Experiment: AMD2036 coinoculated with AMD2587 on TSA to a starting OD of 0.001
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00665 and MPMX20_00666 are separated by 40 nucleotides MPMX20_00666 and MPMX20_00667 are separated by 32 nucleotides MPMX20_00667 and MPMX20_00668 are separated by 28 nucleotides MPMX20_00668 and MPMX20_00669 are separated by 173 nucleotides MPMX20_00669 and MPMX20_00670 are separated by 102 nucleotides MPMX20_00670 and MPMX20_00671 overlap by 32 nucleotides MPMX20_00671 and MPMX20_00672 are separated by 19 nucleotides
MPMX20_00665: MPMX20_00665 - hypothetical protein, at 687,747 to 687,977
_00665
MPMX20_00666: MPMX20_00666 - tRNA-Leu, at 688,018 to 688,104
_00666
MPMX20_00667: MPMX20_00667 - tRNA-Leu, at 688,137 to 688,223
_00667
MPMX20_00668: MPMX20_00668 - tRNA-Leu, at 688,252 to 688,338
_00668
MPMX20_00669: MPMX20_00669 - Ribosomal RNA small subunit methyltransferase C, at 688,512 to 689,540
_00669
MPMX20_00670: MPMX20_00670 - DNA polymerase III subunit psi, at 689,643 to 690,056
_00670
MPMX20_00671: MPMX20_00671 - [Ribosomal protein S18]-alanine N-acetyltransferase, at 690,025 to 690,468
_00671
MPMX20_00672: MPMX20_00672 - Pyrimidine 5'-nucleotidase YjjG, at 690,488 to 691,165
_00672
Position (kb) 688
689
690 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 687.601 kb on + strand at 687.603 kb on - strand at 687.606 kb on - strand at 687.608 kb on - strand at 687.609 kb on - strand at 687.609 kb on - strand at 687.609 kb on - strand at 687.614 kb on - strand at 687.616 kb on - strand at 687.624 kb on + strand at 687.625 kb on - strand at 687.631 kb on - strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.653 kb on + strand at 687.659 kb on + strand at 687.660 kb on - strand at 687.660 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.661 kb on - strand at 687.747 kb on + strand at 687.747 kb on + strand at 687.747 kb on + strand at 687.747 kb on + strand at 687.752 kb on + strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.755 kb on - strand at 687.757 kb on - strand at 687.761 kb on + strand at 687.764 kb on + strand at 687.764 kb on + strand at 687.769 kb on - strand at 687.772 kb on - strand, within MPMX20_00665 at 687.801 kb on - strand, within MPMX20_00665 at 687.808 kb on + strand, within MPMX20_00665 at 687.812 kb on + strand, within MPMX20_00665 at 687.859 kb on - strand, within MPMX20_00665 at 687.874 kb on + strand, within MPMX20_00665 at 687.874 kb on + strand, within MPMX20_00665 at 687.874 kb on + strand, within MPMX20_00665 at 687.879 kb on + strand, within MPMX20_00665 at 687.882 kb on - strand, within MPMX20_00665 at 687.882 kb on - strand, within MPMX20_00665 at 687.882 kb on - strand, within MPMX20_00665 at 687.882 kb on - strand, within MPMX20_00665 at 687.889 kb on + strand, within MPMX20_00665 at 687.889 kb on + strand, within MPMX20_00665 at 687.928 kb on + strand, within MPMX20_00665 at 687.933 kb on + strand, within MPMX20_00665 at 687.933 kb on + strand, within MPMX20_00665 at 687.933 kb on + strand, within MPMX20_00665 at 687.933 kb on + strand, within MPMX20_00665 at 687.936 kb on - strand, within MPMX20_00665 at 687.936 kb on - strand, within MPMX20_00665 at 687.941 kb on - strand, within MPMX20_00665 at 687.951 kb on - strand, within MPMX20_00665 at 688.041 kb on - strand, within MPMX20_00666 at 688.048 kb on - strand, within MPMX20_00666 at 688.269 kb on - strand, within MPMX20_00668 at 688.381 kb on + strand at 688.389 kb on - strand at 688.426 kb on + strand at 688.434 kb on - strand at 688.496 kb on - strand at 688.515 kb on - strand at 688.532 kb on + strand at 688.540 kb on - strand at 688.549 kb on + strand at 688.558 kb on - strand at 