Experiment: Sucrose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS08305 and HMPREF1058_RS08300 overlap by 4 nucleotides HMPREF1058_RS08300 and HMPREF1058_RS08295 are separated by 1 nucleotides HMPREF1058_RS08295 and HMPREF1058_RS08290 are separated by 220 nucleotides HMPREF1058_RS08290 and HMPREF1058_RS08285 are separated by 22 nucleotides
HMPREF1058_RS08305: HMPREF1058_RS08305 - DJ-1/PfpI family protein, at 1,195,349 to 1,195,900
_RS08305
HMPREF1058_RS08300: HMPREF1058_RS08300 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,195,897 to 1,196,556
_RS08300
HMPREF1058_RS08295: HMPREF1058_RS08295 - ATP-dependent DNA helicase RecG, at 1,196,558 to 1,198,654
_RS08295
HMPREF1058_RS08290: HMPREF1058_RS08290 - nucleoside-diphosphate kinase, at 1,198,875 to 1,199,339
_RS08290
HMPREF1058_RS08285: HMPREF1058_RS08285 - peptidoglycan DD-metalloendopeptidase family protein, at 1,199,362 to 1,200,252
_RS08285
Position (kb)
1196
1197
1198
1199 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1195.673 kb on + strand, within HMPREF1058_RS08305 at 1195.707 kb on + strand, within HMPREF1058_RS08305 at 1195.707 kb on + strand, within HMPREF1058_RS08305 at 1195.751 kb on + strand, within HMPREF1058_RS08305 at 1196.573 kb on - strand at 1196.680 kb on + strand at 1196.681 kb on - strand at 1196.708 kb on + strand at 1196.708 kb on + strand at 1196.738 kb on + strand at 1196.738 kb on + strand at 1196.738 kb on + strand at 1196.738 kb on + strand at 1196.739 kb on - strand at 1196.739 kb on - strand at 1196.739 kb on - strand at 1196.739 kb on - strand at 1196.880 kb on - strand, within HMPREF1058_RS08295 at 1196.881 kb on + strand, within HMPREF1058_RS08295 at 1196.897 kb on + strand, within HMPREF1058_RS08295 at 1196.898 kb on - strand, within HMPREF1058_RS08295 at 1197.145 kb on - strand, within HMPREF1058_RS08295 at 1197.145 kb on - strand, within HMPREF1058_RS08295 at 1197.242 kb on + strand, within HMPREF1058_RS08295 at 1197.257 kb on - strand, within HMPREF1058_RS08295 at 1197.329 kb on - strand, within HMPREF1058_RS08295 at 1197.520 kb on + strand, within HMPREF1058_RS08295 at 1197.520 kb on + strand, within HMPREF1058_RS08295 at 1197.521 kb on - strand, within HMPREF1058_RS08295 at 1197.521 kb on - strand, within HMPREF1058_RS08295 at 1197.521 kb on - strand, within HMPREF1058_RS08295 at 1197.646 kb on - strand, within HMPREF1058_RS08295 at 1197.682 kb on + strand, within HMPREF1058_RS08295 at 1197.682 kb on + strand, within HMPREF1058_RS08295 at 1197.866 kb on + strand, within HMPREF1058_RS08295 at 1197.866 kb on + strand, within HMPREF1058_RS08295 at 1197.866 kb on + strand, within HMPREF1058_RS08295 at 1197.882 kb on + strand, within HMPREF1058_RS08295 at 1197.962 kb on + strand, within HMPREF1058_RS08295 at 1198.067 kb on + strand, within