Experiment: AMD2036 coinoculated with AMD2587 on TSA to a starting OD of 0.001
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00897 and MPMX20_00898 are separated by 32 nucleotides MPMX20_00898 and MPMX20_00899 are separated by 0 nucleotides MPMX20_00899 and MPMX20_00900 are separated by 55 nucleotides MPMX20_00900 and MPMX20_00901 are separated by 132 nucleotides
MPMX20_00897: MPMX20_00897 - Hydroxyacylglutathione hydrolase GloB, at 945,959 to 946,717
_00897
MPMX20_00898: MPMX20_00898 - hypothetical protein, at 946,750 to 947,466
_00898
MPMX20_00899: MPMX20_00899 - Ribonuclease HI, at 947,467 to 947,934
_00899
MPMX20_00900: MPMX20_00900 - DNA polymerase III subunit epsilon, at 947,990 to 948,730
_00900
MPMX20_00901: MPMX20_00901 - tRNA-Asp, at 948,863 to 948,939
_00901
Position (kb) 947
948 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 946.467 kb on + strand, within MPMX20_00897 at 946.544 kb on - strand, within MPMX20_00897 at 946.567 kb on + strand, within MPMX20_00897 at 946.570 kb on - strand, within MPMX20_00897 at 946.583 kb on - strand, within MPMX20_00897 at 946.693 kb on - strand at 946.694 kb on + strand at 946.711 kb on + strand at 946.749 kb on + strand at 946.786 kb on + strand at 946.806 kb on + strand at 946.812 kb on + strand at 946.814 kb on - strand at 946.814 kb on - strand at 946.814 kb on - strand at 946.814 kb on - strand at 946.814 kb on - strand at 946.833 kb on + strand, within MPMX20_00898 at 946.837 kb on + strand, within MPMX20_00898 at 946.884 kb on + strand, within MPMX20_00898 at 946.928 kb on + strand, within MPMX20_00898 at 946.936 kb on - strand, within MPMX20_00898 at 946.944 kb on + strand, within MPMX20_00898 at 946.947 kb on - strand, within MPMX20_00898 at 946.951 kb on - strand, within MPMX20_00898 at 946.972 kb on + strand, within MPMX20_00898 at 946.972 kb on + strand, within MPMX20_00898 at 947.143 kb on + strand, within MPMX20_00898 at 947.157 kb on + strand, within MPMX20_00898 at 947.178 kb on + strand, within MPMX20_00898 at 947.208 kb on + strand, within MPMX20_00898 at 947.216 kb on - strand, within MPMX20_00898 at 947.249 kb on + strand, within MPMX20_00898 at 947.311 kb on + strand, within MPMX20_00898 at 947.311 kb on + strand, within MPMX20_00898 at 947.314 kb on - strand, within MPMX20_00898 at 947.342 kb on + strand, within MPMX20_00898 at 947.349 kb on + strand, within MPMX20_00898 at 947.491 kb on + strand at 947.504 kb on + strand at 947.575 kb on - strand, within MPMX20_00899 at 947.576 kb on - strand, within MPMX20_00899 at 947.707 kb on - strand, within MPMX20_00899 at 947.785 kb on + strand, within MPMX20_00899 at 947.880 kb on + strand, within MPMX20_00899 at 947.903 kb on - strand at 947.996 kb on + strand at 947.996 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction AMD2036 coinoculated with AMD2587 on TSA to a starting OD of 0.001 remove 946,467 + MPMX20_00897 0.67 -0.5 946,544 - MPMX20_00897 0.77 -2.8 946,567 + MPMX20_00897 0.80 -1.0 946,570 - MPMX20_00897 0.81 -1.4 946,583 - MPMX20_00897 0.82 +2.2 946,693 - +1.0 946,694 + -0.2 946,711 + +0.9 946,749 + +0.7 946,786 + +0.2 946,806 + +2.4 946,812 + -2.4 946,814 - +1.3 946,814 - +1.8 946,814 - +1.5 946,814 - +1.2 946,814 - +2.6 946,833 + MPMX20_00898 0.12 -1.7 946,837 + MPMX20_00898 0.12 +2.9 946,884 + MPMX20_00898 0.19 +0.3 946,928 + MPMX20_00898 0.25 +1.2 946,936 - MPMX20_00898 0.26 -1.9 946,944 + MPMX20_00898 0.27 -1.9 946,947 - MPMX20_00898 0.27 +0.6 946,951 - MPMX20_00898 0.28 -3.1 946,972 + MPMX20_00898 0.31 -0.4 946,972 + MPMX20_00898 0.31 -3.2 947,143 + MPMX20_00898 0.55 +1.2 947,157 + MPMX20_00898 0.57 -0.9 947,178 + MPMX20_00898 0.60 +1.7 947,208 + MPMX20_00898 0.64 +1.7 947,216 - MPMX20_00898 0.65 -2.0 947,249 + MPMX20_00898 0.70 +0.5 947,311 + MPMX20_00898 0.78 -3.7 947,311 + MPMX20_00898 0.78 -1.6 947,314 - MPMX20_00898 0.79 -0.9 947,342 + MPMX20_00898 0.83 +0.9 947,349 + MPMX20_00898 0.84 -1.1 947,491 + -3.2 947,504 + +3.0 947,575 - MPMX20_00899 0.23 +0.5 947,576 - MPMX20_00899 0.23 -1.5 947,707 - MPMX20_00899 0.51 -0.8 947,785 + MPMX20_00899 0.68 -1.0 947,880 + MPMX20_00899 0.88 -0.7 947,903 - -1.0 947,996 + +0.3 947,996 + +2.9
Or see this region's nucleotide sequence