +1.3 83,805 + HMPREF1058_RS02825 0.14 -1.4 83,846 + HMPREF1058_RS02825 0.17 +3.6 83,848 + HMPREF1058_RS02825 0.17 -0.7 83,882 + HMPREF1058_RS02825 0.19 -0.5 83,885 + HMPREF1058_RS02825 0.19 -0.1 83,885 + HMPREF1058_RS02825 0.19 -2.0 83,886 - HMPREF1058_RS02825 0.19 -0.3 83,894 + HMPREF1058_RS02825 0.20 -1.5 83,895 - HMPREF1058_RS02825 0.20 -0.6 83,921 + HMPREF1058_RS02825 0.22 +0.2 83,923 + HMPREF1058_RS02825 0.22 -1.8 83,924 - HMPREF1058_RS02825 0.22 -1.2 84,109 + HMPREF1058_RS02825 0.34 +0.5 84,212 - HMPREF1058_RS02825 0.40 -1.3 84,325 - HMPREF1058_RS02825 0.48 -1.9 84,390 + HMPREF1058_RS02825 0.52 +0.1 84,397 + HMPREF1058_RS02825 0.52 +0.6 84,461 - HMPREF1058_RS02825 0.57 -0.8 84,468 + HMPREF1058_RS02825 0.57 +0.1 84,468 + HMPREF1058_RS02825 0.57 -1.0 84,468 + HMPREF1058_RS02825 0.57 +1.3 84,469 - HMPREF1058_RS02825 0.57 +0.2
Or see this region's nucleotide sequence