Experiment: AMD2036 coinoculated with AMD2910 on LB to a starting OD of 0.01
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_02759 and MPMX20_02760 are separated by 55 nucleotides MPMX20_02760 and MPMX20_02761 are separated by 119 nucleotides
MPMX20_02759: MPMX20_02759 - Pyruvate kinase II, at 2,915,163 to 2,916,605
_02759
MPMX20_02760: MPMX20_02760 - Lipid A biosynthesis myristoyltransferase, at 2,916,661 to 2,917,632
_02760
MPMX20_02761: MPMX20_02761 - Murein DD-endopeptidase MepM, at 2,917,752 to 2,919,071
_02761
Position (kb)
2916
2917
2918 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2915.666 kb on + strand, within MPMX20_02759 at 2915.681 kb on - strand, within MPMX20_02759 at 2915.687 kb on - strand, within MPMX20_02759 at 2915.777 kb on - strand, within MPMX20_02759 at 2915.959 kb on + strand, within MPMX20_02759 at 2915.972 kb on - strand, within MPMX20_02759 at 2916.053 kb on - strand, within MPMX20_02759 at 2916.053 kb on - strand, within MPMX20_02759 at 2916.063 kb on - strand, within MPMX20_02759 at 2916.063 kb on - strand, within MPMX20_02759 at 2916.074 kb on + strand, within MPMX20_02759 at 2916.074 kb on + strand, within MPMX20_02759 at 2916.074 kb on + strand, within MPMX20_02759 at 2916.111 kb on - strand, within MPMX20_02759 at 2916.140 kb on - strand, within MPMX20_02759 at 2916.159 kb on - strand, within MPMX20_02759 at 2916.218 kb on - strand, within MPMX20_02759 at 2916.277 kb on + strand, within MPMX20_02759 at 2916.295 kb on - strand, within MPMX20_02759 at 2916.346 kb on + strand, within MPMX20_02759 at 2916.358 kb on + strand, within MPMX20_02759 at 2916.433 kb on + strand, within MPMX20_02759 at 2916.523 kb on - strand at 2916.616 kb on + strand at 2916.631 kb on + strand at 2916.681 kb on - strand at 2917.644 kb on - strand at 2917.809 kb on + strand at 2917.898 kb on - strand, within MPMX20_02761 at 2917.966 kb on + strand, within MPMX20_02761 at 2917.996 kb on - strand, within MPMX20_02761 at 2917.996 kb on - strand, within MPMX20_02761 at 2918.040 kb on - strand, within MPMX20_02761 at 2918.093 kb on + strand, within MPMX20_02761 at 2918.159 kb on + strand, within MPMX20_02761 at 2918.174 kb on + strand, within MPMX20_02761 at 2918.237 kb on + strand, within MPMX20_02761 at 2918.295 kb on + strand, within MPMX20_02761 at 2918.339 kb on - strand, within MPMX20_02761 at 2918.372 kb on - strand, within MPMX20_02761 at 2918.428 kb on + strand, within MPMX20_02761 at 2918.431 kb on - strand, within MPMX20_02761
Per-strain Table
Position Strand Gene LocusTag Fraction AMD2036 coinoculated with AMD2910 on LB to a starting OD of 0.01 remove 2,915,666 + MPMX20_02759 0.35 -1.4 2,915,681 - MPMX20_02759 0.36 +1.9 2,915,687 - MPMX20_02759 0.36 -1.0 2,915,777 - MPMX20_02759 0.43 -0.6 2,915,959 + MPMX20_02759 0.55 -0.5 2,915,972 - MPMX20_02759 0.56 +0.0 2,916,053 - MPMX20_02759 0.62 -1.1 2,916,053 - MPMX20_02759 0.62 -0.6 2,916,063 - MPMX20_02759 0.62 +0.5 2,916,063 - MPMX20_02759 0.62 +0.4 2,916,074 + MPMX20_02759 0.63 +0.2 2,916,074 + MPMX20_02759 0.63 -0.8 2,916,074 + MPMX20_02759 0.63 +0.3 2,916,111 - MPMX20_02759 0.66 -2.0 2,916,140 - MPMX20_02759 0.68 +0.3 2,916,159 - MPMX20_02759 0.69 -0.8 2,916,218 - MPMX20_02759 0.73 -0.6 2,916,277 + MPMX20_02759 0.77 +0.6 2,916,295 - MPMX20_02759 0.78 -0.9 2,916,346 + MPMX20_02759 0.82 +1.8 2,916,358 + MPMX20_02759 0.83 -0.2 2,916,433 + MPMX20_02759 0.88 -0.7 2,916,523 - -1.0 2,916,616 + -0.7 2,916,631 + +0.0 2,916,681 - -2.9 2,917,644 - -0.4 2,917,809 + -0.3 2,917,898 - MPMX20_02761 0.11 -0.0 2,917,966 + MPMX20_02761 0.16 +1.3 2,917,996 - MPMX20_02761 0.18 +0.1 2,917,996 - MPMX20_02761 0.18 -0.3 2,918,040 - MPMX20_02761 0.22 -1.6 2,918,093 + MPMX20_02761 0.26 -1.4 2,918,159 + MPMX20_02761 0.31 -0.5 2,918,174 + MPMX20_02761 0.32 -1.3 2,918,237 + MPMX20_02761 0.37 -0.3 2,918,295 + MPMX20_02761 0.41 -0.3 2,918,339 - MPMX20_02761 0.44 +0.6 2,918,372 - MPMX20_02761 0.47 -0.5 2,918,428 + MPMX20_02761 0.51 -0.6 2,918,431 - MPMX20_02761 0.51 +0.2
Or see this region's nucleotide sequence