Experiment: Sucrose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS07450 and HMPREF1058_RS07445 are separated by 89 nucleotides HMPREF1058_RS07445 and HMPREF1058_RS07440 are separated by 117 nucleotides HMPREF1058_RS07440 and HMPREF1058_RS07435 are separated by 202 nucleotides HMPREF1058_RS07435 and HMPREF1058_RS07430 are separated by 311 nucleotides
HMPREF1058_RS07450: HMPREF1058_RS07450 - LPS-assembly protein LptD, at 220,960 to 223,695
_RS07450
HMPREF1058_RS07445: HMPREF1058_RS07445 - uracil-DNA glycosylase, at 223,785 to 224,447
_RS07445
HMPREF1058_RS07440: HMPREF1058_RS07440 - aspartate--ammonia ligase, at 224,565 to 225,605
_RS07440
HMPREF1058_RS07435: HMPREF1058_RS07435 - tRNA-Arg, at 225,808 to 225,879
_RS07435
HMPREF1058_RS07430: HMPREF1058_RS07430 - TonB-dependent receptor, at 226,191 to 229,466
_RS07430
Position (kb)
224
225
226 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 223.694 kb on - strand at 223.780 kb on + strand at 223.781 kb on - strand at 223.867 kb on + strand, within HMPREF1058_RS07445 at 223.867 kb on + strand, within HMPREF1058_RS07445 at 223.968 kb on + strand, within HMPREF1058_RS07445 at 223.969 kb on - strand, within HMPREF1058_RS07445 at 223.974 kb on - strand, within HMPREF1058_RS07445 at 224.016 kb on - strand, within HMPREF1058_RS07445 at 224.088 kb on + strand, within HMPREF1058_RS07445 at 224.088 kb on + strand, within HMPREF1058_RS07445 at 224.172 kb on - strand, within HMPREF1058_RS07445 at 224.316 kb on - strand, within HMPREF1058_RS07445 at 224.336 kb on + strand, within HMPREF1058_RS07445 at 224.361 kb on + strand, within HMPREF1058_RS07445 at 224.433 kb on + strand at 224.434 kb on - strand at 224.460 kb on - strand at 224.509 kb on - strand at 224.560 kb on + strand at 224.563 kb on - strand at 224.621 kb on + strand at 224.640 kb on + strand at 224.686 kb on - strand, within HMPREF1058_RS07440 at 224.686 kb on - strand, within HMPREF1058_RS07440 at 224.860 kb on - strand, within HMPREF1058_RS07440 at 224.910 kb on - strand at 224.972 kb on + strand, within HMPREF1058_RS07440 at 224.977 kb on - strand, within HMPREF1058_RS07440 at 224.977 kb on - strand, within HMPREF1058_RS07440 at 224.981 kb on + strand, within HMPREF1058_RS07440 at 224.982 kb on - strand, within HMPREF1058_RS07440 at 224.985 kb on + strand, within HMPREF1058_RS07440 at 225.044 kb on - strand, within HMPREF1058_RS07440 at 225.044 kb on - strand at 225.123 kb on + strand, within HMPREF1058_RS07440 at 225.128 kb on + strand, within HMPREF1058_RS07440 at 225.129 kb on - strand, within HMPREF1058_RS07440 at 225.159 kb on - strand, within HMPREF1058_RS07440 at 225.168 kb on + strand, within HMPREF1058_RS07440 at 225.168 kb on + strand, within HMPREF1058_RS07440 at 225.169 kb on - strand, within HMPREF1058_RS07440 at 225.234 kb on + strand, within HMPREF1058_RS07440 at 225.239 kb on - strand, within HMPREF1058_RS07440 at 225.239 kb on - strand, within HMPREF1058_RS07440 at 225.239 kb on - strand, within HMPREF1058_RS07440 at 225.240 kb on + strand, within HMPREF1058_RS07440 at 225.240 kb on + strand, within