Experiment: D-Mannose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS15890 and HMPREF1058_RS15885 are separated by 89 nucleotides HMPREF1058_RS15885 and HMPREF1058_RS15880 are separated by 3 nucleotides
HMPREF1058_RS15890: HMPREF1058_RS15890 - NAD(+) synthase, at 811,810 to 813,735
_RS15890
HMPREF1058_RS15885: HMPREF1058_RS15885 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 813,825 to 814,943
_RS15885
HMPREF1058_RS15880: HMPREF1058_RS15880 - histidinol-phosphate transaminase, at 814,947 to 815,987
_RS15880
Position (kb) 813
814
815 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 813.743 kb on - strand at 813.778 kb on - strand at 813.800 kb on - strand at 813.800 kb on - strand at 813.804 kb on + strand at 813.831 kb on - strand at 813.831 kb on - strand at 813.831 kb on - strand at 813.850 kb on - strand at 813.921 kb on + strand at 813.922 kb on - strand at 813.926 kb on + strand at 813.960 kb on + strand, within HMPREF1058_RS15885 at 814.172 kb on + strand, within HMPREF1058_RS15885 at 814.172 kb on + strand, within HMPREF1058_RS15885 at 814.172 kb on + strand at 814.205 kb on + strand, within HMPREF1058_RS15885 at 814.208 kb on + strand, within HMPREF1058_RS15885 at 814.209 kb on - strand, within HMPREF1058_RS15885 at 814.240 kb on - strand, within HMPREF1058_RS15885 at 814.357 kb on + strand, within HMPREF1058_RS15885 at 814.358 kb on - strand, within HMPREF1058_RS15885 at 814.358 kb on - strand, within HMPREF1058_RS15885 at 814.358 kb on - strand, within HMPREF1058_RS15885 at 814.361 kb on + strand, within HMPREF1058_RS15885 at 814.397 kb on + strand, within HMPREF1058_RS15885 at 814.397 kb on + strand, within HMPREF1058_RS15885 at 814.453 kb on - strand, within HMPREF1058_RS15885 at 814.494 kb on - strand, within HMPREF1058_RS15885 at 814.840 kb on - strand at 814.840 kb on - strand at 814.910 kb on + strand at 814.911 kb on - strand at 814.926 kb on - strand at 814.944 kb on - strand at 814.957 kb on + strand at 815.040 kb on - strand at 815.059 kb on - strand, within HMPREF1058_RS15880 at 815.074 kb on - strand, within HMPREF1058_RS15880 at 815.080 kb on + strand, within HMPREF1058_RS15880 at 815.088 kb on + strand, within HMPREF1058_RS15880 at 815.088 kb on + strand, within HMPREF1058_RS15880 at 815.112 kb on - strand, within HMPREF1058_RS15880 at 815.230 kb on + strand, within HMPREF1058_RS15880 at 815.294 kb on + strand, within HMPREF1058_RS15880 at 815.295 kb on - strand, within HMPREF1058_RS15880 at 815.295 kb on - strand, within HMPREF1058_RS15880 at 815.310 kb on - strand, within HMPREF1058_RS15880 at 815.405 kb on - strand, within HMPREF1058_RS15880 at 815.408 kb on + strand, within HMPREF1058_RS15880 at 815.463 kb on + strand, within HMPREF1058_RS15880 at 815.671 kb on + strand, within HMPREF1058_RS15880 at 815.672 kb on - strand, within HMPREF1058_RS15880 at 815.672 kb on - strand, within HMPREF1058_RS15880 at 815.675 kb on + strand, within HMPREF1058_RS15880 at 815.675 kb on + strand, within HMPREF1058_RS15880 at 815.676 kb on - strand, within