Experiment: pectate 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06175 and HMPREF1058_RS06170 are separated by 9 nucleotides HMPREF1058_RS06170 and HMPREF1058_RS06165 are separated by 41 nucleotides HMPREF1058_RS06165 and HMPREF1058_RS06160 overlap by 1 nucleotides
HMPREF1058_RS06175: HMPREF1058_RS06175 - imidazole glycerol phosphate synthase subunit HisF, at 541,827 to 542,582
_RS06175
HMPREF1058_RS06170: HMPREF1058_RS06170 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 542,592 to 543,311
_RS06170
HMPREF1058_RS06165: HMPREF1058_RS06165 - imidazole glycerol phosphate synthase subunit HisH, at 543,353 to 543,943
_RS06165
HMPREF1058_RS06160: HMPREF1058_RS06160 - formyltetrahydrofolate deformylase, at 543,943 to 544,800
_RS06160
Position (kb) 543
544 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 542.467 kb on + strand, within HMPREF1058_RS06175 at 542.471 kb on + strand, within HMPREF1058_RS06175 at 542.471 kb on + strand, within HMPREF1058_RS06175 at 542.529 kb on + strand at 542.594 kb on - strand at 542.594 kb on - strand at 542.635 kb on + strand at 542.697 kb on + strand, within HMPREF1058_RS06170 at 542.815 kb on - strand, within HMPREF1058_RS06170 at 542.815 kb on - strand, within HMPREF1058_RS06170 at 542.838 kb on - strand, within HMPREF1058_RS06170 at 542.841 kb on - strand, within HMPREF1058_RS06170 at 542.842 kb on + strand, within HMPREF1058_RS06170 at 542.843 kb on - strand, within HMPREF1058_RS06170 at 543.010 kb on - strand, within HMPREF1058_RS06170 at 543.228 kb on + strand, within HMPREF1058_RS06170 at 543.229 kb on - strand, within HMPREF1058_RS06170 at 543.244 kb on + strand at 543.300 kb on + strand at 543.301 kb on - strand at 543.301 kb on - strand at 543.315 kb on + strand at 543.316 kb on - strand at 543.328 kb on - strand at 543.328 kb on - strand at 543.335 kb on + strand at 543.340 kb on - strand at 543.340 kb on - strand at 543.342 kb on - strand at 543.467 kb on - strand, within HMPREF1058_RS06165 at 543.467 kb on - strand, within HMPREF1058_RS06165 at 543.507 kb on + strand, within HMPREF1058_RS06165 at 543.508 kb on - strand, within HMPREF1058_RS06165 at 543.519 kb on - strand, within HMPREF1058_RS06165 at 543.519 kb on - strand, within HMPREF1058_RS06165 at 543.638 kb on + strand, within HMPREF1058_RS06165 at 543.638 kb on + strand, within HMPREF1058_RS06165 at 543.722 kb on + strand, within HMPREF1058_RS06165 at 543.726 kb on - strand, within HMPREF1058_RS06165 at 543.737 kb on + strand, within HMPREF1058_RS06165 at 543.737 kb on + strand, within HMPREF1058_RS06165 at 543.746 kb on - strand, within HMPREF1058_RS06165 at 543.839 kb on + strand, within HMPREF1058_RS06165 at 543.840 kb on - strand, within HMPREF1058_RS06165 at 543.903 kb on + strand at 543.954 kb on + strand at 543.955 kb on - strand at 543.972 kb on - strand at 543.993 kb on + strand at 543.993 kb on + strand at 544.048 