Experiment: pectate 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS02495 and HMPREF1058_RS02490 are separated by 41 nucleotides HMPREF1058_RS02490 and HMPREF1058_RS02485 are separated by 35 nucleotides HMPREF1058_RS02485 and HMPREF1058_RS02480 are separated by 5 nucleotides
HMPREF1058_RS02495: HMPREF1058_RS02495 - acyl-CoA dehydrogenase family protein, at 169,412 to 171,118
_RS02495
HMPREF1058_RS02490: HMPREF1058_RS02490 - four helix bundle protein, at 171,160 to 171,540
_RS02490
HMPREF1058_RS02485: HMPREF1058_RS02485 - electron transfer flavoprotein subunit alpha/FixB family protein, at 171,576 to 172,595
_RS02485
HMPREF1058_RS02480: HMPREF1058_RS02480 - electron transfer flavoprotein subunit beta/FixA family protein, at 172,601 to 173,494
_RS02480
Position (kb)
171
172
173 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 170.670 kb on + strand, within HMPREF1058_RS02495 at 170.671 kb on - strand, within HMPREF1058_RS02495 at 170.989 kb on - strand at 171.020 kb on - strand at 171.096 kb on - strand at 171.129 kb on - strand at 171.132 kb on + strand at 171.132 kb on + strand at 171.132 kb on + strand at 171.145 kb on - strand at 171.145 kb on - strand at 171.151 kb on + strand at 171.161 kb on + strand at 171.161 kb on + strand at 171.161 kb on + strand at 171.162 kb on - strand at 171.239 kb on - strand, within HMPREF1058_RS02490 at 171.239 kb on - strand, within HMPREF1058_RS02490 at 171.558 kb on - strand at 171.561 kb on + strand at 171.562 kb on - strand at 171.562 kb on - strand at 171.562 kb on - strand at 171.562 kb on - strand at 171.596 kb on - strand at 171.598 kb on + strand at 171.642 kb on + strand at 171.643 kb on - strand at 171.997 kb on + strand, within HMPREF1058_RS02485 at 171.997 kb on + strand, within HMPREF1058_RS02485 at 171.997 kb on + strand, within HMPREF1058_RS02485 at 171.997 kb on + strand, within HMPREF1058_RS02485 at 171.998 kb on - strand, within HMPREF1058_RS02485 at 172.172 kb on - strand, within HMPREF1058_RS02485 at 172.273 kb on - strand, within HMPREF1058_RS02485 at 172.380 kb on - strand, within HMPREF1058_RS02485 at 172.380 kb on - strand, within HMPREF1058_RS02485 at 172.382 kb on - strand at 172.407 kb on + strand, within HMPREF1058_RS02485 at 172.437 kb on + strand, within HMPREF1058_RS02485 at 172.510 kb on - strand at 172.578 kb on + strand at 172.578 kb on + strand at 172.584 kb on + strand at 172.608 kb on - strand at 172.612 kb on + strand at 172.613 kb on - strand at 172.764 kb on - strand, within HMPREF1058_RS02480 at 172.814 kb on + strand, within HMPREF1058_RS02480 at 172.814 kb on + strand, within HMPREF1058_RS02480 at 172.814 kb on + strand, within HMPREF1058_RS02480 at 172.815 kb on - strand, within HMPREF1058_RS02480 at 173.057 kb on - strand, within HMPREF1058_RS02480 at 173.168 kb on - strand, within HMPREF1058_RS02480 at 173.235 kb on + strand, within HMPREF1058_RS02480 at 173.236 kb on - strand, within HMPREF1058_RS02480 at 173.238 kb on - strand, within HMPREF1058_RS02480 at 173.475 kb on - strand at 173.477 kb on + strand at 173.496 kb on + strand at 173.497 kb on - strand at 173.507 kb on - strand at 173.516 kb on + strand at 173.517 kb on - strand at 173.517 kb on - strand at 173.517 kb on - strand at 173.580 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pectate 2.5 mg/ml (C) remove 170,670 + HMPREF1058_RS02495 0.74 -0.2 170,671 - HMPREF1058_RS02495 0.74 -0.1 170,989 - -1.5 171,020 - -2.6 171,096 - +0.3 171,129 - +0.5 171,132 + +0.0 171,132 + +2.0 171,132 + -0.7 171,145 - +0.2 171,145 - +1.4 171,151 + -0.2 171,161 + +3.0 171,161 + +0.3 171,161 + +0.4 171,162 - +0.9 171,239 - HMPREF1058_RS02490 0.21 +0.2 171,239 - HMPREF1058_RS02490 0.21 +0.5 171,558 - +0.1 171,561 + -0.2 171,562 - +0.8 171,562 - +1.3 171,562 - +0.5 171,562 - +1.1 171,596 - -0.4 171,598 + +0.4 171,642 + +1.5 171,643 - -1.2 171,997 + HMPREF1058_RS02485 0.41 +0.7 171,997 + HMPREF1058_RS02485 0.41 -1.0 171,997 + HMPREF1058_RS02485 0.41 -2.0 171,997 + HMPREF1058_RS02485 0.41 -1.1 171,998 - HMPREF1058_RS02485 0.41 -0.3 172,172 - HMPREF1058_RS02485 0.58 -0.1 172,273 - HMPREF1058_RS02485 0.68 -1.6 172,380 - HMPREF1058_RS02485 0.79 -0.4 172,380 - HMPREF1058_RS02485 0.79 +0.7 172,382 - +1.4 172,407 + HMPREF1058_RS02485 0.81 +0.0 172,437 + HMPREF1058_RS02485 0.84 -0.4 172,510 - +1.0 172,578 + +0.0 172,578 + +3.0 172,584 + -1.2 172,608 - +1.0 172,612 + -0.6 172,613 - -0.9 172,764 - HMPREF1058_RS02480 0.18 -0.1 172,814 + HMPREF1058_RS02480 0.24 -1.5 172,814 + HMPREF1058_RS02480 0.24 +0.5 172,814 + HMPREF1058_RS02480 0.24 +0.1 172,815 - HMPREF1058_RS02480 0.24 -1.3 173,057 - HMPREF1058_RS02480 0.51 -0.7 173,168 - HMPREF1058_RS02480 0.63 -0.9 173,235 + HMPREF1058_RS02480 0.71 -1.2 173,236 - HMPREF1058_RS02480 0.71 +1.0 173,238 - HMPREF1058_RS02480 0.71 -1.0 173,475 - +0.7 173,477 + -0.5 173,496 + +1.0 173,497 - -1.3 173,507 - +0.4 173,516 + +1.0 173,517 - +0.7 173,517 - +0.2 173,517 - +0.2 173,580 + +0.7
Or see this region's nucleotide sequence