HMPREF1058_RS15880 at 815.676 kb on - strand, within HMPREF1058_RS15880 at 815.676 kb on - strand, within HMPREF1058_RS15880 at 815.684 kb on + strand, within HMPREF1058_RS15880 at 815.700 kb on + strand, within HMPREF1058_RS15880 at 815.813 kb on + strand, within HMPREF1058_RS15880 at 815.814 kb on - strand, within HMPREF1058_RS15880 at 815.835 kb on - strand, within HMPREF1058_RS15880 at 815.835 kb on - strand, within HMPREF1058_RS15880 at 815.855 kb on + strand, within HMPREF1058_RS15880 at 815.856 kb on - strand, within HMPREF1058_RS15880 at 815.861 kb on + strand, within HMPREF1058_RS15880 at 815.862 kb on - strand, within HMPREF1058_RS15880 at 815.912 kb on + strand at 815.935 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose 10 mM (C) remove 813,743 - -1.1 813,778 - -2.1 813,800 - +2.2 813,800 - -0.9 813,804 + -1.3 813,831 - -5.9 813,831 - -4.8 813,831 - -1.3 813,850 - -2.7 813,921 + -3.7 813,922 - -3.5 813,926 + -0.4 813,960 + HMPREF1058_RS15885 0.12 -3.5 814,172 + HMPREF1058_RS15885 0.31 -5.5 814,172 + HMPREF1058_RS15885 0.31 -2.3 814,172 + +0.9 814,205 + HMPREF1058_RS15885 0.34 -0.9 814,208 + HMPREF1058_RS15885 0.34 -4.1 814,209 - HMPREF1058_RS15885 0.34 -4.7 814,240 - HMPREF1058_RS15885 0.37 -1.9 814,357 + HMPREF1058_RS15885 0.48 -3.6 814,358 - HMPREF1058_RS15885 0.48 -6.7 814,358 - HMPREF1058_RS15885 0.48 -4.3 814,358 - HMPREF1058_RS15885 0.48 -4.9 814,361 + HMPREF1058_RS15885 0.48 -3.0 814,397 + HMPREF1058_RS15885 0.51 -4.4 814,397 + HMPREF1058_RS15885 0.51 -3.7 814,453 - HMPREF1058_RS15885 0.56 -3.3 814,494 - HMPREF1058_RS15885 0.60 -3.2 814,840 - -5.0 814,840 - -1.4 814,910 + -5.8 814,911 - -1.7 814,926 - -4.6 814,944 - -3.1 814,957 + -3.4 815,040 - -3.1 815,059 - HMPREF1058_RS15880 0.11 -4.3 815,074 - HMPREF1058_RS15880 0.12 -3.4 815,080 + HMPREF1058_RS15880 0.13 -2.5 815,088 + HMPREF1058_RS15880 0.14 -3.0 815,088 + HMPREF1058_RS15880 0.14 -5.8 815,112 - HMPREF1058_RS15880 0.16 -4.7 815,230 + HMPREF1058_RS15880 0.27 -2.5 815,294 + HMPREF1058_RS15880 0.33 -2.5 815,295 - HMPREF1058_RS15880 0.33 -5.9 815,295 - HMPREF1058_RS15880 0.33 -2.9 815,310 - HMPREF1058_RS15880 0.35 -4.6 815,405 - HMPREF1058_RS15880 0.44 -3.1 815,408 + HMPREF1058_RS15880 0.44 -5.5 815,463 + HMPREF1058_RS15880 0.50 -2.4 815,671 + HMPREF1058_RS15880 0.70 -2.5 815,672 - HMPREF1058_RS15880 0.70 -5.3 815,672 - HMPREF1058_RS15880 0.70 -2.9 815,675 + HMPREF1058_RS15880 0.70 -4.7 815,675 + HMPREF1058_RS15880 0.70 -3.9 815,676 - HMPREF1058_RS15880 0.70 -3.4 815,676 - HMPREF1058_RS15880 0.70 -1.4 815,676 - HMPREF1058_RS15880 0.70 -2.8 815,684 + HMPREF1058_RS15880 0.71 -2.9 815,700 + HMPREF1058_RS15880 0.72 -4.3 815,813 + HMPREF1058_RS15880 0.83 -2.1 815,814 - HMPREF1058_RS15880 0.83 -1.7 815,835 - HMPREF1058_RS15880 0.85 -0.9 815,835 - HMPREF1058_RS15880 0.85 -4.4 815,855 + HMPREF1058_RS15880 0.87 -1.4 815,856 - HMPREF1058_RS15880 0.87 -4.0 815,861 + HMPREF1058_RS15880 0.88 -3.4 815,862 - HMPREF1058_RS15880 0.88 -2.2 815,912 + -2.3 815,935 + -3.3
Or see this region's nucleotide sequence