Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS01250 and HMPREF1058_RS01245 are separated by 8 nucleotides HMPREF1058_RS01245 and HMPREF1058_RS01240 are separated by 385 nucleotides HMPREF1058_RS01240 and HMPREF1058_RS01235 are separated by 20 nucleotides
HMPREF1058_RS01250: HMPREF1058_RS01250 - DUF4738 domain-containing protein, at 170,776 to 171,564
_RS01250
HMPREF1058_RS01245: HMPREF1058_RS01245 - hypothetical protein, at 171,573 to 171,890
_RS01245
HMPREF1058_RS01240: HMPREF1058_RS01240 - DEAD/DEAH box helicase, at 172,276 to 173,553
_RS01240
HMPREF1058_RS01235: HMPREF1058_RS01235 - phosphoserine phosphatase SerB, at 173,574 to 174,806
_RS01235
Position (kb) 172
173
174 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 171.299 kb on + strand, within HMPREF1058_RS01250 at 171.300 kb on - strand, within HMPREF1058_RS01250 at 171.302 kb on - strand, within HMPREF1058_RS01250 at 171.302 kb on - strand, within HMPREF1058_RS01250 at 171.304 kb on + strand, within HMPREF1058_RS01250 at 171.305 kb on - strand, within HMPREF1058_RS01250 at 171.312 kb on + strand, within HMPREF1058_RS01250 at 171.313 kb on - strand, within HMPREF1058_RS01250 at 171.317 kb on + strand, within HMPREF1058_RS01250 at 171.318 kb on - strand, within HMPREF1058_RS01250 at 171.332 kb on - strand, within HMPREF1058_RS01250 at 171.332 kb on - strand, within HMPREF1058_RS01250 at 171.353 kb on - strand, within HMPREF1058_RS01250 at 171.419 kb on + strand, within HMPREF1058_RS01250 at 171.505 kb on + strand at 171.505 kb on + strand at 171.508 kb on - strand at 171.573 kb on - strand at 171.600 kb on + strand at 171.601 kb on - strand at 172.034 kb on + strand at 172.035 kb on - strand at 172.039 kb on + strand at 172.040 kb on - strand at 172.040 kb on - strand at 172.080 kb on + strand at 172.081 kb on - strand at 172.095 kb on - strand at 172.103 kb on + strand at 172.123 kb on + strand at 172.123 kb on + strand at 172.125 kb on + strand at 172.125 kb on + strand at 172.125 kb on + strand at 172.126 kb on - strand at 172.126 kb on - strand at 172.126 kb on - strand at 172.126 kb on - strand at 172.126 kb on - strand at 172.127 kb on + strand at 172.145 kb on + strand at 172.145 kb on + strand at 172.145 kb on + strand at 172.145 kb on + strand at 172.145 kb on + strand at 172.146 kb on - strand at 172.343 kb on + strand at 172.343 kb on + strand at 172.343 kb on + strand at 172.343 kb on + strand at 172.452 kb on - strand, within HMPREF1058_RS01240 at 172.519 kb on - strand, within HMPREF1058_RS01240 at 172.552 kb on - strand, within HMPREF1058_RS01240 at 172.571 kb on + strand, within HMPREF1058_RS01240 at 172.572 kb on - strand, within HMPREF1058_RS01240 at 172.572 kb on - strand, within HMPREF1058_RS01240 at 172.599 kb on - strand, within HMPREF1058_RS01240 at 172.630 kb on + strand, within HMPREF1058_RS01240 at 172.631 kb on - strand, within HMPREF1058_RS01240 at 172.695 kb on + strand, within HMPREF1058_RS01240 at 172.695 kb on + strand, within HMPREF1058_RS01240 at 172.741 kb on + strand, within HMPREF1058_RS01240 at 172.742 kb on - strand, within HMPREF1058_RS01240 at 172.851 kb on + strand, within HMPREF1058_RS01240 at 172.852 kb on - strand, within HMPREF1058_RS01240 at 172.881 kb on + strand, within HMPREF1058_RS01240 at 172.881 kb on + strand, within HMPREF1058_RS01240 at 172.881 kb on + strand, within HMPREF1058_RS01240 at 172.882 kb on - strand at 172.882 kb on - strand, within HMPREF1058_RS01240 at 172.883 kb on + strand, within HMPREF1058_RS01240 at 172.883 kb on + strand, within HMPREF1058_RS01240 at 172.884 kb on - strand, within HMPREF1058_RS01240 at 172.972 kb on + strand, within HMPREF1058_RS01240 at 173.028 kb on - strand, within HMPREF1058_RS01240 at 173.043 kb on + strand, within HMPREF1058_RS01240 at 173.074 kb on - strand, within HMPREF1058_RS01240 at 173.120 kb on - strand, within HMPREF1058_RS01240 at 173.123 kb on + strand, within HMPREF1058_RS01240 at 173.160 kb on + strand, within HMPREF1058_RS01240 at 173.170 kb on + strand, within HMPREF1058_RS01240 at 173.290 kb on + strand, within HMPREF1058_RS01240 at 173.464 kb on - strand at 173.474 kb on - strand at 173.555 kb on + strand at 173.557 kb on + strand at 173.558 kb on - strand at 173.558 kb on - strand at 173.558 kb on - strand at 173.558 kb on - strand at 173.558 kb on - strand at 173.559 kb on + strand at 173.559 kb on + strand at 173.560 kb on - strand at 173.599 kb on + strand at 173.649 kb on - strand at 173.649 kb on - strand at 173.676 kb on + strand at 173.752 kb on - strand, within HMPREF1058_RS01235 at 173.760 kb on - strand, within HMPREF1058_RS01235 at 173.760 kb on - strand, within HMPREF1058_RS01235 at 173.769 kb on + strand, within HMPREF1058_RS01235 at 173.769 kb on + strand, within HMPREF1058_RS01235 at 173.770 kb on - strand, within HMPREF1058_RS01235 at 173.816 kb on + strand, within HMPREF1058_RS01235 at 173.833 kb on - strand, within HMPREF1058_RS01235 at 173.833 kb on - strand, within HMPREF1058_RS01235 at 173.857 kb on - strand, within HMPREF1058_RS01235 at 173.893 kb on - strand, within HMPREF1058_RS01235 at 173.893 kb on - strand, within HMPREF1058_RS01235 at 173.897 kb on - strand, within HMPREF1058_RS01235 at 173.912 kb on + strand, within HMPREF1058_RS01235 at 173.913 kb on - strand, within HMPREF1058_RS01235 at 173.923 kb on + strand, within HMPREF1058_RS01235 at 173.924 kb on - strand, within HMPREF1058_RS01235 at 173.935 kb on + strand, within HMPREF1058_RS01235 at 173.936 kb on - strand, within HMPREF1058_RS01235 at 174.183 kb on - strand, within HMPREF1058_RS01235 at 174.196 kb on - strand, within HMPREF1058_RS01235 at 174.248 kb on + strand, within HMPREF1058_RS01235 at 174.248 kb on + strand, within HMPREF1058_RS01235 at 174.248 kb on + strand, within HMPREF1058_RS01235 at 174.357 kb on - strand, within HMPREF1058_RS01235 at 174.366 kb on + strand, within HMPREF1058_RS01235 at 174.405 kb on - strand, within HMPREF1058_RS01235 at 174.447 kb on + strand, within HMPREF1058_RS01235 at 174.447 kb on + strand at 174.448 kb on - strand, within HMPREF1058_RS01235
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 171,299 + HMPREF1058_RS01250 0.66 +0.7 171,300 - HMPREF1058_RS01250 0.66 +0.8 171,302 - HMPREF1058_RS01250 0.67 -0.1 171,302 - HMPREF1058_RS01250 0.67 +0.5 171,304 + HMPREF1058_RS01250 0.67 -0.0 171,305 - HMPREF1058_RS01250 0.67 +1.4 171,312 + HMPREF1058_RS01250 0.68 -0.7 171,313 - HMPREF1058_RS01250 0.68 -0.1 171,317 + HMPREF1058_RS01250 0.69 -0.7 171,318 - HMPREF1058_RS01250 0.69 +0.0 171,332 - HMPREF1058_RS01250 0.70 +1.3 171,332 - HMPREF1058_RS01250 