Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS02020 and HMPREF1058_RS02015 overlap by 4 nucleotides HMPREF1058_RS02015 and HMPREF1058_RS02010 are separated by 207 nucleotides HMPREF1058_RS02010 and HMPREF1058_RS02005 are separated by 90 nucleotides
HMPREF1058_RS02020: HMPREF1058_RS02020 - hypothetical protein, at 546 to 923
_RS02020
HMPREF1058_RS02015: HMPREF1058_RS02015 - ASCH domain-containing protein, at 920 to 1,465
_RS02015
HMPREF1058_RS02010: HMPREF1058_RS02010 - hypothetical protein, at 1,673 to 1,855
_RS02010
HMPREF1058_RS02005: HMPREF1058_RS02005 - hypothetical protein, at 1,946 to 2,383
_RS02005
Position (kb) 0
1
2 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 0.098 kb on + strand at 0.099 kb on - strand at 0.129 kb on - strand at 0.129 kb on - strand at 0.137 kb on + strand at 0.151 kb on - strand at 0.167 kb on + strand at 0.196 kb on - strand at 0.240 kb on - strand at 0.324 kb on - strand at 0.378 kb on + strand at 0.433 kb on + strand at 0.434 kb on - strand at 0.435 kb on + strand at 0.453 kb on + strand at 0.454 kb on - strand at 0.456 kb on - strand at 0.456 kb on - strand at 0.495 kb on + strand at 0.512 kb on + strand at 0.513 kb on - strand at 0.513 kb on - strand at 0.514 kb on + strand at 0.523 kb on - strand at 0.528 kb on - strand at 0.574 kb on - strand at 0.585 kb on - strand, within HMPREF1058_RS02020 at 0.663 kb on - strand, within HMPREF1058_RS02020 at 0.787 kb on - strand, within HMPREF1058_RS02020 at 0.787 kb on - strand, within HMPREF1058_RS02020 at 0.829 kb on + strand, within HMPREF1058_RS02020 at 0.830 kb on - strand, within HMPREF1058_RS02020 at 0.896 kb on + strand at 0.897 kb on - strand at 0.898 kb on + strand at 0.902 kb on - strand at 0.936 kb on + strand at 0.991 kb on + strand, within HMPREF1058_RS02015 at 1.014 kb on - strand, within HMPREF1058_RS02015 at 1.029 kb on + strand, within HMPREF1058_RS02015 at 1.061 kb on + strand, within HMPREF1058_RS02015 at 1.062 kb on - strand, within HMPREF1058_RS02015 at 1.074 kb on - strand, within HMPREF1058_RS02015 at 1.087 kb on + strand, within HMPREF1058_RS02015 at 1.291 kb on - strand, within HMPREF1058_RS02015 at 1.329 kb on + strand, within HMPREF1058_RS02015 at 1.330 kb on - strand, within HMPREF1058_RS02015 at 1.353 kb on + strand, within HMPREF1058_RS02015 at 1.359 kb on + strand at 1.360 kb on - strand, within HMPREF1058_RS02015 at 1.375 kb on + strand, within HMPREF1058_RS02015 at 1.376 kb on - strand, within HMPREF1058_RS02015 at 1.405 kb on + strand, within HMPREF1058_RS02015 at 1.406 kb on - strand, within HMPREF1058_RS02015 at 1.411 kb on + strand at 1.412 kb on - strand at 1.415 kb on - strand at 1.507 kb on - strand at 1.539 kb on + strand at 1.540 kb on - strand at 1.650 kb on + strand at 1.650 kb on + strand at 1.661 kb on - strand at 1.666 kb on + strand at 1.712 kb on - strand, within HMPREF1058_RS02010 at 1.747 kb on - strand, within HMPREF1058_RS02010 at 1.771 kb on - strand, within HMPREF1058_RS02010 at 1.796 kb on + strand, within HMPREF1058_RS02010 at 1.797 kb on - strand, within HMPREF1058_RS02010 at 1.798 kb on + strand, within HMPREF1058_RS02010 at 1.798 kb on + strand, within HMPREF1058_RS02010 at 1.799 kb on - strand, within HMPREF1058_RS02010 at 1.809 kb on - strand, within HMPREF1058_RS02010 at 1.917 kb on - strand at 1.961 kb on - strand at 2.072 kb on + strand, within HMPREF1058_RS02005 at 2.119 kb on + strand, within HMPREF1058_RS02005 at 2.119 kb on + strand, within HMPREF1058_RS02005 at 2.258 kb on - strand, within HMPREF1058_RS02005 at 2.325 kb on + strand, within HMPREF1058_RS02005 at 2.325 kb on + strand, within HMPREF1058_RS02005 at 2.330 kb on + strand, within HMPREF1058_RS02005 at 2.331 kb on - strand, within HMPREF1058_RS02005 at 2.331 kb on - strand, within HMPREF1058_RS02005 at 2.421 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 98 + +1.8 99 - +0.5 129 - +0.1 129 - -1.0 137 + +2.3 151 - +0.6 167 + +3.9 196 - +0.2 240 - -0.5 324 - -0.4 378 + +3.7 433 + +2.9 434 - -0.2 435 + +4.0 453 + +3.3 454 - -0.5 456 - -0.0 456 - -0.6 495 + +3.2 512 + +2.1 513 - -1.8 513 - +0.1 514 + +2.2 523 - +0.1 528 - +1.0 574 - +1.2 585 - HMPREF1058_RS02020 0.10 -1.9 663 - HMPREF1058_RS02020 0.31 +0.4 787 - HMPREF1058_RS02020 0.64 +0.8 787 - HMPREF1058_RS02020 0.64 -1.0 829 + HMPREF1058_RS02020 0.75 +2.9 830 - HMPREF1058_RS02020 0.75 -1.2 896 + +2.5 897 - +1.5 898 + +2.3 902 - -1.2 936 + +1.9 991 + HMPREF1058_RS02015 0.13 +1.0 1,014 - HMPREF1058_RS02015 0.17 +0.3 1,029 + HMPREF1058_RS02015 0.20 +1.5 1,061 + HMPREF1058_RS02015 0.26 +0.5 1,062 - HMPREF1058_RS02015 0.26 -3.0 1,074 - HMPREF1058_RS02015 0.28 +0.1 1,087 + HMPREF1058_RS02015 0.31 +0.9 1,291 - HMPREF1058_RS02015 0.68 +0.2 1,329 + HMPREF1058_RS02015 0.75 +1.9 1,330 - HMPREF1058_RS02015 0.75 +0.9 1,353 + HMPREF1058_RS02015 0.79 +0.6 1,359 + +0.1 1,360 - HMPREF1058_RS02015 0.81 -1.2 1,375 + HMPREF1058_RS02015 0.83 +0.9 1,376 - HMPREF1058_RS02015 0.84 +0.9 1,405 + HMPREF1058_RS02015 0.89 +1.9 1,406 - HMPREF1058_RS02015 0.89 +0.3 1,411 + +3.1 1,412 - -1.0 1,415 - -0.5 1,507 - +1.3 1,539 + +1.7 1,540 - -0.4 1,650 + +3.8 1,650 + +3.3 1,661 - +0.1 1,666 + +0.1 1,712 - HMPREF1058_RS02010 0.21 +2.5 1,747 - HMPREF1058_RS02010 0.40 -1.0 1,771 - HMPREF1058_RS02010 0.54 -1.3 1,796 + HMPREF1058_RS02010 0.67 +0.3 1,797 - HMPREF1058_RS02010 0.68 +0.6 1,798 + HMPREF1058_RS02010 0.68 -0.9 1,798 + HMPREF1058_RS02010 0.68 -1.1 1,799 - HMPREF1058_RS02010 0.69 -0.1 1,809 - HMPREF1058_RS02010 0.74 -0.4 1,917 - -0.0 1,961 - +1.7 2,072 + HMPREF1058_RS02005 0.29 -0.9 2,119 + HMPREF1058_RS02005 0.39 +1.2 2,119 + HMPREF1058_RS02005 0.39 -1.1 2,258 - HMPREF1058_RS02005 0.71 +2.2 2,325 + HMPREF1058_RS02005 0.87 -0.5 2,325 + HMPREF1058_RS02005 0.87 -0.7 2,330 + HMPREF1058_RS02005 0.88 +0.8 2,331 - HMPREF1058_RS02005 0.88 +0.6 2,331 - HMPREF1058_RS02005 0.88 +0.1 2,421 - -0.1
Or see this region's nucleotide sequence