Experiment: pectate 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS12585 and HMPREF1058_RS12580 are separated by 126 nucleotides HMPREF1058_RS12580 and HMPREF1058_RS12575 are separated by 184 nucleotides HMPREF1058_RS12575 and HMPREF1058_RS12570 are separated by 88 nucleotides HMPREF1058_RS12570 and HMPREF1058_RS12565 are separated by 51 nucleotides
HMPREF1058_RS12585: HMPREF1058_RS12585 - IS1182 family transposase, at 74,854 to 75,257
_RS12585
HMPREF1058_RS12580: HMPREF1058_RS12580 - tRNA-Leu, at 75,384 to 75,467
_RS12580
HMPREF1058_RS12575: HMPREF1058_RS12575 - mannose-1-phosphate guanylyltransferase, at 75,652 to 76,695
_RS12575
HMPREF1058_RS12570: HMPREF1058_RS12570 - HIT family protein, at 76,784 to 77,191
_RS12570
HMPREF1058_RS12565: HMPREF1058_RS12565 - transcription elongation factor GreA, at 77,243 to 77,707
_RS12565
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 75.352 kb on + strand at 75.568 kb on + strand at 75.568 kb on + strand at 75.569 kb on - strand at 75.569 kb on - strand at 75.570 kb on + strand at 75.580 kb on + strand at 75.581 kb on - strand at 75.646 kb on + strand at 75.647 kb on - strand at 75.647 kb on - strand at 75.649 kb on - strand at 75.693 kb on + strand at 75.693 kb on + strand at 75.694 kb on - strand at 75.695 kb on + strand at 75.695 kb on + strand at 75.715 kb on + strand at 75.718 kb on - strand at 75.843 kb on - strand, within HMPREF1058_RS12575 at 75.847 kb on + strand, within HMPREF1058_RS12575 at 75.848 kb on - strand, within HMPREF1058_RS12575 at 75.934 kb on - strand, within HMPREF1058_RS12575 at 75.949 kb on + strand, within HMPREF1058_RS12575 at 75.954 kb on + strand, within HMPREF1058_RS12575 at 76.088 kb on + strand, within HMPREF1058_RS12575 at 76.089 kb on - strand, within HMPREF1058_RS12575 at 76.089 kb on - strand, within HMPREF1058_RS12575 at 76.089 kb on - strand, within HMPREF1058_RS12575 at 76.089 kb on - strand, within HMPREF1058_RS12575 at 76.093 kb on - strand, within HMPREF1058_RS12575 at 76.126 kb on + strand, within HMPREF1058_RS12575 at 76.127 kb on - strand, within HMPREF1058_RS12575 at 76.170 kb on - strand, within HMPREF1058_RS12575 at 76.250 kb on + strand, within HMPREF1058_RS12575 at 76.251 kb on - strand, within HMPREF1058_RS12575 at 76.263 kb on + strand, within HMPREF1058_RS12575 at 76.264 kb on - strand, within HMPREF1058_RS12575 at 76.270 kb on - strand, within HMPREF1058_RS12575 at 76.356 kb on + strand, within HMPREF1058_RS12575 at 76.433 kb on + strand, within HMPREF1058_RS12575 at 76.434 kb on - strand, within HMPREF1058_RS12575 at 76.434 kb on - strand, within HMPREF1058_RS12575 at 76.454 kb on - strand, within HMPREF1058_RS12575 