688.558 kb on - strand at 688.595 kb on - strand at 688.659 kb on + strand, within MPMX20_00669 at 688.659 kb on + strand, within MPMX20_00669 at 688.713 kb on + strand, within MPMX20_00669 at 688.831 kb on - strand, within MPMX20_00669 at 688.868 kb on + strand, within MPMX20_00669 at 688.876 kb on - strand, within MPMX20_00669 at 688.876 kb on - strand, within MPMX20_00669 at 688.924 kb on - strand, within MPMX20_00669 at 688.935 kb on + strand, within MPMX20_00669 at 688.935 kb on + strand, within MPMX20_00669 at 688.994 kb on + strand, within MPMX20_00669 at 688.994 kb on + strand, within MPMX20_00669 at 688.994 kb on + strand, within MPMX20_00669 at 689.002 kb on - strand, within MPMX20_00669 at 689.003 kb on - strand, within MPMX20_00669 at 689.044 kb on + strand, within MPMX20_00669 at 689.051 kb on + strand, within MPMX20_00669 at 689.059 kb on - strand, within MPMX20_00669 at 689.120 kb on - strand, within MPMX20_00669 at 689.125 kb on - strand, within MPMX20_00669 at 689.161 kb on - strand, within MPMX20_00669 at 689.177 kb on - strand, within MPMX20_00669 at 689.199 kb on + strand, within MPMX20_00669 at 689.294 kb on + strand, within MPMX20_00669 at 689.325 kb on + strand, within MPMX20_00669 at 689.328 kb on - strand, within MPMX20_00669 at 689.336 kb on - strand, within MPMX20_00669 at 689.381 kb on - strand, within MPMX20_00669 at 689.400 kb on - strand, within MPMX20_00669 at 689.402 kb on - strand, within MPMX20_00669 at 689.406 kb on - strand, within MPMX20_00669 at 689.415 kb on + strand, within MPMX20_00669 at 689.423 kb on - strand, within MPMX20_00669 at 689.425 kb on - strand, within MPMX20_00669 at 689.427 kb on + strand, within MPMX20_00669 at 689.427 kb on + strand, within MPMX20_00669 at 689.435 kb on - strand, within MPMX20_00669 at 689.448 kb on + strand at 689.461 kb on - strand at 689.471 kb on + strand at 689.497 kb on + strand at 689.505 kb on + strand at 689.518 kb on - strand at 689.573 kb on + strand at 689.592 kb on + strand at 689.620 kb on + strand at 689.628 kb on - strand at 689.628 kb on - strand at 689.829 kb on - strand, within MPMX20_00670 at 690.050 kb on + strand at 690.058 kb on - strand at 690.058 kb on - strand at 690.074 kb on + strand, within MPMX20_00671 at 690.198 kb on + strand, within MPMX20_00671 at 690.222 kb on + strand, within MPMX20_00671 at 690.222 kb on + strand, within MPMX20_00671 at 690.222 kb on + strand, within MPMX20_00671 at 690.222 kb on + strand, within MPMX20_00671 at 690.230 kb on - strand, within MPMX20_00671 at 690.230 kb on - strand, within MPMX20_00671 at 690.247 kb on + strand, within MPMX20_00671 at 690.274 kb on - strand, within MPMX20_00671 at 690.334 kb on - strand, within MPMX20_00671 at 690.334 kb on - strand, within MPMX20_00671 at 690.334 kb on - strand, within MPMX20_00671 at 690.334 kb on - strand, within MPMX20_00671 at 690.334 kb on - strand, within MPMX20_00671 at 690.372 kb on - strand, within MPMX20_00671 at 690.382 kb on - strand, within MPMX20_00671 at 690.419 kb on + strand, within MPMX20_00671 at 690.419 kb on + strand, within MPMX20_00671 at 690.427 kb on - strand at 690.444 kb on + strand at 690.529 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction AMD2036 coinoculated with AMD2587 on TSA to a starting OD of 0.001 remove 687,601 + +0.5 687,603 - -4.0 687,606 - -1.1 687,608 - +1.5 687,609 - -2.9 687,609 - +2.1 687,609 - +1.4 687,614 - -2.9 687,616 - +0.8 687,624 + -2.2 687,625 - -1.2 687,631 - -1.2 687,653 + +1.3 687,653 + +1.8 687,653 + +0.2 687,653 + -0.9 687,653 + +1.3 687,653 + +2.9 687,653 + -2.4 687,653 + -1.4 687,653 + -1.4 687,653 + +2.2 687,659 + -1.0 687,660 - +1.4 687,660 - +2.0 687,661 - +1.5 687,661 - +1.9 