Experiment: Sucrose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS07095 and HMPREF1058_RS07090 are separated by 29 nucleotides HMPREF1058_RS07090 and HMPREF1058_RS07085 are separated by 2 nucleotides HMPREF1058_RS07085 and HMPREF1058_RS07080 are separated by 77 nucleotides HMPREF1058_RS07080 and HMPREF1058_RS07075 are separated by 210 nucleotides
HMPREF1058_RS07095: HMPREF1058_RS07095 - PD40 domain-containing protein, at 317,974 to 321,234
_RS07095
HMPREF1058_RS07090: HMPREF1058_RS07090 - hypothetical protein, at 321,264 to 321,530
_RS07090
HMPREF1058_RS07085: HMPREF1058_RS07085 - phage holin family protein, at 321,533 to 321,889
_RS07085
HMPREF1058_RS07080: HMPREF1058_RS07080 - YtxH domain-containing protein, at 321,967 to 322,164
_RS07080
HMPREF1058_RS07075: HMPREF1058_RS07075 - SPOR domain-containing protein, at 322,375 to 322,872
_RS07075
Position (kb) 321
322 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 320.377 kb on + strand, within HMPREF1058_RS07095 at 320.410 kb on + strand, within HMPREF1058_RS07095 at 320.411 kb on - strand, within HMPREF1058_RS07095 at 320.411 kb on - strand, within HMPREF1058_RS07095 at 320.481 kb on + strand, within HMPREF1058_RS07095 at 320.534 kb on - strand, within HMPREF1058_RS07095 at 320.537 kb on + strand, within HMPREF1058_RS07095 at 320.709 kb on - strand, within HMPREF1058_RS07095 at 320.742 kb on + strand, within HMPREF1058_RS07095 at 320.743 kb on - strand, within HMPREF1058_RS07095 at 320.803 kb on + strand, within HMPREF1058_RS07095 at 320.804 kb on - strand, within HMPREF1058_RS07095 at 320.854 kb on + strand, within HMPREF1058_RS07095 at 320.892 kb on - strand, within HMPREF1058_RS07095 at 320.923 kb on - strand at 320.974 kb on - strand at 321.057 kb on + strand at 321.081 kb on + strand at 321.111 kb on - strand at 321.182 kb on + strand at 321.183 kb on - strand at 321.221 kb on - strand at 321.258 kb on - strand at 321.260 kb on + strand at 321.265 kb on + strand at 321.287 kb on - strand at 321.351 kb on - strand, within HMPREF1058_RS07090 at 321.448 kb on - strand, within HMPREF1058_RS07090 at 321.661 kb on + strand, within HMPREF1058_RS07085 at 321.671 kb on + strand, within HMPREF1058_RS07085 at 321.671 kb on + strand, within HMPREF1058_RS07085 at 321.690 kb on + strand, within HMPREF1058_RS07085 at 321.700 kb on + strand, within HMPREF1058_RS07085 at 321.700 kb on + strand, within HMPREF1058_RS07085 at 321.711 kb on + strand, within HMPREF1058_RS07085 at 321.711 kb on + strand, within HMPREF1058_RS07085 at 321.712 kb on - strand, within HMPREF1058_RS07085 at 321.725 kb on - strand, within HMPREF1058_RS07085 at 321.735 kb on - strand, within HMPREF1058_RS07085 at 321.775 kb on + strand, within HMPREF1058_RS07085 at 321.775 kb on + strand, within HMPREF1058_RS07085 at 321.775 kb on + strand, within HMPREF1058_RS07085 at 321.792 kb on - strand, within HMPREF1058_RS07085 at 321.804 kb on - strand, within HMPREF1058_RS07085 at 321.804 kb on - strand, within HMPREF1058_RS07085 at 321.862 kb on + strand at 