at 76.454 kb on - strand, within HMPREF1058_RS12575 at 76.460 kb on + strand, within HMPREF1058_RS12575 at 76.460 kb on + strand, within HMPREF1058_RS12575 at 76.491 kb on - strand, within HMPREF1058_RS12575 at 76.511 kb on + strand, within HMPREF1058_RS12575 at 76.512 kb on - strand, within HMPREF1058_RS12575 at 76.514 kb on - strand, within HMPREF1058_RS12575 at 76.516 kb on - strand, within HMPREF1058_RS12575 at 76.522 kb on + strand, within HMPREF1058_RS12575 at 76.523 kb on - strand, within HMPREF1058_RS12575 at 76.527 kb on + strand, within HMPREF1058_RS12575 at 76.528 kb on - strand, within HMPREF1058_RS12575 at 76.532 kb on + strand, within HMPREF1058_RS12575 at 76.533 kb on - strand, within HMPREF1058_RS12575 at 76.535 kb on + strand, within HMPREF1058_RS12575 at 76.536 kb on - strand, within HMPREF1058_RS12575 at 76.539 kb on - strand, within HMPREF1058_RS12575 at 76.566 kb on - strand, within HMPREF1058_RS12575 at 76.574 kb on + strand, within HMPREF1058_RS12575 at 76.575 kb on - strand, within HMPREF1058_RS12575 at 76.586 kb on + strand, within HMPREF1058_RS12575 at 76.617 kb on - strand at 76.761 kb on - strand at 76.763 kb on - strand at 76.770 kb on - strand at 76.785 kb on + strand at 76.912 kb on + strand, within HMPREF1058_RS12570 at 76.913 kb on - strand, within HMPREF1058_RS12570 at 76.913 kb on - strand, within HMPREF1058_RS12570 at 76.931 kb on + strand, within HMPREF1058_RS12570 at 76.953 kb on - strand, within HMPREF1058_RS12570 at 77.015 kb on - strand, within HMPREF1058_RS12570 at 77.049 kb on + strand, within HMPREF1058_RS12570 at 77.050 kb on - strand, within HMPREF1058_RS12570 at 77.080 kb on + strand, within HMPREF1058_RS12570 at 77.080 kb on + strand, within HMPREF1058_RS12570 at 77.081 kb on - strand, within HMPREF1058_RS12570 at 77.164 kb on + strand at 77.164 kb on + strand at 77.181 kb on - strand at 77.191 kb on + strand at 77.192 kb on - strand at 77.196 kb on - strand at 77.197 kb on + strand at 77.197 kb on + strand at 77.198 kb on - strand at 77.204 kb on + strand at 77.208 kb on + strand at 77.237 kb on + strand at 77.237 kb on + strand at 77.239 kb on + strand at 77.239 kb on + strand at 77.240 kb on - strand at 77.244 kb on + strand at 77.244 kb on + strand at 77.255 kb on - strand at 77.373 kb on - strand, within HMPREF1058_RS12565 at 77.571 kb on + strand, within HMPREF1058_RS12565
Per-strain Table
