Experiment: Sucrose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06170 and HMPREF1058_RS06165 are separated by 41 nucleotides HMPREF1058_RS06165 and HMPREF1058_RS06160 overlap by 1 nucleotides HMPREF1058_RS06160 and HMPREF1058_RS06155 are separated by 264 nucleotides HMPREF1058_RS06155 and HMPREF1058_RS06150 are separated by 30 nucleotides HMPREF1058_RS06150 and HMPREF1058_RS06145 are separated by 29 nucleotides HMPREF1058_RS06145 and HMPREF1058_RS06140 are separated by 257 nucleotides
HMPREF1058_RS06170: HMPREF1058_RS06170 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 542,592 to 543,311
_RS06170
HMPREF1058_RS06165: HMPREF1058_RS06165 - imidazole glycerol phosphate synthase subunit HisH, at 543,353 to 543,943
_RS06165
HMPREF1058_RS06160: HMPREF1058_RS06160 - formyltetrahydrofolate deformylase, at 543,943 to 544,800
_RS06160
HMPREF1058_RS06155: HMPREF1058_RS06155 - tRNA-Asp, at 545,065 to 545,138
_RS06155
HMPREF1058_RS06150: HMPREF1058_RS06150 - tRNA-Asp, at 545,169 to 545,242
_RS06150
HMPREF1058_RS06145: HMPREF1058_RS06145 - tRNA-Asp, at 545,272 to 545,345
_RS06145
HMPREF1058_RS06140: HMPREF1058_RS06140 - phage integrase SAM-like domain-containing protein, at 545,603 to 546,238
_RS06140
Position (kb) 543
544
545 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 543.010 kb on - strand, within HMPREF1058_RS06170 at 543.228 kb on + strand, within HMPREF1058_RS06170 at 543.229 kb on - strand, within HMPREF1058_RS06170 at 543.244 kb on + strand at 543.300 kb on + strand at 543.301 kb on - strand at 543.301 kb on - strand at 543.315 kb on + strand at 543.316 kb on - strand at 543.328 kb on - strand at 543.328 kb on - strand at 543.335 kb on + strand at 543.340 kb on - strand at 543.340 kb on - strand at 543.342 kb on - strand at 543.467 kb on - strand, within HMPREF1058_RS06165 at 543.467 kb on - strand, within HMPREF1058_RS06165 at 543.507 kb on + strand, within HMPREF1058_RS06165 at 543.508 kb on - strand, within HMPREF1058_RS06165 at 543.519 kb on - strand, within HMPREF1058_RS06165 at 543.519 kb on - strand, within HMPREF1058_RS06165 at 543.638 kb on + strand, within HMPREF1058_RS06165 at 543.638 kb on + strand, within HMPREF1058_RS06165 at 543.722 kb on + strand, within HMPREF1058_RS06165 at 543.726 kb on - strand, within HMPREF1058_RS06165 at 543.737 kb on + strand, within HMPREF1058_RS06165 at 543.737 kb on + strand, within HMPREF1058_RS06165 at 543.746 kb on - strand, within HMPREF1058_RS06165 at 543.839 kb on + strand, within HMPREF1058_RS06165 at 543.840 kb on - strand, within HMPREF1058_RS06165 at 543.903 kb on + strand at 543.954 kb on + strand at 543.955 kb on - strand at 543.972 kb on - strand at 543.993 kb on + strand at 543.993 kb on + strand at 544.048 kb on + strand, within HMPREF1058_RS06160 at 544.087 kb on + strand, within HMPREF1058_RS06160 at 544.088 kb on - strand, within HMPREF1058_RS06160 at 544.225 kb on + strand, within HMPREF1058_RS06160 at 544.282 kb on - strand, within HMPREF1058_RS06160 at 544.431 kb on + strand, within HMPREF1058_RS06160 at 544.435 kb on + strand, within