Experiment: pectate 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS21380 and HMPREF1058_RS19065 are separated by 214 nucleotides HMPREF1058_RS19065 and HMPREF1058_RS19060 overlap by 4 nucleotides HMPREF1058_RS19060 and HMPREF1058_RS0119250 are separated by 13 nucleotides HMPREF1058_RS0119250 and HMPREF1058_RS19055 are separated by 75 nucleotides HMPREF1058_RS19055 and HMPREF1058_RS19050 are separated by 6 nucleotides HMPREF1058_RS19050 and HMPREF1058_RS20335 overlap by 11 nucleotides HMPREF1058_RS20335 and HMPREF1058_RS19045 are separated by 5 nucleotides
HMPREF1058_RS21380: HMPREF1058_RS21380 - hypothetical protein, at 27,326 to 27,499
_RS21380
HMPREF1058_RS19065: HMPREF1058_RS19065 - helix-turn-helix domain-containing protein, at 27,714 to 28,025
_RS19065
HMPREF1058_RS19060: HMPREF1058_RS19060 - type II toxin-antitoxin system RelE/ParE family toxin, at 28,022 to 28,387
_RS19060
HMPREF1058_RS0119250: HMPREF1058_RS0119250 - hypothetical protein, at 28,401 to 28,688
_RS0119250
HMPREF1058_RS19055: HMPREF1058_RS19055 - hypothetical protein, at 28,764 to 29,039
_RS19055
HMPREF1058_RS19050: HMPREF1058_RS19050 - hypothetical protein, at 29,046 to 29,246
_RS19050
HMPREF1058_RS20335: HMPREF1058_RS20335 - DpnD/PcfM family protein, at 29,236 to 29,412
_RS20335
HMPREF1058_RS19045: HMPREF1058_RS19045 - hypothetical protein, at 29,418 to 29,828
_RS19045
Position (kb)
28
29 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 27.472 kb on - strand, within HMPREF1058_RS21380 at 27.481 kb on + strand, within HMPREF1058_RS21380 at 27.514 kb on - strand at 27.514 kb on - strand at 27.523 kb on + strand at 27.524 kb on - strand at 27.526 kb on + strand at 27.527 kb on - strand at 27.527 kb on - strand at 28.128 kb on - strand, within HMPREF1058_RS19060 at 28.204 kb on - strand, within HMPREF1058_RS19060 at 28.206 kb on - strand, within HMPREF1058_RS19060 at 28.230 kb on - strand, within HMPREF1058_RS19060 at 28.230 kb on - strand, within HMPREF1058_RS19060 at 28.232 kb on - strand, within HMPREF1058_RS19060 at 28.234 kb on - strand, within HMPREF1058_RS19060 at 28.234 kb on - strand, within HMPREF1058_RS19060 at 28.239 kb on - strand, within HMPREF1058_RS19060 at 28.252 kb on - strand, within HMPREF1058_RS19060 at 28.252 kb on - strand, within HMPREF1058_RS19060 at 28.252 kb on - strand, within HMPREF1058_RS19060 at 28.318 kb on - strand, within HMPREF1058_RS19060 at 28.356 kb on - strand at 28.411 kb on - strand at 28.413 kb on - strand at 28.439 kb on + strand, within HMPREF1058_RS0119250 at 28.439 kb on + strand, within HMPREF1058_RS0119250 at 28.439 kb on + strand, within HMPREF1058_RS0119250 at 28.440 kb on - strand, within HMPREF1058_RS0119250 at 28.479 kb on + strand, within HMPREF1058_RS0119250 at 28.532 kb on - strand, within HMPREF1058_RS0119250 at 28.713 kb on - strand at 28.737 kb on + strand at 28.757 kb on + strand at 28.789 kb on + strand at 28.817 kb on - strand, within HMPREF1058_RS19055 at 28.857 kb on - strand, within HMPREF1058_RS19055 at 28.877 kb on - strand, within HMPREF1058_RS19055 at 28.887 