Experiment: polygalacturonic 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS21045 and HMPREF1058_RS04435 are separated by 157 nucleotides HMPREF1058_RS04435 and HMPREF1058_RS21050 are separated by 253 nucleotides HMPREF1058_RS21050 and HMPREF1058_RS04430 are separated by 124 nucleotides HMPREF1058_RS04430 and HMPREF1058_RS04425 are separated by 912 nucleotides
HMPREF1058_RS21045: HMPREF1058_RS21045 - hypothetical protein, at 36,125 to 36,364
_RS21045
HMPREF1058_RS04435: HMPREF1058_RS04435 - hypothetical protein, at 36,522 to 36,779
_RS04435
HMPREF1058_RS21050: HMPREF1058_RS21050 - transposase, at 37,033 to 37,196
_RS21050
HMPREF1058_RS04430: HMPREF1058_RS04430 - alpha-1,2-fucosyltransferase, at 37,321 to 38,166
_RS04430
HMPREF1058_RS04425: HMPREF1058_RS04425 - mechanosensitive ion channel, at 39,079 to 39,924
_RS04425
Position (kb) 37
38
39 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9
10 at 36.480 kb on + strand at 36.489 kb on - strand at 36.499 kb on + strand at 36.500 kb on + strand at 36.501 kb on - strand at 36.502 kb on + strand at 36.514 kb on - strand at 36.521 kb on + strand at 36.527 kb on + strand at 36.528 kb on - strand at 36.529 kb on + strand at 36.599 kb on + strand, within HMPREF1058_RS04435 at 36.614 kb on + strand, within HMPREF1058_RS04435 at 36.638 kb on - strand, within HMPREF1058_RS04435 at 36.643 kb on - strand, within HMPREF1058_RS04435 at 36.652 kb on + strand, within HMPREF1058_RS04435 at 36.653 kb on - strand, within HMPREF1058_RS04435 at 36.653 kb on - strand, within HMPREF1058_RS04435 at 36.679 kb on + strand, within HMPREF1058_RS04435 at 36.680 kb on - strand, within HMPREF1058_RS04435 at 36.680 kb on - strand, within HMPREF1058_RS04435 at 36.708 kb on + strand, within HMPREF1058_RS04435 at 36.799 kb on + strand at 36.802 kb on - strand at 36.938 kb on + strand at 36.939 kb on - strand at 37.178 kb on + strand, within HMPREF1058_RS21050 at 37.178 kb on + strand, within HMPREF1058_RS21050 at 37.181 kb on + strand at 37.225 kb on + strand at 37.312 kb on + strand at 37.312 kb on + strand at 37.355 kb on + strand at 37.356 kb on - strand at 37.452 kb on + strand, within HMPREF1058_RS04430 at 37.535 kb on + strand, within HMPREF1058_RS04430 at 37.536 kb on - strand, within HMPREF1058_RS04430 at 37.537 kb on + strand, within HMPREF1058_RS04430 at 37.593 kb on + strand, within HMPREF1058_RS04430 at 37.649 kb on + strand, within HMPREF1058_RS04430 at 37.650 kb on - strand, within HMPREF1058_RS04430 at 37.650 kb on - strand, within HMPREF1058_RS04430 at 37.650 kb on - strand, within HMPREF1058_RS04430 at 37.655 kb on - strand, within HMPREF1058_RS04430 at 37.663 kb on + strand, within HMPREF1058_RS04430 at 37.664 kb on - strand, within HMPREF1058_RS04430 at 37.716 kb on + strand, within HMPREF1058_RS04430 at 37.721 kb on - strand, within HMPREF1058_RS04430 at 37.721 kb on - strand, within HMPREF1058_RS04430 at 37.765 kb on - strand, within HMPREF1058_RS04430 at 37.912 kb on - strand, within HMPREF1058_RS04430 at 37.935 kb on - strand, within HMPREF1058_RS04430 at 37.987 kb on - strand, within HMPREF1058_RS04430 at 38.046 kb on + strand, within HMPREF1058_RS04430 at 38.048 kb on + strand, within HMPREF1058_RS04430 at 38.071 kb on + strand, within HMPREF1058_RS04430 at 38.073 kb on + strand, within