HMPREF1058_RS07440 at 225.317 kb on + strand, within HMPREF1058_RS07440 at 225.322 kb on + strand, within HMPREF1058_RS07440 at 225.395 kb on + strand, within HMPREF1058_RS07440 at 225.493 kb on - strand at 225.577 kb on + strand at 225.620 kb on - strand at 225.704 kb on + strand at 225.928 kb on + strand at 225.930 kb on + strand at 226.042 kb on + strand at 226.062 kb on - strand at 226.083 kb on - strand at 226.216 kb on + strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.365 kb on - strand at 226.365 kb on - strand at 226.405 kb on - strand at 226.406 kb on + strand at 226.407 kb on - strand at 226.408 kb on + strand at 226.409 kb on - strand at 226.452 kb on + strand at 226.570 kb on + strand, within HMPREF1058_RS07430 at 226.573 kb on + strand, within HMPREF1058_RS07430 at 226.586 kb on + strand, within HMPREF1058_RS07430
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose 10 mM (C) remove 223,694 - -0.1 223,780 + -0.9 223,781 - -0.4 223,867 + HMPREF1058_RS07445 0.12 -0.8 223,867 + HMPREF1058_RS07445 0.12 -0.0 223,968 + HMPREF1058_RS07445 0.28 -1.6 223,969 - HMPREF1058_RS07445 0.28 -0.6 223,974 - HMPREF1058_RS07445 0.29 -0.8 224,016 - HMPREF1058_RS07445 0.35 +1.4 224,088 + HMPREF1058_RS07445 0.46 -0.0 224,088 + HMPREF1058_RS07445 0.46 +0.2 224,172 - HMPREF1058_RS07445 0.58 -1.4 224,316 - HMPREF1058_RS07445 0.80 +0.5 224,336 + HMPREF1058_RS07445 0.83 +0.2 224,361 + HMPREF1058_RS07445 0.87 +1.0 224,433 + +0.5 224,434 - -0.0 224,460 - +0.7 224,509 - +1.5 224,560 + -0.9 224,563 - +1.3 224,621 + -1.0 224,640 + -1.7 224,686 - HMPREF1058_RS07440 0.12 -3.1 224,686 - HMPREF1058_RS07440 0.12 -1.4 224,860 - HMPREF1058_RS07440 0.28 -0.4 224,910 - -1.1 224,972 + HMPREF1058_RS07440 0.39 -4.4 224,977 - HMPREF1058_RS07440 0.40 -3.3 224,977 - HMPREF1058_RS07440 0.40 -1.5 224,981 + HMPREF1058_RS07440 0.40 -2.5 224,982 - HMPREF1058_RS07440 0.40 -1.1 224,985 + HMPREF1058_RS07440 0.40 -1.3 225,044 - HMPREF1058_RS07440 0.46 -1.1 225,044 - -0.4 225,123 + HMPREF1058_RS07440 0.54 -1.8 225,128 + HMPREF1058_RS07440 0.54 -2.3 225,129 - HMPREF1058_RS07440 0.54 -0.8 225,159 - HMPREF1058_RS07440 0.57 -3.5 225,168 + HMPREF1058_RS07440 0.58 -0.6 225,168 + HMPREF1058_RS07440 0.58 +1.7 225,169 - HMPREF1058_RS07440 0.58 -1.0 225,234 + HMPREF1058_RS07440 0.64 -3.2 225,239 - HMPREF1058_RS07440 0.65 -1.9 225,239 - HMPREF1058_RS07440 0.65 -2.2 225,239 - HMPREF1058_RS07440 0.65 -2.3 225,240 + HMPREF1058_RS07440 0.65 -0.4 225,240 + HMPREF1058_RS07440 0.65 -2.2 225,317 + HMPREF1058_RS07440 0.72 -0.2 225,322 + HMPREF1058_RS07440 0.73 -3.6 225,395 + HMPREF1058_RS07440 0.80 -1.0 225,493 - -1.6 225,577 + -0.5 225,620 - -1.1 225,704 + -0.1 225,928 + +0.7 225,930 + +0.6 226,042 + +0.5 226,062 - -0.0 226,083 - -1.1 226,216 + +1.3 226,276 + +0.8 226,276 + -0.6 226,276 + +0.9 226,365 - +0.7 226,365 - +0.3 226,405 - -0.9 226,406 + -0.0 226,407 - +0.7 226,408 + +0.8 226,409 - +0.1 226,452 + -1.0 226,570 + HMPREF1058_RS07430 0.12 +0.6 226,573 + HMPREF1058_RS07430 0.12 +0.3 226,586 + HMPREF1058_RS07430 0.12 +0.9
Or see this region's nucleotide sequence