Position Strand Gene LocusTag Fraction pectate 2.5 mg/ml (C) remove 75,352 + +1.9 75,568 + -0.2 75,568 + +0.1 75,569 - -0.4 75,569 - -0.6 75,570 + -2.0 75,580 + +1.1 75,581 - +1.9 75,646 + -0.8 75,647 - -0.8 75,647 - -1.8 75,649 - -1.0 75,693 + +0.2 75,693 + -0.6 75,694 - -0.7 75,695 + -0.3 75,695 + +0.6 75,715 + -1.1 75,718 - -1.6 75,843 - HMPREF1058_RS12575 0.18 -1.0 75,847 + HMPREF1058_RS12575 0.19 +0.2 75,848 - HMPREF1058_RS12575 0.19 -0.5 75,934 - HMPREF1058_RS12575 0.27 -1.0 75,949 + HMPREF1058_RS12575 0.28 -0.9 75,954 + HMPREF1058_RS12575 0.29 -1.0 76,088 + HMPREF1058_RS12575 0.42 -1.6 76,089 - HMPREF1058_RS12575 0.42 -0.9 76,089 - HMPREF1058_RS12575 0.42 -1.5 76,089 - HMPREF1058_RS12575 0.42 -0.5 76,089 - HMPREF1058_RS12575 0.42 -0.8 76,093 - HMPREF1058_RS12575 0.42 -0.4 76,126 + HMPREF1058_RS12575 0.45 -0.6 76,127 - HMPREF1058_RS12575 0.45 -0.5 76,170 - HMPREF1058_RS12575 0.50 -0.9 76,250 + HMPREF1058_RS12575 0.57 -1.5 76,251 - HMPREF1058_RS12575 0.57 -0.0 76,263 + HMPREF1058_RS12575 0.59 -0.7 76,264 - HMPREF1058_RS12575 0.59 -0.8 76,270 - HMPREF1058_RS12575 0.59 -0.7 76,356 + HMPREF1058_RS12575 0.67 -1.2 76,433 + HMPREF1058_RS12575 0.75 -1.1 76,434 - HMPREF1058_RS12575 0.75 -0.5 76,434 - HMPREF1058_RS12575 0.75 -0.4 76,454 - HMPREF1058_RS12575 0.77 -0.9 76,454 - HMPREF1058_RS12575 0.77 -0.8 76,460 + HMPREF1058_RS12575 0.77 -0.3 76,460 + HMPREF1058_RS12575 0.77 -0.3 76,491 - HMPREF1058_RS12575 0.80 -0.5 76,511 + HMPREF1058_RS12575 0.82 -1.1 76,512 - HMPREF1058_RS12575 0.82 -1.0 76,514 - HMPREF1058_RS12575 0.83 -0.4 76,516 - HMPREF1058_RS12575 0.83 +0.5 76,522 + HMPREF1058_RS12575 0.83 -1.3 76,523 - HMPREF1058_RS12575 0.83 -1.1 76,527 + HMPREF1058_RS12575 0.84 -0.8 76,528 - HMPREF1058_RS12575 0.84 -0.3 76,532 + HMPREF1058_RS12575 0.84 -0.5 76,533 - HMPREF1058_RS12575 0.84 +0.9 76,535 + HMPREF1058_RS12575 0.85 -1.7 76,536 - HMPREF1058_RS12575 0.85 -0.3 76,539 - HMPREF1058_RS12575 0.85 -0.7 76,566 - HMPREF1058_RS12575 0.88 -1.8 76,574 + HMPREF1058_RS12575 0.88 -0.8 76,575 - HMPREF1058_RS12575 0.88 -0.9 76,586 + HMPREF1058_RS12575 0.89 -1.0 76,617 - -0.4 76,761 - +0.1 76,763 - -1.1 76,770 - +0.8 76,785 + +0.4 76,912 + HMPREF1058_RS12570 0.31 +1.0 76,913 - HMPREF1058_RS12570 0.32 +1.9 76,913 - HMPREF1058_RS12570 0.32 -0.2 76,931 + HMPREF1058_RS12570 0.36 -0.6 76,953 - HMPREF1058_RS12570 0.41 +1.4 77,015 - HMPREF1058_RS12570 0.57 -0.8 77,049 + HMPREF1058_RS12570 0.65 -0.3 77,050 - HMPREF1058_RS12570 0.65 -0.2 77,080 + HMPREF1058_RS12570 0.73 -0.2 77,080 + HMPREF1058_RS12570 0.73 +0.1 77,081 - HMPREF1058_RS12570 0.73 +0.1 77,164 + -0.8 77,164 + +0.5 77,181 - -0.1 77,191 + -0.2 77,192 - +0.8 77,196 - -0.7 77,197 + +0.6 77,197 + +1.7 77,198 - +0.7 77,204 + -0.6 77,208 + +0.0 77,237 + -0.6 77,237 + -0.4 77,239 + -0.5 77,239 + +0.6 77,240 - +0.3 77,244 + -0.5 77,244 + +0.2 77,255 - -0.3 77,373 - HMPREF1058_RS12565 0.28 -1.1 77,571 + HMPREF1058_RS12565 0.71 -0.9
Or see this region's nucleotide sequence