kb on + strand, within HMPREF1058_RS06160 at 544.087 kb on + strand, within HMPREF1058_RS06160 at 544.088 kb on - strand, within HMPREF1058_RS06160 at 544.225 kb on + strand, within HMPREF1058_RS06160 at 544.282 kb on - strand, within HMPREF1058_RS06160 at 544.431 kb on + strand, within HMPREF1058_RS06160 at 544.435 kb on + strand, within HMPREF1058_RS06160 at 544.435 kb on + strand, within HMPREF1058_RS06160 at 544.488 kb on + strand, within HMPREF1058_RS06160 at 544.541 kb on - strand, within HMPREF1058_RS06160 at 544.586 kb on + strand, within HMPREF1058_RS06160 at 544.602 kb on - strand, within HMPREF1058_RS06160 at 544.639 kb on + strand, within HMPREF1058_RS06160 at 544.689 kb on - strand, within HMPREF1058_RS06160 at 544.726 kb on - strand at 544.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction pectate 2.5 mg/ml (C) remove 542,467 + HMPREF1058_RS06175 0.85 -1.9 542,471 + HMPREF1058_RS06175 0.85 -4.1 542,471 + HMPREF1058_RS06175 0.85 -2.0 542,529 + -2.2 542,594 - +2.8 542,594 - +0.1 542,635 + -2.6 542,697 + HMPREF1058_RS06170 0.15 -1.8 542,815 - HMPREF1058_RS06170 0.31 -0.9 542,815 - HMPREF1058_RS06170 0.31 -5.1 542,838 - HMPREF1058_RS06170 0.34 -1.4 542,841 - HMPREF1058_RS06170 0.35 -1.4 542,842 + HMPREF1058_RS06170 0.35 -0.5 542,843 - HMPREF1058_RS06170 0.35 -2.4 543,010 - HMPREF1058_RS06170 0.58 -3.0 543,228 + HMPREF1058_RS06170 0.88 -1.1 543,229 - HMPREF1058_RS06170 0.88 -1.8 543,244 + -3.5 543,300 + -1.6 543,301 - -2.1 543,301 - -2.5 543,315 + -2.2 543,316 - -2.3 543,328 - -1.5 543,328 - +0.6 543,335 + -2.4 543,340 - -1.2 543,340 - +0.4 543,342 - +0.4 543,467 - HMPREF1058_RS06165 0.19 -1.4 543,467 - HMPREF1058_RS06165 0.19 -0.1 543,507 + HMPREF1058_RS06165 0.26 -0.8 543,508 - HMPREF1058_RS06165 0.26 -0.6 543,519 - HMPREF1058_RS06165 0.28 -0.9 543,519 - HMPREF1058_RS06165 0.28 -0.5 543,638 + HMPREF1058_RS06165 0.48 -2.8 543,638 + HMPREF1058_RS06165 0.48 -2.7 543,722 + HMPREF1058_RS06165 0.62 -3.2 543,726 - HMPREF1058_RS06165 0.63 +0.6 543,737 + HMPREF1058_RS06165 0.65 -3.5 543,737 + HMPREF1058_RS06165 0.65 -2.9 543,746 - HMPREF1058_RS06165 0.66 +0.5 543,839 + HMPREF1058_RS06165 0.82 -0.8 543,840 - HMPREF1058_RS06165 0.82 -0.8 543,903 + -0.6 543,954 + -3.8 543,955 - +0.7 543,972 - +0.7 543,993 + -1.0 543,993 + -0.6 544,048 + HMPREF1058_RS06160 0.12 -0.2 544,087 + HMPREF1058_RS06160 0.17 -0.7 544,088 - HMPREF1058_RS06160 0.17 +0.2 544,225 + HMPREF1058_RS06160 0.33 -0.0 544,282 - HMPREF1058_RS06160 0.40 -0.8 544,431 + HMPREF1058_RS06160 0.57 +0.1 544,435 + HMPREF1058_RS06160 0.57 -0.7 544,435 + HMPREF1058_RS06160 0.57 -0.3 544,488 + HMPREF1058_RS06160 0.64 +0.3 544,541 - HMPREF1058_RS06160 0.70 -0.7 544,586 + HMPREF1058_RS06160 0.75 -0.1 544,602 - HMPREF1058_RS06160 0.77 -0.2 544,639 + HMPREF1058_RS06160 0.81 -1.6 544,689 - HMPREF1058_RS06160 0.87 +0.7 544,726 - +0.7 544,795 - -0.5
Or see this region's nucleotide sequence