Experiment: D-Glucosamine Hydrochloride 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06425 and HMPREF1058_RS06420 are separated by 154 nucleotides HMPREF1058_RS06420 and HMPREF1058_RS06415 are separated by 39 nucleotides HMPREF1058_RS06415 and HMPREF1058_RS06410 are separated by 83 nucleotides HMPREF1058_RS06410 and HMPREF1058_RS06405 are separated by 131 nucleotides
HMPREF1058_RS06425: HMPREF1058_RS06425 - winged helix-turn-helix domain-containing protein, at 481,709 to 481,912
_RS06425
HMPREF1058_RS06420: HMPREF1058_RS06420 - type II toxin-antitoxin system HicA family toxin, at 482,067 to 482,255
_RS06420
HMPREF1058_RS06415: HMPREF1058_RS06415 - antitoxin HicB, at 482,295 to 482,687
_RS06415
HMPREF1058_RS06410: HMPREF1058_RS06410 - elongation factor 4, at 482,771 to 484,552
_RS06410
HMPREF1058_RS06405: HMPREF1058_RS06405 - Na+/H+ antiporter NhaA, at 484,684 to 486,024
_RS06405
Position (kb) 482
483
484
485 Strain fitness (log2 ratio) -2
-1
0
1
2 at 481.861 kb on + strand, within HMPREF1058_RS06425 at 481.861 kb on + strand, within HMPREF1058_RS06425 at 481.911 kb on - strand at 481.987 kb on + strand at 481.999 kb on - strand at 482.074 kb on - strand at 482.122 kb on + strand, within HMPREF1058_RS06420 at 482.122 kb on + strand, within HMPREF1058_RS06420 at 482.220 kb on + strand, within HMPREF1058_RS06420 at 482.220 kb on + strand, within HMPREF1058_RS06420 at 482.220 kb on + strand, within HMPREF1058_RS06420 at 482.220 kb on + strand, within HMPREF1058_RS06420 at 482.221 kb on - strand, within HMPREF1058_RS06420 at 482.221 kb on - strand, within HMPREF1058_RS06420 at 482.519 kb on + strand, within HMPREF1058_RS06415 at 482.674 kb on + strand at 482.755 kb on + strand at 482.799 kb on + strand at 482.806 kb on + strand at 482.806 kb on + strand at 482.806 kb on + strand at 482.808 kb on + strand at 482.808 kb on + strand at 482.918 kb on - strand at 482.918 kb on - strand at 482.973 kb on - strand, within HMPREF1058_RS06410 at 482.977 kb on - strand, within HMPREF1058_RS06410 at 483.232 kb on + strand, within HMPREF1058_RS06410 at 483.455 kb on + strand, within HMPREF1058_RS06410 at 483.532 kb on + strand, within HMPREF1058_RS06410 at 483.664 kb on + strand, within HMPREF1058_RS06410 at 483.841 kb on + strand, within HMPREF1058_RS06410 at 484.157 kb on + strand, within HMPREF1058_RS06410 at 484.172 kb on + strand, within HMPREF1058_RS06410 at 484.294 kb on + strand, within HMPREF1058_RS06410 at 484.295 kb on - strand, within HMPREF1058_RS06410 at 484.372 kb on + strand, within HMPREF1058_RS06410 at 484.477 kb on + strand at 484.562 kb on - strand at 484.738 kb on + strand at 484.743 kb on + strand at 484.744 kb on - strand at 484.744 kb on - strand at 484.746 kb on + strand at 484.816 kb on - strand at 484.820 kb on - strand, within HMPREF1058_RS06405 at 484.820 kb on - strand, within HMPREF1058_RS06405 at 484.820 kb on - strand, within