Experiment: AMD2036 coinoculated with AMD2587 on RCH2 to a starting OD of 0.01
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00188 and MPMX20_00189 are separated by 91 nucleotides MPMX20_00189 and MPMX20_00190 are separated by 620 nucleotides MPMX20_00190 and MPMX20_00191 are separated by 161 nucleotides
MPMX20_00188: MPMX20_00188 - Kdo(2)-lipid A phosphoethanolamine 7''-transferase, at 195,895 to 197,589
_00188
MPMX20_00189: MPMX20_00189 - tRNA-Pro, at 197,681 to 197,757
_00189
MPMX20_00190: MPMX20_00190 - Periplasmic dipeptide transport protein, at 198,378 to 199,985
_00190
MPMX20_00191: MPMX20_00191 - Dipeptide transport system permease protein DppB, at 200,147 to 201,166
_00191
Position (kb) 198
199
200 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 197.421 kb on + strand at 197.422 kb on + strand at 197.423 kb on + strand at 197.471 kb on + strand at 197.476 kb on - strand at 197.479 kb on - strand at 197.481 kb on + strand at 197.491 kb on + strand at 197.499 kb on - strand at 197.505 kb on + strand at 197.505 kb on + strand at 197.505 kb on + strand at 197.506 kb on + strand at 197.513 kb on - strand at 197.514 kb on - strand at 197.514 kb on - strand at 197.514 kb on - strand at 197.514 kb on - strand at 197.579 kb on - strand at 197.579 kb on - strand at 197.597 kb on + strand at 197.652 kb on + strand at 197.660 kb on - strand at 197.843 kb on + strand at 197.929 kb on + strand at 197.984 kb on + strand at 197.989 kb on + strand at 198.028 kb on + strand at 198.150 kb on - strand at 198.154 kb on + strand at 198.154 kb on + strand at 198.154 kb on + strand at 198.154 kb on + strand at 198.154 kb on + strand at 198.154 kb on + strand at 198.162 kb on - strand at 198.173 kb on + strand at 198.181 kb on - strand at 198.254 kb on - strand at 198.296 kb on + strand at 198.301 kb on + strand at 198.337 kb on + strand at 198.345 kb on - strand at 198.345 kb on - strand at 198.345 kb on - strand at 198.353 kb on - strand at 198.363 kb on + strand at 198.397 kb on + strand at 198.412 kb on + strand at 198.415 kb on - strand at 198.415 kb on - strand at 198.420 kb on - strand at 198.425 kb on - strand at 198.426 kb on - strand at 198.426 kb on - strand at 198.440 kb on - strand at 198.478 kb on + strand at 198.486 kb on - strand at 198.512 kb on + strand at 198.515 kb on + strand at 198.523 kb on - strand at 198.544 kb on + strand, within MPMX20_00190 at 198.544 kb on + strand, within MPMX20_00190 at 198.552 kb on - strand, within MPMX20_00190 at 198.552 kb on - strand, within MPMX20_00190 at 198.555 kb on + strand, within MPMX20_00190 at 198.563 kb on - strand, within MPMX20_00190 at 198.638 kb on + strand, within MPMX20_00190 at 198.644 kb on + strand, within MPMX20_00190 at 198.644 kb on + strand, within MPMX20_00190 at 198.644 kb on + strand, within MPMX20_00190 at 198.644 kb on + strand, within MPMX20_00190 at 198.644 kb on + strand, within MPMX20_00190 at 198.644 kb on + strand, within MPMX20_00190 at 198.646 kb on - strand, within MPMX20_00190 at 198.652 kb on + strand, within MPMX20_00190 at 198.652 kb on - strand, within MPMX20_00190 at 198.652 kb on - strand, within MPMX20_00190 at 198.652 kb on - strand, within MPMX20_00190 at 198.652 kb on - strand, within MPMX20_00190 at 198.652 kb on - strand, within MPMX20_00190 at 198.652 kb on - strand, within MPMX20_00190 at 198.652 kb on - strand, within MPMX20_00190 at 198.652 kb on - strand, within MPMX20_00190 at 198.653 kb on - strand, within MPMX20_00190 at 198.660 kb on - strand, within MPMX20_00190 at 198.662 kb on + strand, within MPMX20_00190 at 198.734 kb on + strand, within MPMX20_00190 at 198.793 kb on + strand, within MPMX20_00190 at 198.801 kb on - strand, within MPMX20_00190 at 198.816 kb on + strand, within MPMX20_00190 at 198.816 kb on + strand, within MPMX20_00190 at 198.899 kb on - strand, within MPMX20_00190 at 198.906 kb on + strand, within MPMX20_00190 at 198.933 kb on - strand, within MPMX20_00190 at 198.982 kb on + strand, within MPMX20_00190 at 199.003 kb on + strand, within MPMX20_00190 at 199.003 kb on + strand, within MPMX20_00190 at 199.038 kb on - strand, within MPMX20_00190 at 199.060 kb on - strand, within MPMX20_00190 at 199.111 kb on - strand, within MPMX20_00190 at 199.117 kb on + strand, within MPMX20_00190 at 199.174 kb on - strand, within MPMX20_00190 at 199.242 kb on + strand, within MPMX20_00190 at 199.318 kb on + strand, within MPMX20_00190 at 199.319 kb on - strand, within MPMX20_00190 at 199.324 kb on - strand, within MPMX20_00190 at 199.324 kb on - strand, within MPMX20_00190 at 199.428 kb on - strand, within MPMX20_00190 at 199.460 kb on - strand, within MPMX20_00190 at 199.471 kb on + strand, within MPMX20_00190 at 199.471 kb on - strand, within MPMX20_00190 at 199.476 kb on + strand, within MPMX20_00190 at 199.476 kb on + strand, within MPMX20_00190 at 199.484 kb on - strand, within MPMX20_00190 at 199.510 kb on - strand, within MPMX20_00190 at 199.510 kb on - strand, within MPMX20_00190 at 199.513 kb on + strand, within MPMX20_00190 at 199.521 kb on - strand, within MPMX20_00190 at 199.521 kb on - strand, within MPMX20_00190 at 199.534 kb on + strand, within MPMX20_00190 at 199.576 kb on - strand, within MPMX20_00190 at 199.584 kb on - strand, within MPMX20_00190 at 199.685 kb on + strand, within MPMX20_00190 at 199.698 kb on + strand, within MPMX20_00190 at 199.713 kb on + strand, within MPMX20_00190 at 199.718 kb on + strand, within MPMX20_00190 at 199.726 kb on - strand, within MPMX20_00190 at 199.726 kb on - strand, within MPMX20_00190 at 199.726 kb on - strand, within MPMX20_00190 at 199.790 kb on - strand, within MPMX20_00190 at 199.852 kb on - strand at 199.852 kb on - strand at 199.880 kb on + strand at 199.950 kb on + strand at 199.994 kb on + strand at 200.007 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.019 kb on + strand at 200.027 kb on - strand at 200.027 kb on - strand at 200.027 kb on - strand at 200.027 kb on - strand at 200.027 kb on - strand at 200.027 kb on - strand at 200.027 kb on - strand at 200.027 kb on - strand at 200.052 kb on + strand at 200.052 kb on + strand at 200.052 kb on + strand at 200.052 kb on + strand at 200.052 kb on + strand at 200.052 kb on + strand at 200.052 kb on + strand at 200.060 kb on - strand at 200.060 kb on - strand at 200.060 kb on - strand at 200.060 kb on - strand at 200.064 kb on + strand at 200.070 kb on - strand at 200.097 kb on + strand at 200.105 kb on - strand at 200.121 kb on - strand at 200.169 kb on + strand at 200.177 kb on - strand at 200.299 kb on + strand, within MPMX20_00191 at 200.310 kb on + strand, within MPMX20_00191 at 200.315 kb on + strand, within MPMX20_00191 at 200.318 kb on - strand, within MPMX20_00191 at 200.328 kb on - strand, within MPMX20_00191 at 200.418 kb on + strand, within MPMX20_00191 at 200.485 kb on + strand, within MPMX20_00191 at 200.493 kb on - strand, within MPMX20_00191 at 200.508 kb on - strand, within MPMX20_00191 at 200.563 kb on + strand, within MPMX20_00191 at 200.610 kb on - strand, within MPMX20_00191 at 200.631 kb on - strand, within MPMX20_00191 at 200.725 kb on - strand, within MPMX20_00191 at 200.731 kb on + strand, within MPMX20_00191 at 200.757 kb on + strand, within MPMX20_00191 at 200.784 kb on + strand, within MPMX20_00191 at 200.818 kb on - strand, within MPMX20_00191 at 200.831 kb on - strand, within MPMX20_00191 at 200.869 kb on + strand, within MPMX20_00191 at 200.876 kb on + strand, within MPMX20_00191 at 200.884 kb on - strand, within MPMX20_00191 at 200.901 kb on - strand, within MPMX20_00191 at 200.914 kb on + strand, within MPMX20_00191 at 200.914 kb on + strand, within MPMX20_00191 at 200.914 kb on + strand, within MPMX20_00191 at 200.919 kb on + strand, within MPMX20_00191 at 200.922 kb on - strand, within MPMX20_00191 at 200.956 kb on + strand, within MPMX20_00191
Per-strain Table
Position Strand Gene LocusTag Fraction AMD2036 coinoculated with AMD2587 on RCH2 to a starting OD of 0.01 remove 197,421 + -1.1 197,422 + +2.1 197,423 + +0.5 197,471 + -0.2 197,476 - -0.4 197,479 - +0.6 197,481 + +2.0 197,491 + -0.6 197,499 - -0.2 197,505 + +0.7 197,505 + +1.6 197,505 + -1.1 197,506 + +0.6 197,513 - +0.0 197,514 - -0.4 197,514 - +0.8 197,514 - +0.1 197,514 - -1.5 197,579 - +0.6 197,579 - -0.7 197,597 + +1.0 197,652 + +0.5 197,660 - -1.2 197,843 + -2.0 197,929 + +0.1 197,984 + +0.1 197,989 + -0.4 198,028 + +0.9 198,150 - +0.5 198,154 + -1.4 198,154 + -0.3 198,154 + -0.2 198,154 + +0.3 198,154 + -1.8 198,154 + +0.7 198,162 - +1.0 198,173 + +1.0 198,181 - +0.2 198,254 - +0.1 198,296 + -0.9 198,301 + +0.9 198,337 + -1.7 198,345 - -0.0 198,345 - -0.4 198,345 - -2.2 198,353 - +0.6 198,363 + +0.4 198,397 + -1.4 198,412 + +0.1 198,415 - +1.2 198,415 - +1.6 198,420 - -0.7 198,425 - -3.0 198,426 - -0.6 198,426 - -0.7 198,440 - -0.2 198,478 + -0.8 198,486 - +1.3 198,512 + +1.2 198,515 + +0.5 198,523 - -2.7 198,544 + MPMX20_00190 0.10 -1.1 198,544 + MPMX20_00190 0.10 +0.9 198,552 - MPMX20_00190 0.11 +1.1 198,552 - MPMX20_00190 0.11 -0.0 198,555 + MPMX20_00190 0.11 +0.9 198,563 - MPMX20_00190 0.12 -1.1 198,638 + MPMX20_00190 0.16 +0.1 198,644 + MPMX20_00190 0.17 -2.3 198,644 + MPMX20_00190 0.17 +0.5 198,644 + MPMX20_00190 0.17 -1.1 198,644 + MPMX20_00190 0.17 +1.0 198,644 + MPMX20_00190 0.17 +0.5 198,644 + MPMX20_00190 0.17 +0.6 198,646 - MPMX20_00190 0.17 +1.9 198,652 + MPMX20_00190 0.17 +3.0 198,652 - MPMX20_00190 0.17 +0.3 198,652 - MPMX20_00190 0.17 +1.6 198,652 - MPMX20_00190 0.17 +1.4 198,652 - MPMX20_00190 0.17 -1.2 198,652 - MPMX20_00190 0.17 -0.8 198,652 - MPMX20_00190 0.17 +2.2 198,652 - MPMX20_00190 0.17 -0.8 198,652 - MPMX20_00190 0.17 -1.0 198,653 - MPMX20_00190 