HMPREF1058_RS06160 at 544.435 kb on + strand, within HMPREF1058_RS06160 at 544.488 kb on + strand, within HMPREF1058_RS06160 at 544.541 kb on - strand, within HMPREF1058_RS06160 at 544.586 kb on + strand, within HMPREF1058_RS06160 at 544.602 kb on - strand, within HMPREF1058_RS06160 at 544.639 kb on + strand, within HMPREF1058_RS06160 at 544.689 kb on - strand, within HMPREF1058_RS06160 at 544.726 kb on - strand at 544.795 kb on - strand at 545.091 kb on - strand, within HMPREF1058_RS06155 at 545.280 kb on - strand, within HMPREF1058_RS06145 at 545.393 kb on + strand at 545.445 kb on + strand at 545.446 kb on - strand at 545.484 kb on - strand at 545.485 kb on + strand at 545.554 kb on - strand at 545.574 kb on - strand at 545.588 kb on - strand at 545.623 kb on - strand at 545.623 kb on - strand at 545.792 kb on + strand, within HMPREF1058_RS06140 at 545.792 kb on + strand, within HMPREF1058_RS06140 at 545.794 kb on + strand, within HMPREF1058_RS06140 at 545.795 kb on - strand, within HMPREF1058_RS06140
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose 10 mM (C) remove 543,010 - HMPREF1058_RS06170 0.58 -4.4 543,228 + HMPREF1058_RS06170 0.88 -3.0 543,229 - HMPREF1058_RS06170 0.88 -3.8 543,244 + -2.3 543,300 + -1.9 543,301 - -3.5 543,301 - -3.9 543,315 + -2.5 543,316 - -3.7 543,328 - -1.8 543,328 - -2.3 543,335 + -2.7 543,340 - -3.1 543,340 - -2.6 543,342 - -2.5 543,467 - HMPREF1058_RS06165 0.19 -3.7 543,467 - HMPREF1058_RS06165 0.19 -1.5 543,507 + HMPREF1058_RS06165 0.26 -1.1 543,508 - HMPREF1058_RS06165 0.26 -3.6 543,519 - HMPREF1058_RS06165 0.28 -4.4 543,519 - HMPREF1058_RS06165 0.28 -4.7 543,638 + HMPREF1058_RS06165 0.48 -3.7 543,638 + HMPREF1058_RS06165 0.48 -3.1 543,722 + HMPREF1058_RS06165 0.62 -3.5 543,726 - HMPREF1058_RS06165 0.63 -2.0 543,737 + HMPREF1058_RS06165 0.65 -3.9 543,737 + HMPREF1058_RS06165 0.65 -3.3 543,746 - HMPREF1058_RS06165 0.66 -2.6 543,839 + HMPREF1058_RS06165 0.82 -0.1 543,840 - HMPREF1058_RS06165 0.82 -3.7 543,903 + -3.3 543,954 + -5.7 543,955 - -1.9 543,972 - -1.7 543,993 + -3.4 543,993 + -3.9 544,048 + HMPREF1058_RS06160 0.12 -3.1 544,087 + HMPREF1058_RS06160 0.17 -3.1 544,088 - HMPREF1058_RS06160 0.17 -2.0 544,225 + HMPREF1058_RS06160 0.33 -3.2 544,282 - HMPREF1058_RS06160 0.40 -1.1 544,431 + HMPREF1058_RS06160 0.57 -3.2 544,435 + HMPREF1058_RS06160 0.57 -3.1 544,435 + HMPREF1058_RS06160 0.57 -5.1 544,488 + HMPREF1058_RS06160 0.64 -2.6 544,541 - HMPREF1058_RS06160 0.70 -1.7 544,586 + HMPREF1058_RS06160 0.75 -3.7 544,602 - HMPREF1058_RS06160 0.77 -3.6 544,639 + HMPREF1058_RS06160 0.81 -1.9 544,689 - HMPREF1058_RS06160 0.87 -3.7 544,726 - -2.9 544,795 - -3.2 545,091 - HMPREF1058_RS06155 0.35 +4.0 545,280 - HMPREF1058_RS06145 0.11 +0.2 545,393 + -1.8 545,445 + +0.2 545,446 - -0.1 545,484 - +0.3 545,485 + +0.4 545,554 - +1.2 545,574 - -0.8 545,588 - -0.1 545,623 - -0.3 545,623 - +3.0 545,792 + HMPREF1058_RS06140 0.30 -0.4 545,792 + HMPREF1058_RS06140 0.30 -0.1 545,794 + HMPREF1058_RS06140 0.30 +0.5 545,795 - HMPREF1058_RS06140 0.30 +0.3
Or see this region's nucleotide sequence