HMPREF1058_RS06405 at 484.961 kb on + strand, within HMPREF1058_RS06405 at 485.059 kb on + strand, within HMPREF1058_RS06405 at 485.063 kb on - strand, within HMPREF1058_RS06405 at 485.347 kb on - strand at 485.365 kb on - strand, within HMPREF1058_RS06405 at 485.450 kb on - strand, within HMPREF1058_RS06405 at 485.460 kb on + strand, within HMPREF1058_RS06405 at 485.460 kb on + strand, within HMPREF1058_RS06405 at 485.460 kb on + strand, within HMPREF1058_RS06405 at 485.460 kb on + strand, within HMPREF1058_RS06405 at 485.460 kb on + strand, within HMPREF1058_RS06405 at 485.461 kb on - strand, within HMPREF1058_RS06405 at 485.461 kb on - strand, within HMPREF1058_RS06405 at 485.464 kb on + strand, within HMPREF1058_RS06405 at 485.465 kb on - strand, within HMPREF1058_RS06405 at 485.465 kb on - strand, within HMPREF1058_RS06405
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride 10 mM (C) remove 481,861 + HMPREF1058_RS06425 0.75 -0.3 481,861 + HMPREF1058_RS06425 0.75 +1.0 481,911 - +0.8 481,987 + +0.6 481,999 - -0.6 482,074 - -0.6 482,122 + HMPREF1058_RS06420 0.29 -0.5 482,122 + HMPREF1058_RS06420 0.29 -0.5 482,220 + HMPREF1058_RS06420 0.81 +0.2 482,220 + HMPREF1058_RS06420 0.81 +0.7 482,220 + HMPREF1058_RS06420 0.81 -0.2 482,220 + HMPREF1058_RS06420 0.81 +2.1 482,221 - HMPREF1058_RS06420 0.81 -0.5 482,221 - HMPREF1058_RS06420 0.81 -0.0 482,519 + HMPREF1058_RS06415 0.57 -0.1 482,674 + -1.6 482,755 + -0.8 482,799 + -0.1 482,806 + +1.4 482,806 + -0.8 482,806 + +0.9 482,808 + +0.7 482,808 + -1.6 482,918 - -0.9 482,918 - +0.7 482,973 - HMPREF1058_RS06410 0.11 -0.1 482,977 - HMPREF1058_RS06410 0.12 +0.7 483,232 + HMPREF1058_RS06410 0.26 +0.7 483,455 + HMPREF1058_RS06410 0.38 +1.0 483,532 + HMPREF1058_RS06410 0.43 +0.7 483,664 + HMPREF1058_RS06410 0.50 -1.8 483,841 + HMPREF1058_RS06410 0.60 -0.4 484,157 + HMPREF1058_RS06410 0.78 +0.2 484,172 + HMPREF1058_RS06410 0.79 -0.8 484,294 + HMPREF1058_RS06410 0.85 -0.1 484,295 - HMPREF1058_RS06410 0.86 +2.2 484,372 + HMPREF1058_RS06410 0.90 +1.2 484,477 + +0.1 484,562 - +1.0 484,738 + -1.9 484,743 + +1.2 484,744 - +0.5 484,744 - -1.0 484,746 + +1.0 484,816 - +0.9 484,820 - HMPREF1058_RS06405 0.10 -1.6 484,820 - HMPREF1058_RS06405 0.10 -0.9 484,820 - HMPREF1058_RS06405 0.10 -0.0 484,961 + HMPREF1058_RS06405 0.21 +2.2 485,059 + HMPREF1058_RS06405 0.28 +0.2 485,063 - HMPREF1058_RS06405 0.28 -0.1 485,347 - +0.7 485,365 - HMPREF1058_RS06405 0.51 -1.5 485,450 - HMPREF1058_RS06405 0.57 +0.4 485,460 + HMPREF1058_RS06405 0.58 +0.2 485,460 + HMPREF1058_RS06405 0.58 -0.2 485,460 + HMPREF1058_RS06405 0.58 -0.0 485,460 + HMPREF1058_RS06405 0.58 +0.1 485,460 + HMPREF1058_RS06405 0.58 -1.1 485,461 - HMPREF1058_RS06405 0.58 +0.5 485,461 - HMPREF1058_RS06405 0.58 -0.1 485,464 + HMPREF1058_RS06405 0.58 -0.2 485,465 - HMPREF1058_RS06405 0.58 -0.5 485,465 - HMPREF1058_RS06405 0.58 -0.5
Or see this region's nucleotide sequence