HMPREF1058_RS04430 at 38.087 kb on + strand at 38.155 kb on + strand at 38.197 kb on + strand at 38.313 kb on + strand at 38.324 kb on + strand at 38.327 kb on - strand at 38.329 kb on - strand at 38.389 kb on - strand at 38.393 kb on + strand at 38.393 kb on + strand at 38.394 kb on - strand at 38.425 kb on - strand at 38.674 kb on - strand at 38.726 kb on - strand at 38.730 kb on - strand at 38.730 kb on - strand at 38.777 kb on + strand at 38.780 kb on + strand at 38.780 kb on + strand at 38.781 kb on - strand at 38.781 kb on - strand at 38.781 kb on - strand at 38.782 kb on + strand at 38.782 kb on + strand at 38.782 kb on + strand at 38.783 kb on - strand at 38.783 kb on - strand at 38.832 kb on - strand at 38.832 kb on - strand at 38.842 kb on - strand at 38.866 kb on - strand at 38.874 kb on + strand at 38.915 kb on + strand at 39.003 kb on + strand at 39.004 kb on - strand at 39.086 kb on + strand at 39.090 kb on - strand at 39.144 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction polygalacturonic 2.5 mg/ml (C) remove 36,480 + +0.2 36,489 - +2.6 36,499 + +1.0 36,500 + -0.7 36,501 - +1.0 36,502 + +0.7 36,514 - -1.9 36,521 + -0.5 36,527 + +1.6 36,528 - +2.4 36,529 + +0.6 36,599 + HMPREF1058_RS04435 0.30 +0.0 36,614 + HMPREF1058_RS04435 0.36 +0.1 36,638 - HMPREF1058_RS04435 0.45 +3.1 36,643 - HMPREF1058_RS04435 0.47 -0.1 36,652 + HMPREF1058_RS04435 0.50 -0.4 36,653 - HMPREF1058_RS04435 0.51 -1.8 36,653 - HMPREF1058_RS04435 0.51 +1.0 36,679 + HMPREF1058_RS04435 0.61 -0.2 36,680 - HMPREF1058_RS04435 0.61 +0.6 36,680 - HMPREF1058_RS04435 0.61 +2.5 36,708 + HMPREF1058_RS04435 0.72 +0.4 36,799 + +1.0 36,802 - +1.8 36,938 + +2.0 36,939 - +0.4 37,178 + HMPREF1058_RS21050 0.88 -1.4 37,178 + HMPREF1058_RS21050 0.88 +0.9 37,181 + -0.4 37,225 + +0.2 37,312 + -2.1 37,312 + -2.1 37,355 + +1.0 37,356 - +2.9 37,452 + HMPREF1058_RS04430 0.15 -2.0 37,535 + HMPREF1058_RS04430 0.25 +5.0 37,536 - HMPREF1058_RS04430 0.25 +3.4 37,537 + HMPREF1058_RS04430 0.26 +1.5 37,593 + HMPREF1058_RS04430 0.32 +0.6 37,649 + HMPREF1058_RS04430 0.39 +0.0 37,650 - HMPREF1058_RS04430 0.39 +0.7 37,650 - HMPREF1058_RS04430 0.39 +2.1 37,650 - HMPREF1058_RS04430 0.39 +3.4 37,655 - HMPREF1058_RS04430 0.39 +5.2 37,663 + HMPREF1058_RS04430 0.40 +0.5 37,664 - HMPREF1058_RS04430 0.41 +1.4 37,716 + HMPREF1058_RS04430 0.47 +0.2 37,721 - HMPREF1058_RS04430 0.47 +0.8 37,721 - HMPREF1058_RS04430 0.47 -0.2 37,765 - HMPREF1058_RS04430 0.52 -0.5 37,912 - HMPREF1058_RS04430 0.70 +0.2 37,935 - HMPREF1058_RS04430 0.73 +1.9 37,987 - HMPREF1058_RS04430 0.79 +0.0 38,046 + HMPREF1058_RS04430 0.86 +10.6 38,048 + HMPREF1058_RS04430 0.86 -0.8 38,071 + HMPREF1058_RS04430 0.89 -1.4 38,073 + HMPREF1058_RS04430 0.89 +0.4 38,087 + +0.0 38,155 + +1.7 38,197 + -0.3 38,313 + +1.2 38,324 + +1.0 38,327 - -0.4 38,329 - +0.9 38,389 - +1.5 38,393 + +0.7 38,393 + -0.0 38,394 - +1.8 38,425 - +0.4 38,674 - +0.6 38,726 - -1.4 38,730 - -0.3 38,730 - -0.5 38,777 + -1.1 38,780 + +0.6 38,780 + +0.0 38,781 - -0.5 38,781 - +0.0 38,781 - -0.1 38,782 + +0.4 38,782 + -2.3 38,782 + -2.5 38,783 - -0.7 38,783 - -0.3 38,832 - +0.6 38,832 - +2.1 38,842 - +0.2 38,866 - -1.4 38,874 + -1.2 38,915 + +0.0 39,003 + -2.3 39,004 - +3.4 39,086 + +0.0 39,090 - -2.2 39,144 - -1.1
Or see this region's nucleotide sequence