687,661 - -3.3 687,661 - +0.2 687,661 - -0.8 687,661 - +1.9 687,661 - +0.0 687,661 - +1.5 687,661 - -1.0 687,661 - +2.7 687,661 - -0.8 687,661 - -0.7 687,747 + -2.0 687,747 + +0.4 687,747 + -1.3 687,747 + -1.8 687,752 + -2.3 687,755 - -1.4 687,755 - +2.0 687,755 - -3.1 687,755 - +1.4 687,755 - +0.1 687,755 - +1.2 687,755 - -3.3 687,755 - +0.3 687,755 - -2.9 687,755 - -2.7 687,757 - -2.9 687,761 + +1.6 687,764 + +1.7 687,764 + +1.7 687,769 - -1.8 687,772 - MPMX20_00665 0.11 -3.2 687,801 - MPMX20_00665 0.23 +2.6 687,808 + MPMX20_00665 0.26 +1.7 687,812 + MPMX20_00665 0.28 -1.1 687,859 - MPMX20_00665 0.48 -2.7 687,874 + MPMX20_00665 0.55 +0.5 687,874 + MPMX20_00665 0.55 +3.4 687,874 + MPMX20_00665 0.55 +1.6 687,879 + MPMX20_00665 0.57 -3.4 687,882 - MPMX20_00665 0.58 -3.1 687,882 - MPMX20_00665 0.58 -0.0 687,882 - MPMX20_00665 0.58 +0.3 687,882 - MPMX20_00665 0.58 -0.3 687,889 + MPMX20_00665 0.61 -1.1 687,889 + MPMX20_00665 0.61 -0.7 687,928 + MPMX20_00665 0.78 +1.6 687,933 + MPMX20_00665 0.81 +4.0 687,933 + MPMX20_00665 0.81 -3.5 687,933 + MPMX20_00665 0.81 +2.0 687,933 + MPMX20_00665 0.81 +1.2 687,936 - MPMX20_00665 0.82 +1.7 687,936 - MPMX20_00665 0.82 -2.7 687,941 - MPMX20_00665 0.84 -1.1 687,951 - MPMX20_00665 0.88 -4.3 688,041 - MPMX20_00666 0.26 -0.6 688,048 - MPMX20_00666 0.34 +1.0 688,269 - MPMX20_00668 0.20 -1.1 688,381 + -1.1 688,389 - -2.7 688,426 + -0.4 688,434 - +0.8 688,496 - -2.6 688,515 - +1.4 688,532 + -2.5 688,540 - -0.3 688,549 + -0.5 688,558 - -2.6 688,558 - -3.2 688,595 - +1.9 688,659 + MPMX20_00669 0.14 +0.0 688,659 + MPMX20_00669 0.14 -2.4 688,713 + MPMX20_00669 0.20 -1.1 688,831 - MPMX20_00669 0.31 +2.0 688,868 + MPMX20_00669 0.35 -1.8 688,876 - MPMX20_00669 0.35 +0.2 688,876 - MPMX20_00669 0.35 +1.6 688,924 - MPMX20_00669 0.40 +0.2 688,935 + MPMX20_00669 0.41 -2.0 688,935 + MPMX20_00669 0.41 +0.8 688,994 + MPMX20_00669 0.47 -0.3 688,994 + MPMX20_00669 0.47 +0.3 688,994 + MPMX20_00669 0.47 -2.0 689,002 - MPMX20_00669 0.48 +1.4 689,003 - MPMX20_00669 0.48 +1.5 689,044 + MPMX20_00669 0.52 -1.7 689,051 + MPMX20_00669 0.52 -0.4 689,059 - MPMX20_00669 0.53 -1.0 689,120 - MPMX20_00669 0.59 -2.9 689,125 - MPMX20_00669 0.60 -1.1 689,161 - MPMX20_00669 0.63 +3.4 689,177 - MPMX20_00669 0.65 -0.2 689,199 + MPMX20_00669 0.67 +0.4 689,294 + MPMX20_00669 0.76 +2.1 689,325 + MPMX20_00669 0.79 +2.2 689,328 - MPMX20_00669 0.79 -2.5 689,336 - MPMX20_00669 0.80 -2.1 689,381 - MPMX20_00669 0.84 -2.6 689,400 - MPMX20_00669 0.86 -0.7 689,402 - MPMX20_00669 0.86 -1.4 689,406 - MPMX20_00669 0.87 +1.6 689,415 + MPMX20_00669 0.88 +1.4 689,423 - MPMX20_00669 0.89 +1.4 689,425 - MPMX20_00669 0.89 -3.5 689,427 + MPMX20_00669 0.89 +1.5 689,427 + MPMX20_00669 0.89 +2.0 689,435 - MPMX20_00669 0.90 +2.4 689,448 + +1.0 689,461 - +1.7 689,471 + +1.2 689,497 + +0.7 689,505 + -0.9 689,518 - +0.2 689,573 + -0.7 689,592 + +0.5 689,620 + -0.3 689,628 - -2.8 689,628 - +1.7 689,829 - MPMX20_00670 0.45 -0.7 690,050 + -0.4 690,058 - -2.7 690,058 - +1.4 690,074 + MPMX20_00671 0.11 +1.4 690,198 + MPMX20_00671 0.39 +0.7 690,222 + MPMX20_00671 0.44 -1.1 690,222 + MPMX20_00671 0.44 -3.0 690,222 + MPMX20_00671 0.44 -1.8 690,222 + MPMX20_00671 0.44 -0.6 690,230 - MPMX20_00671 0.46 -0.7 690,230 - MPMX20_00671 0.46 -0.9 690,247 + MPMX20_00671 0.50 -3.1 690,274 - MPMX20_00671 0.56 -1.1 690,334 - MPMX20_00671 0.70 -0.3 690,334 - MPMX20_00671 0.70 +1.0 690,334 - MPMX20_00671 0.70 +2.7 690,334 - MPMX20_00671 0.70 +1.6 690,334 - MPMX20_00671 0.70 -2.3 690,372 - MPMX20_00671 0.78 -2.4 690,382 - MPMX20_00671 0.80 -3.4 690,419 + MPMX20_00671 0.89 -2.0 690,419 + MPMX20_00671 0.89 +1.1 690,427 - -0.9 690,444 + +0.0 690,529 + +2.4
Or see this region's nucleotide sequence