Experiment: AMD2036 coinoculated with AMD2587 on LB to a starting OD of 0.01
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_02764 and MPMX20_02765 overlap by 4 nucleotides MPMX20_02765 and MPMX20_02766 are separated by 39 nucleotides MPMX20_02766 and MPMX20_02767 are separated by 8 nucleotides MPMX20_02767 and MPMX20_02768 are separated by 80 nucleotides
MPMX20_02764: MPMX20_02764 - Zinc import ATP-binding protein ZnuC, at 2,920,109 to 2,920,864
_02764
MPMX20_02765: MPMX20_02765 - High-affinity zinc uptake system membrane protein ZnuB, at 2,920,861 to 2,921,646
_02765
MPMX20_02766: MPMX20_02766 - Holliday junction ATP-dependent DNA helicase RuvB, at 2,921,686 to 2,922,696
_02766
MPMX20_02767: MPMX20_02767 - Holliday junction ATP-dependent DNA helicase RuvA, at 2,922,705 to 2,923,316
_02767
MPMX20_02768: MPMX20_02768 - Crossover junction endodeoxyribonuclease RuvC, at 2,923,397 to 2,923,918
_02768
Position (kb)
2921
2922
2923 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2920.687 kb on - strand, within MPMX20_02764 at 2920.707 kb on + strand, within MPMX20_02764 at 2920.724 kb on - strand, within MPMX20_02764 at 2920.778 kb on - strand, within MPMX20_02764 at 2920.895 kb on + strand at 2920.903 kb on - strand at 2920.903 kb on - strand at 2920.982 kb on + strand, within MPMX20_02765 at 2921.000 kb on + strand, within MPMX20_02765 at 2921.000 kb on + strand, within MPMX20_02765 at 2921.068 kb on + strand, within MPMX20_02765 at 2921.078 kb on - strand, within MPMX20_02765 at 2921.150 kb on + strand, within MPMX20_02765 at 2921.430 kb on + strand, within MPMX20_02765 at 2921.550 kb on - strand, within MPMX20_02765 at 2921.593 kb on - strand at 2921.595 kb on - strand at 2921.650 kb on + strand at 2921.663 kb on - strand at 2921.675 kb on + strand at 2921.778 kb on + strand at 2921.778 kb on + strand at 2921.893 kb on - strand, within MPMX20_02766 at 2921.903 kb on - strand, within MPMX20_02766 at 2922.050 kb on - strand, within MPMX20_02766 at 2922.194 kb on - strand, within MPMX20_02766 at 2922.335 kb on + strand, within MPMX20_02766 at 2922.355 kb on - strand, within MPMX20_02766 at 2922.389 kb on - strand, within MPMX20_02766 at 2922.389 kb on - strand, within MPMX20_02766 at 2922.389 kb on - strand, within MPMX20_02766 at 2922.391 kb on + strand, within MPMX20_02766 at 2922.440 kb on - strand, within MPMX20_02766 at 2922.458 kb on - strand, within MPMX20_02766 at 2922.491 kb on + strand, within MPMX20_02766 at 2922.626 kb on - strand at 2922.881 kb on + strand, within MPMX20_02767 at 2922.892 kb on + strand, within MPMX20_02767 at 2922.900 kb on - strand, within MPMX20_02767 at 2922.916 kb on + strand, within MPMX20_02767 at 2922.916 kb on + strand, within MPMX20_02767 at 2922.979 kb on + strand, within MPMX20_02767 at 2922.987 kb on - strand, within MPMX20_02767 at 2923.001 kb on - strand, within MPMX20_02767 at 2923.034 kb on - strand, within MPMX20_02767 at 2923.158 kb on - strand, within MPMX20_02767 at 2923.242 kb on + strand, within MPMX20_02767 at 2923.268 kb on - strand at 2923.331 kb on - strand at 2923.407 kb on - strand at 2923.447 kb on - strand at 2923.447 kb on - strand at 2923.471 kb on + strand, within MPMX20_02768 at 2923.479 kb on - strand, within MPMX20_02768 at 2923.526 kb on + strand, within MPMX20_02768 at 2923.526 kb on + strand, within MPMX20_02768 at 2923.532 kb on - strand, within MPMX20_02768
Per-strain Table
Position Strand Gene LocusTag Fraction AMD2036 coinoculated with AMD2587 on LB to a starting OD of 0.01 remove 2,920,687 - MPMX20_02764 0.76 +0.1 2,920,707 + MPMX20_02764 0.79 -0.1 2,920,724 - MPMX20_02764 0.81 -1.8 2,920,778 - MPMX20_02764 0.88 +0.7 2,920,895 + -0.2 2,920,903 - -0.5 2,920,903 - +0.4 2,920,982 + MPMX20_02765 0.15 +0.4 2,921,000 + MPMX20_02765 0.18 -1.1 2,921,000 + MPMX20_02765 0.18 -1.5 2,921,068 + MPMX20_02765 0.26 +2.7 2,921,078 - MPMX20_02765 0.28 -1.2 2,921,150 + MPMX20_02765 0.37 -0.7 2,921,430 + MPMX20_02765 0.72 -0.6 2,921,550 - MPMX20_02765 0.88 -0.9 2,921,593 - -1.5 2,921,595 - -0.6 2,921,650 + +0.7 2,921,663 - -0.1 2,921,675 + -0.4 2,921,778 + -1.4 2,921,778 + +1.1 2,921,893 - MPMX20_02766 0.20 -1.1 2,921,903 - MPMX20_02766 0.21 -2.6 2,922,050 - MPMX20_02766 0.36 -0.1 2,922,194 - MPMX20_02766 0.50 -0.3 2,922,335 + MPMX20_02766 0.64 +0.1 2,922,355 - MPMX20_02766 0.66 -0.3 2,922,389 - MPMX20_02766 0.70 -1.3 2,922,389 - MPMX20_02766 0.70 -1.2 2,922,389 - MPMX20_02766 0.70 -1.3 2,922,391 + MPMX20_02766 0.70 -1.8 2,922,440 - MPMX20_02766 0.75 -0.1 2,922,458 - MPMX20_02766 0.76 -0.8 2,922,491 + MPMX20_02766 0.80 -2.5 2,922,626 - -0.2 2,922,881 + MPMX20_02767 0.29 +1.1 2,922,892 + MPMX20_02767 0.31 -1.9 2,922,900 - MPMX20_02767 0.32 -1.5 2,922,916 + MPMX20_02767 0.34 +0.5 2,922,916 + MPMX20_02767 0.34 -2.7 2,922,979 + MPMX20_02767 0.45 +0.1 2,922,987 - MPMX20_02767 0.46 -0.6 2,923,001 - MPMX20_02767 0.48 -0.8 2,923,034 - MPMX20_02767 0.54 -1.0 2,923,158 - MPMX20_02767 0.74 -2.6 2,923,242 + MPMX20_02767 0.88 -2.5 2,923,268 - -2.7 2,923,331 - -1.0 2,923,407 - -0.1 2,923,447 - -2.0 2,923,447 - -1.7 2,923,471 + MPMX20_02768 0.14 -1.9 2,923,479 - MPMX20_02768 0.16 -1.5 2,923,526 + MPMX20_02768 0.25 -0.3 2,923,526 + MPMX20_02768 0.25 -2.0 2,923,532 - MPMX20_02768 0.26 -2.1
Or see this region's nucleotide sequence