321.863 kb on - strand at 321.947 kb on + strand at 321.947 kb on + strand at 321.947 kb on + strand at 322.024 kb on + strand, within HMPREF1058_RS07080 at 322.025 kb on - strand, within HMPREF1058_RS07080 at 322.025 kb on - strand, within HMPREF1058_RS07080 at 322.042 kb on - strand, within HMPREF1058_RS07080 at 322.046 kb on - strand, within HMPREF1058_RS07080 at 322.149 kb on + strand at 322.149 kb on + strand at 322.149 kb on + strand at 322.305 kb on + strand at 322.307 kb on + strand at 322.327 kb on + strand at 322.327 kb on + strand at 322.329 kb on + strand at 322.348 kb on + strand at 322.349 kb on - strand at 322.352 kb on - strand at 322.352 kb on - strand at 322.386 kb on - strand at 322.454 kb on - strand, within HMPREF1058_RS07075 at 322.465 kb on + strand, within HMPREF1058_RS07075 at 322.465 kb on + strand, within HMPREF1058_RS07075
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose 10 mM (C) remove 320,377 + HMPREF1058_RS07095 0.74 +0.5 320,410 + HMPREF1058_RS07095 0.75 +0.0 320,411 - HMPREF1058_RS07095 0.75 -1.9 320,411 - HMPREF1058_RS07095 0.75 -2.0 320,481 + HMPREF1058_RS07095 0.77 -0.1 320,534 - HMPREF1058_RS07095 0.79 -0.4 320,537 + HMPREF1058_RS07095 0.79 +0.3 320,709 - HMPREF1058_RS07095 0.84 -1.6 320,742 + HMPREF1058_RS07095 0.85 -0.8 320,743 - HMPREF1058_RS07095 0.85 -0.1 320,803 + HMPREF1058_RS07095 0.87 -1.4 320,804 - HMPREF1058_RS07095 0.87 -0.4 320,854 + HMPREF1058_RS07095 0.88 +0.7 320,892 - HMPREF1058_RS07095 0.89 -0.3 320,923 - -0.2 320,974 - -0.8 321,057 + -0.7 321,081 + +0.9 321,111 - -0.2 321,182 + +0.9 321,183 - -0.9 321,221 - -3.9 321,258 - -1.4 321,260 + +0.0 321,265 + -0.3 321,287 - -1.4 321,351 - HMPREF1058_RS07090 0.33 -1.9 321,448 - HMPREF1058_RS07090 0.69 -1.9 321,661 + HMPREF1058_RS07085 0.36 -0.0 321,671 + HMPREF1058_RS07085 0.39 -0.9 321,671 + HMPREF1058_RS07085 0.39 +0.6 321,690 + HMPREF1058_RS07085 0.44 +1.1 321,700 + HMPREF1058_RS07085 0.47 -0.3 321,700 + HMPREF1058_RS07085 0.47 +0.9 321,711 + HMPREF1058_RS07085 0.50 +1.1 321,711 + HMPREF1058_RS07085 0.50 -0.2 321,712 - HMPREF1058_RS07085 0.50 -1.4 321,725 - HMPREF1058_RS07085 0.54 -0.9 321,735 - HMPREF1058_RS07085 0.57 -1.1 321,775 + HMPREF1058_RS07085 0.68 +1.8 321,775 + HMPREF1058_RS07085 0.68 -1.6 321,775 + HMPREF1058_RS07085 0.68 +1.6 321,792 - HMPREF1058_RS07085 0.73 +1.6 321,804 - HMPREF1058_RS07085 0.76 -2.5 321,804 - HMPREF1058_RS07085 0.76 -2.7 321,862 + +0.3 321,863 - +0.6 321,947 + -0.2 321,947 + +1.6 321,947 + +1.6 322,024 + HMPREF1058_RS07080 0.29 +0.8 322,025 - HMPREF1058_RS07080 0.29 -1.2 322,025 - HMPREF1058_RS07080 0.29 +0.6 322,042 - HMPREF1058_RS07080 0.38 -0.7 322,046 - HMPREF1058_RS07080 0.40 -0.7 322,149 + +0.3 322,149 + +0.5 322,149 + -0.1 322,305 + -0.6 322,307 + -1.1 322,327 + -3.6 322,327 + -0.1 322,329 + -1.3 322,348 + -1.1 322,349 - -0.2 322,352 - +0.9 322,352 - +0.6 322,386 - -1.5 322,454 - HMPREF1058_RS07075 0.16 -2.0 322,465 + HMPREF1058_RS07075 0.18 -2.7 322,465 + HMPREF1058_RS07075 0.18 -1.2
Or see this region's nucleotide sequence