HMPREF1058_RS08295 at 1198.195 kb on + strand, within HMPREF1058_RS08295 at 1198.323 kb on - strand, within HMPREF1058_RS08295 at 1198.380 kb on + strand, within HMPREF1058_RS08295 at 1198.381 kb on - strand, within HMPREF1058_RS08295 at 1198.550 kb on - strand at 1198.555 kb on - strand at 1198.633 kb on - strand at 1198.764 kb on - strand at 1198.764 kb on - strand at 1198.871 kb on + strand at 1198.940 kb on + strand, within HMPREF1058_RS08290 at 1198.940 kb on + strand, within HMPREF1058_RS08290 at 1199.022 kb on + strand, within HMPREF1058_RS08290 at 1199.213 kb on + strand, within HMPREF1058_RS08290 at 1199.215 kb on + strand, within HMPREF1058_RS08290 at 1199.215 kb on + strand, within HMPREF1058_RS08290 at 1199.294 kb on + strand at 1199.324 kb on + strand at 1199.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose 10 mM (C) remove 1,195,673 + HMPREF1058_RS08305 0.59 -2.0 1,195,707 + HMPREF1058_RS08305 0.65 +1.1 1,195,707 + HMPREF1058_RS08305 0.65 -0.7 1,195,751 + HMPREF1058_RS08305 0.73 +1.9 1,196,573 - -1.8 1,196,680 + -1.8 1,196,681 - -0.4 1,196,708 + -2.3 1,196,708 + -0.1 1,196,738 + -1.2 1,196,738 + -2.1 1,196,738 + -1.3 1,196,738 + -0.1 1,196,739 - -2.0 1,196,739 - -1.3 1,196,739 - -1.7 1,196,739 - -2.4 1,196,880 - HMPREF1058_RS08295 0.15 -0.8 1,196,881 + HMPREF1058_RS08295 0.15 -1.7 1,196,897 + HMPREF1058_RS08295 0.16 -2.7 1,196,898 - HMPREF1058_RS08295 0.16 +0.1 1,197,145 - HMPREF1058_RS08295 0.28 -1.4 1,197,145 - HMPREF1058_RS08295 0.28 -1.7 1,197,242 + HMPREF1058_RS08295 0.33 -2.3 1,197,257 - HMPREF1058_RS08295 0.33 -1.9 1,197,329 - HMPREF1058_RS08295 0.37 -0.5 1,197,520 + HMPREF1058_RS08295 0.46 -2.9 1,197,520 + HMPREF1058_RS08295 0.46 -1.9 1,197,521 - HMPREF1058_RS08295 0.46 +0.3 1,197,521 - HMPREF1058_RS08295 0.46 -0.7 1,197,521 - HMPREF1058_RS08295 0.46 -2.4 1,197,646 - HMPREF1058_RS08295 0.52 -1.7 1,197,682 + HMPREF1058_RS08295 0.54 -1.8 1,197,682 + HMPREF1058_RS08295 0.54 -3.6 1,197,866 + HMPREF1058_RS08295 0.62 +0.1 1,197,866 + HMPREF1058_RS08295 0.62 -3.5 1,197,866 + HMPREF1058_RS08295 0.62 -0.9 1,197,882 + HMPREF1058_RS08295 0.63 -1.4 1,197,962 + HMPREF1058_RS08295 0.67 -2.1 1,198,067 + HMPREF1058_RS08295 0.72 -1.0 1,198,195 + HMPREF1058_RS08295 0.78 -2.5 1,198,323 - HMPREF1058_RS08295 0.84 -0.4 1,198,380 + HMPREF1058_RS08295 0.87 -0.9 1,198,381 - HMPREF1058_RS08295 0.87 -0.4 1,198,550 - -0.3 1,198,555 - -0.9 1,198,633 - -0.9 1,198,764 - -0.1 1,198,764 - +0.8 1,198,871 + -0.9 1,198,940 + HMPREF1058_RS08290 0.14 -0.3 1,198,940 + HMPREF1058_RS08290 0.14 +0.0 1,199,022 + HMPREF1058_RS08290 0.32 -0.3 1,199,213 + HMPREF1058_RS08290 0.73 +0.0 1,199,215 + HMPREF1058_RS08290 0.73 -0.6 1,199,215 + HMPREF1058_RS08290 0.73 +0.2 1,199,294 + -0.7 1,199,324 + -0.1 1,199,337 + -0.5
Or see this region's nucleotide sequence