Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS00480 and HMPREF1058_RS00475 overlap by 4 nucleotides HMPREF1058_RS00475 and HMPREF1058_RS00470 are separated by 4 nucleotides HMPREF1058_RS00470 and HMPREF1058_RS00465 are separated by 117 nucleotides HMPREF1058_RS00465 and HMPREF1058_RS00460 are separated by 59 nucleotides
HMPREF1058_RS00480: HMPREF1058_RS00480 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 66,744 to 67,226
_RS00480
HMPREF1058_RS00475: HMPREF1058_RS00475 - fumarylacetoacetate hydrolase family protein, at 67,223 to 67,840
_RS00475
HMPREF1058_RS00470: HMPREF1058_RS00470 - redox-sensing transcriptional repressor Rex, at 67,845 to 68,495
_RS00470
HMPREF1058_RS00465: HMPREF1058_RS00465 - translation initiation factor, at 68,613 to 68,951
_RS00465
HMPREF1058_RS00460: HMPREF1058_RS00460 - elongation factor Ts, at 69,011 to 70,000
_RS00460
Position (kb) 67
68
69 Strain fitness (log2 ratio) -2
-1
0
1
2 at 67.319 kb on - strand, within HMPREF1058_RS00475 at 67.431 kb on - strand, within HMPREF1058_RS00475 at 67.431 kb on - strand, within HMPREF1058_RS00475 at 67.431 kb on - strand, within HMPREF1058_RS00475 at 67.494 kb on - strand, within HMPREF1058_RS00475 at 67.508 kb on - strand, within HMPREF1058_RS00475 at 67.584 kb on - strand, within HMPREF1058_RS00475 at 67.606 kb on - strand, within HMPREF1058_RS00475 at 67.655 kb on - strand, within HMPREF1058_RS00475 at 67.655 kb on - strand, within HMPREF1058_RS00475 at 67.844 kb on - strand at 67.872 kb on - strand at 67.989 kb on - strand, within HMPREF1058_RS00470 at 67.989 kb on - strand, within HMPREF1058_RS00470 at 67.996 kb on - strand, within HMPREF1058_RS00470 at 68.008 kb on - strand, within HMPREF1058_RS00470 at 68.013 kb on - strand, within HMPREF1058_RS00470 at 68.013 kb on - strand, within HMPREF1058_RS00470 at 68.016 kb on - strand, within HMPREF1058_RS00470 at 68.127 kb on - strand, within HMPREF1058_RS00470 at 68.314 kb on - strand, within HMPREF1058_RS00470 at 68.314 kb on - strand, within HMPREF1058_RS00470 at 68.314 kb on - strand, within HMPREF1058_RS00470 at 68.368 kb on - strand, within HMPREF1058_RS00470 at 68.484 kb on - strand at 68.484 kb on - strand at 68.484 kb on - strand at 68.488 kb on - strand at 68.510 kb on - strand at 68.652 kb on + strand, within HMPREF1058_RS00465 at 68.714 kb on + strand, within HMPREF1058_RS00465 at 68.863 kb on + strand, within HMPREF1058_RS00465 at 68.936 kb on + strand at 68.995 kb on - strand at 68.995 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 67,319 - HMPREF1058_RS00475 0.16 -0.1 67,431 - HMPREF1058_RS00475 0.34 -2.4 67,431 - HMPREF1058_RS00475 0.34 +0.3 67,431 - HMPREF1058_RS00475 0.34 -0.0 67,494 - HMPREF1058_RS00475 0.44 +0.2 67,508 - HMPREF1058_RS00475 0.46 -0.4 67,584 - HMPREF1058_RS00475 0.58 +0.1 67,606 - HMPREF1058_RS00475 0.62 +1.0 67,655 - HMPREF1058_RS00475 0.70 -0.2 67,655 - HMPREF1058_RS00475 0.70 +0.2 67,844 - -0.6 67,872 - +0.2 67,989 - HMPREF1058_RS00470 0.22 -0.6 67,989 - HMPREF1058_RS00470 0.22 +1.7 67,996 - HMPREF1058_RS00470 0.23 -1.0 68,008 - HMPREF1058_RS00470 0.25 +0.3 68,013 - HMPREF1058_RS00470 0.26 +1.1 68,013 - HMPREF1058_RS00470 0.26 +0.6 68,016 - HMPREF1058_RS00470 0.26 +0.6 68,127 - HMPREF1058_RS00470 0.43 +0.4 68,314 - HMPREF1058_RS00470 0.72 +0.2 68,314 - HMPREF1058_RS00470 0.72 +0.0 68,314 - HMPREF1058_RS00470 0.72 +0.4 68,368 - HMPREF1058_RS00470 0.80 +0.6 68,484 - +1.1 68,484 - -0.6 68,484 - +0.6 68,488 - +0.6 68,510 - -0.3 68,652 + HMPREF1058_RS00465 0.12 +0.0 68,714 + HMPREF1058_RS00465 0.30 +2.1 68,863 + HMPREF1058_RS00465 0.74 +0.2 68,936 + -1.0 68,995 - -0.3 68,995 - +0.3
Or see this region's nucleotide sequence