0.17 -0.4 198,660 - MPMX20_00190 0.18 +0.1 198,662 + MPMX20_00190 0.18 -0.6 198,734 + MPMX20_00190 0.22 +1.0 198,793 + MPMX20_00190 0.26 -0.7 198,801 - MPMX20_00190 0.26 -1.0 198,816 + MPMX20_00190 0.27 +0.8 198,816 + MPMX20_00190 0.27 -1.5 198,899 - MPMX20_00190 0.32 +0.8 198,906 + MPMX20_00190 0.33 -0.2 198,933 - MPMX20_00190 0.35 -1.0 198,982 + MPMX20_00190 0.38 +0.5 199,003 + MPMX20_00190 0.39 -2.1 199,003 + MPMX20_00190 0.39 -0.5 199,038 - MPMX20_00190 0.41 -0.0 199,060 - MPMX20_00190 0.42 -0.6 199,111 - MPMX20_00190 0.46 +1.1 199,117 + MPMX20_00190 0.46 -0.0 199,174 - MPMX20_00190 0.50 -0.7 199,242 + MPMX20_00190 0.54 -0.9 199,318 + MPMX20_00190 0.58 -0.3 199,319 - MPMX20_00190 0.59 -0.0 199,324 - MPMX20_00190 0.59 +0.3 199,324 - MPMX20_00190 0.59 +0.0 199,428 - MPMX20_00190 0.65 -0.8 199,460 - MPMX20_00190 0.67 -1.0 199,471 + MPMX20_00190 0.68 -2.6 199,471 - MPMX20_00190 0.68 -1.1 199,476 + MPMX20_00190 0.68 -2.8 199,476 + MPMX20_00190 0.68 +1.2 199,484 - MPMX20_00190 0.69 -0.5 199,510 - MPMX20_00190 0.70 +0.7 199,510 - MPMX20_00190 0.70 -1.6 199,513 + MPMX20_00190 0.71 +1.8 199,521 - MPMX20_00190 0.71 -0.6 199,521 - MPMX20_00190 0.71 -1.8 199,534 + MPMX20_00190 0.72 +0.6 199,576 - MPMX20_00190 0.75 -1.8 199,584 - MPMX20_00190 0.75 -0.5 199,685 + MPMX20_00190 0.81 -0.6 199,698 + MPMX20_00190 0.82 +0.5 199,713 + MPMX20_00190 0.83 -2.7 199,718 + MPMX20_00190 0.83 +0.8 199,726 - MPMX20_00190 0.84 -0.6 199,726 - MPMX20_00190 0.84 +0.1 199,726 - MPMX20_00190 0.84 -0.6 199,790 - MPMX20_00190 0.88 +0.6 199,852 - -0.5 199,852 - -2.1 199,880 + -0.0 199,950 + -0.4 199,994 + +0.6 200,007 + +0.5 200,019 + +0.8 200,019 + -1.0 200,019 + +0.2 200,019 + +0.2 200,019 + +0.1 200,019 + +0.9 200,019 + +0.5 200,019 + -0.4 200,019 + -2.3 200,019 + -1.7 200,019 + -0.7 200,019 + -0.6 200,019 + -0.4 200,019 + -0.2 200,019 + -0.8 200,019 + +0.5 200,019 + +0.2 200,027 - -1.8 200,027 - +0.8 200,027 - -0.4 200,027 - -0.3 200,027 - -0.1 200,027 - -1.4 200,027 - -1.2 200,027 - -0.2 200,052 + +0.2 200,052 + -1.5 200,052 + +0.2 200,052 + -0.1 200,052 + -0.1 200,052 + -1.2 200,052 + +0.0 200,060 - -2.1 200,060 - +1.1 200,060 - -0.1 200,060 - +0.1 200,064 + +0.5 200,070 - -0.2 200,097 + +0.6 200,105 - +0.8 200,121 - +1.4 200,169 + +0.9 200,177 - -0.6 200,299 + MPMX20_00191 0.15 -1.1 200,310 + MPMX20_00191 0.16 -1.4 200,315 + MPMX20_00191 0.16 +0.5 200,318 - MPMX20_00191 0.17 -0.9 200,328 - MPMX20_00191 0.18 -1.3 200,418 + MPMX20_00191 0.27 -0.6 200,485 + MPMX20_00191 0.33 +0.2 200,493 - MPMX20_00191 0.34 -1.3 200,508 - MPMX20_00191 0.35 -1.0 200,563 + MPMX20_00191 0.41 +0.8 200,610 - MPMX20_00191 0.45 -1.2 200,631 - MPMX20_00191 0.47 -1.0 200,725 - MPMX20_00191 0.57 +0.5 200,731 + MPMX20_00191 0.57 -0.6 200,757 + MPMX20_00191 0.60 +0.1 200,784 + MPMX20_00191 0.62 -1.8 200,818 - MPMX20_00191 0.66 -2.0 200,831 - MPMX20_00191 0.67 -0.0 200,869 + MPMX20_00191 0.71 +1.0 200,876 + MPMX20_00191 0.71 +1.0 200,884 - MPMX20_00191 0.72 +0.6 200,901 - MPMX20_00191 0.74 +2.2 200,914 + MPMX20_00191 0.75 +0.7 200,914 + MPMX20_00191 0.75 -0.1 200,914 + MPMX20_00191 0.75 -1.3 200,919 + MPMX20_00191 0.76 -2.6 200,922 - MPMX20_00191 0.76 -1.4 200,956 + MPMX20_00191 0.79 +0.9
Or see this region's nucleotide sequence