kb on - strand, within HMPREF1058_RS19055 at 28.900 kb on + strand, within HMPREF1058_RS19055 at 28.901 kb on - strand, within HMPREF1058_RS19055 at 28.998 kb on + strand, within HMPREF1058_RS19055 at 28.999 kb on - strand, within HMPREF1058_RS19055 at 28.999 kb on - strand at 29.235 kb on - strand at 29.293 kb on - strand, within HMPREF1058_RS20335 at 29.369 kb on - strand, within HMPREF1058_RS20335 at 29.376 kb on + strand, within HMPREF1058_RS20335 at 29.389 kb on + strand, within HMPREF1058_RS20335 at 29.389 kb on + strand, within HMPREF1058_RS20335 at 29.428 kb on - strand at 29.481 kb on - strand, within HMPREF1058_RS19045 at 29.508 kb on - strand, within HMPREF1058_RS19045 at 29.538 kb on + strand, within HMPREF1058_RS19045 at 29.538 kb on + strand, within HMPREF1058_RS19045 at 29.559 kb on - strand, within HMPREF1058_RS19045 at 29.577 kb on + strand, within HMPREF1058_RS19045 at 29.599 kb on + strand, within HMPREF1058_RS19045 at 29.668 kb on - strand, within HMPREF1058_RS19045 at 29.668 kb on - strand, within HMPREF1058_RS19045
Per-strain Table
Position Strand Gene LocusTag Fraction pectate 2.5 mg/ml (C) remove 27,472 - HMPREF1058_RS21380 0.84 -0.5 27,481 + HMPREF1058_RS21380 0.89 -0.2 27,514 - -0.2 27,514 - -0.2 27,523 + -2.6 27,524 - -0.3 27,526 + -0.7 27,527 - +1.0 27,527 - +0.4 28,128 - HMPREF1058_RS19060 0.29 +0.3 28,204 - HMPREF1058_RS19060 0.50 +0.4 28,206 - HMPREF1058_RS19060 0.50 -0.4 28,230 - HMPREF1058_RS19060 0.57 -0.7 28,230 - HMPREF1058_RS19060 0.57 +0.1 28,232 - HMPREF1058_RS19060 0.57 -0.2 28,234 - HMPREF1058_RS19060 0.58 -0.3 28,234 - HMPREF1058_RS19060 0.58 -0.2 28,239 - HMPREF1058_RS19060 0.59 -0.9 28,252 - HMPREF1058_RS19060 0.63 +0.1 28,252 - HMPREF1058_RS19060 0.63 -1.2 28,252 - HMPREF1058_RS19060 0.63 -1.3 28,318 - HMPREF1058_RS19060 0.81 -0.6 28,356 - +1.4 28,411 - -1.1 28,413 - -0.2 28,439 + HMPREF1058_RS0119250 0.13 -0.9 28,439 + HMPREF1058_RS0119250 0.13 -0.2 28,439 + HMPREF1058_RS0119250 0.13 -1.7 28,440 - HMPREF1058_RS0119250 0.14 -0.8 28,479 + HMPREF1058_RS0119250 0.27 +0.0 28,532 - HMPREF1058_RS0119250 0.45 -0.5 28,713 - -1.0 28,737 + +0.2 28,757 + +2.0 28,789 + -0.0 28,817 - HMPREF1058_RS19055 0.19 -0.4 28,857 - HMPREF1058_RS19055 0.34 -0.3 28,877 - HMPREF1058_RS19055 0.41 -0.3 28,887 - HMPREF1058_RS19055 0.45 +0.3 28,900 + HMPREF1058_RS19055 0.49 +0.0 28,901 - HMPREF1058_RS19055 0.50 +0.5 28,998 + HMPREF1058_RS19055 0.85 +1.6 28,999 - HMPREF1058_RS19055 0.85 +0.5 28,999 - +0.2 29,235 - +0.6 29,293 - HMPREF1058_RS20335 0.32 +1.3 29,369 - HMPREF1058_RS20335 0.75 -0.6 29,376 + HMPREF1058_RS20335 0.79 -0.9 29,389 + HMPREF1058_RS20335 0.86 -1.4 29,389 + HMPREF1058_RS20335 0.86 +0.1 29,428 - -0.6 29,481 - HMPREF1058_RS19045 0.15 -0.6 29,508 - HMPREF1058_RS19045 0.22 -0.4 29,538 + HMPREF1058_RS19045 0.29 +0.3 29,538 + HMPREF1058_RS19045 0.29 +0.0 29,559 - HMPREF1058_RS19045 0.34 -0.8 29,577 + HMPREF1058_RS19045 0.39 -0.5 29,599 + HMPREF1058_RS19045 0.44 -0.7 29,668 - HMPREF1058_RS19045 0.61 +0.8 29,668 - HMPREF1058_RS19045 0.61 -0.0
Or see this region's nucleotide sequence