Experiment: AMD2036 coinoculated with AMD2910 on LB to a starting OD of 0.001
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00207 and MPMX20_00208 are separated by 76 nucleotides MPMX20_00208 and MPMX20_00209 are separated by 59 nucleotides MPMX20_00209 and MPMX20_00210 are separated by 3 nucleotides MPMX20_00210 and MPMX20_00211 are separated by 180 nucleotides
MPMX20_00207: MPMX20_00207 - DNA-directed RNA polymerase subunit beta, at 211,136 to 215,164
_00207
MPMX20_00208: MPMX20_00208 - DNA-directed RNA polymerase subunit beta', at 215,241 to 219,464
_00208
MPMX20_00209: MPMX20_00209 - PTS system N,N'-diacetylchitobiose-specific EIIA component, at 219,524 to 219,841
_00209
MPMX20_00210: MPMX20_00210 - PTS system cellobiose-specific EIIB component, at 219,845 to 220,150
_00210
MPMX20_00211: MPMX20_00211 - hypothetical protein, at 220,331 to 221,872
_00211
Position (kb) 215
216
217
218
219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 215.179 kb on + strand at 215.192 kb on + strand at 219.438 kb on + strand at 219.443 kb on + strand at 219.549 kb on - strand at 219.591 kb on + strand, within MPMX20_00209 at 219.604 kb on + strand, within MPMX20_00209 at 219.610 kb on - strand, within MPMX20_00209 at 219.629 kb on - strand, within MPMX20_00209 at 219.665 kb on + strand, within MPMX20_00209 at 219.668 kb on - strand, within MPMX20_00209 at 219.688 kb on + strand, within MPMX20_00209 at 219.783 kb on - strand, within MPMX20_00209 at 219.788 kb on - strand, within MPMX20_00209 at 219.800 kb on - strand, within MPMX20_00209 at 219.893 kb on - strand, within MPMX20_00210 at 219.957 kb on + strand, within MPMX20_00210 at 219.965 kb on - strand, within MPMX20_00210 at 219.998 kb on + strand, within MPMX20_00210 at 220.052 kb on - strand, within MPMX20_00210 at 220.100 kb on - strand, within MPMX20_00210 at 220.156 kb on + strand at 220.314 kb on + strand at 220.358 kb on + strand at 220.366 kb on - strand at 220.372 kb on + strand at 220.380 kb on - strand at 220.397 kb on - strand at 220.444 kb on + strand at 220.444 kb on + strand at 220.452 kb on - strand at 220.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction AMD2036 coinoculated with AMD2910 on LB to a starting OD of 0.001 remove 215,179 + +1.2 215,192 + -1.5 219,438 + -1.2 219,443 + +1.7 219,549 - +2.5 219,591 + MPMX20_00209 0.21 -2.1 219,604 + MPMX20_00209 0.25 -2.9 219,610 - MPMX20_00209 0.27 -0.7 219,629 - MPMX20_00209 0.33 +0.3 219,665 + MPMX20_00209 0.44 -1.9 219,668 - MPMX20_00209 0.45 -3.3 219,688 + MPMX20_00209 0.52 -0.2 219,783 - MPMX20_00209 0.81 -2.2 219,788 - MPMX20_00209 0.83 +2.0 219,800 - MPMX20_00209 0.87 +0.6 219,893 - MPMX20_00210 0.16 -0.0 219,957 + MPMX20_00210 0.37 -2.1 219,965 - MPMX20_00210 0.39 -1.6 219,998 + MPMX20_00210 0.50 +1.5 220,052 - MPMX20_00210 0.68 +3.5 220,100 - MPMX20_00210 0.83 -2.6 220,156 + -3.3 220,314 + -2.4 220,358 + -2.4 220,366 - +3.2 220,372 + +0.4 220,380 - -1.9 220,397 - -0.4 220,444 + -0.9 220,444 + -0.5 220,452 - -2.0 220,457 - -2.1
Or see this region's nucleotide sequence