0.70 -2.0 171,353 - HMPREF1058_RS01250 0.73 -0.9 171,419 + HMPREF1058_RS01250 0.81 +0.0 171,505 + +0.7 171,505 + +0.2 171,508 - +0.8 171,573 - -0.3 171,600 + +0.7 171,601 - -1.2 172,034 + +0.9 172,035 - +0.4 172,039 + +0.1 172,040 - -0.4 172,040 - +0.4 172,080 + +2.6 172,081 - -0.1 172,095 - +0.5 172,103 + -1.4 172,123 + +0.1 172,123 + +0.4 172,125 + +0.2 172,125 + +0.7 172,125 + +0.1 172,126 - +1.0 172,126 - +0.3 172,126 - +0.5 172,126 - -0.8 172,126 - +0.1 172,127 + +0.1 172,145 + -0.2 172,145 + +0.9 172,145 + -1.7 172,145 + +0.8 172,145 + -0.3 172,146 - +1.5 172,343 + +1.5 172,343 + +1.6 172,343 + +0.4 172,343 + +0.4 172,452 - HMPREF1058_RS01240 0.14 +0.8 172,519 - HMPREF1058_RS01240 0.19 +1.4 172,552 - HMPREF1058_RS01240 0.22 +0.8 172,571 + HMPREF1058_RS01240 0.23 +0.2 172,572 - HMPREF1058_RS01240 0.23 -0.3 172,572 - HMPREF1058_RS01240 0.23 +1.0 172,599 - HMPREF1058_RS01240 0.25 +0.6 172,630 + HMPREF1058_RS01240 0.28 +2.1 172,631 - HMPREF1058_RS01240 0.28 +1.0 172,695 + HMPREF1058_RS01240 0.33 +1.2 172,695 + HMPREF1058_RS01240 0.33 +1.3 172,741 + HMPREF1058_RS01240 0.36 +0.7 172,742 - HMPREF1058_RS01240 0.36 -0.9 172,851 + HMPREF1058_RS01240 0.45 +0.8 172,852 - HMPREF1058_RS01240 0.45 +1.5 172,881 + HMPREF1058_RS01240 0.47 -0.2 172,881 + HMPREF1058_RS01240 0.47 +0.7 172,881 + HMPREF1058_RS01240 0.47 +0.5 172,882 - +1.5 172,882 - HMPREF1058_RS01240 0.47 +1.2 172,883 + HMPREF1058_RS01240 0.47 +0.9 172,883 + HMPREF1058_RS01240 0.47 +1.0 172,884 - HMPREF1058_RS01240 0.48 +0.8 172,972 + HMPREF1058_RS01240 0.54 -0.3 173,028 - HMPREF1058_RS01240 0.59 +0.1 173,043 + HMPREF1058_RS01240 0.60 +0.6 173,074 - HMPREF1058_RS01240 0.62 +1.2 173,120 - HMPREF1058_RS01240 0.66 +0.1 173,123 + HMPREF1058_RS01240 0.66 +0.5 173,160 + HMPREF1058_RS01240 0.69 +0.1 173,170 + HMPREF1058_RS01240 0.70 +0.7 173,290 + HMPREF1058_RS01240 0.79 +1.3 173,464 - +0.0 173,474 - +1.2 173,555 + +1.5 173,557 + +0.1 173,558 - -0.9 173,558 - -0.0 173,558 - +0.7 173,558 - +0.8 173,558 - +0.5 173,559 + +1.4 173,559 + -0.1 173,560 - +0.7 173,599 + -0.1 173,649 - +0.2 173,649 - -0.2 173,676 + +1.0 173,752 - HMPREF1058_RS01235 0.14 -0.6 173,760 - HMPREF1058_RS01235 0.15 +0.0 173,760 - HMPREF1058_RS01235 0.15 +0.4 173,769 + HMPREF1058_RS01235 0.16 +1.7 173,769 + HMPREF1058_RS01235 0.16 +0.2 173,770 - HMPREF1058_RS01235 0.16 -0.4 173,816 + HMPREF1058_RS01235 0.20 +1.0 173,833 - HMPREF1058_RS01235 0.21 -0.5 173,833 - HMPREF1058_RS01235 0.21 +0.1 173,857 - HMPREF1058_RS01235 0.23 -0.6 173,893 - HMPREF1058_RS01235 0.26 -0.1 173,893 - HMPREF1058_RS01235 0.26 +2.9 173,897 - HMPREF1058_RS01235 0.26 +0.3 173,912 + HMPREF1058_RS01235 0.27 -1.0 173,913 - HMPREF1058_RS01235 0.27 +0.3 173,923 + HMPREF1058_RS01235 0.28 +2.5 173,924 - HMPREF1058_RS01235 0.28 +0.5 173,935 + HMPREF1058_RS01235 0.29 +1.1 173,936 - HMPREF1058_RS01235 0.29 +1.5 174,183 - HMPREF1058_RS01235 0.49 -0.9 174,196 - HMPREF1058_RS01235 0.50 -1.1 174,248 + HMPREF1058_RS01235 0.55 -0.2 174,248 + HMPREF1058_RS01235 0.55 +0.1 174,248 + HMPREF1058_RS01235 0.55 +4.3 174,357 - HMPREF1058_RS01235 0.64 -1.2 174,366 + HMPREF1058_RS01235 0.64 -0.8 174,405 - HMPREF1058_RS01235 0.67 +0.7 174,447 + HMPREF1058_RS01235 0.71 -0.0 174,447 + +0.1 174,448 - HMPREF1058_RS01235 0.71 -0.0
Or see this region's nucleotide sequence