Experiment: Rhamnogalacturonan from soy bean pectic fibre 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS16760 and HMPREF1058_RS16755 are separated by 92 nucleotides HMPREF1058_RS16755 and HMPREF1058_RS16750 are separated by 18 nucleotides HMPREF1058_RS16750 and HMPREF1058_RS16745 are separated by 7 nucleotides HMPREF1058_RS16745 and HMPREF1058_RS16740 overlap by 4 nucleotides
HMPREF1058_RS16760: HMPREF1058_RS16760 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 603,092 to 604,036
_RS16760
HMPREF1058_RS16755: HMPREF1058_RS16755 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 604,129 to 604,866
_RS16755
HMPREF1058_RS16750: HMPREF1058_RS16750 - shikimate dehydrogenase, at 604,885 to 605,625
_RS16750
HMPREF1058_RS16745: HMPREF1058_RS16745 - alpha/beta hydrolase, at 605,633 to 606,583
_RS16745
HMPREF1058_RS16740: HMPREF1058_RS16740 - TPM domain-containing protein, at 606,580 to 607,500
_RS16740
Position (kb) 604
605
606 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 603.999 kb on + strand at 604.059 kb on + strand at 604.314 kb on + strand, within HMPREF1058_RS16755 at 604.314 kb on + strand, within HMPREF1058_RS16755 at 604.436 kb on + strand, within HMPREF1058_RS16755 at 604.521 kb on + strand, within HMPREF1058_RS16755 at 604.658 kb on + strand, within HMPREF1058_RS16755 at 604.660 kb on + strand, within HMPREF1058_RS16755 at 605.066 kb on + strand, within HMPREF1058_RS16750 at 605.066 kb on + strand, within HMPREF1058_RS16750 at 605.071 kb on + strand, within HMPREF1058_RS16750 at 605.180 kb on + strand at 605.398 kb on - strand at 605.427 kb on + strand, within HMPREF1058_RS16750 at 605.428 kb on - strand, within HMPREF1058_RS16750 at 605.431 kb on + strand, within HMPREF1058_RS16750 at 605.432 kb on - strand at 605.611 kb on - strand at 605.612 kb on + strand at 605.612 kb on + strand at 605.687 kb on - strand at 605.688 kb on + strand at 605.689 kb on - strand at 605.689 kb on - strand at 605.689 kb on - strand at 605.712 kb on - strand at 605.776 kb on + strand, within HMPREF1058_RS16745 at 605.779 kb on - strand, within HMPREF1058_RS16745 at 605.789 kb on + strand, within HMPREF1058_RS16745 at 605.800 kb on - strand, within HMPREF1058_RS16745 at 605.815 kb on + strand, within HMPREF1058_RS16745 at 605.851 kb on + strand, within HMPREF1058_RS16745 at 605.851 kb on + strand, within HMPREF1058_RS16745 at 605.855 kb on + strand, within HMPREF1058_RS16745 at 605.872 kb on - strand, within HMPREF1058_RS16745 at 605.885 kb on + strand, within HMPREF1058_RS16745 at 605.885 kb on + strand, within HMPREF1058_RS16745 at 605.886 kb on - strand, within HMPREF1058_RS16745 at 605.889 kb on - strand, within HMPREF1058_RS16745 at 605.926 kb on + strand, within HMPREF1058_RS16745 at 605.929 kb on - strand, within HMPREF1058_RS16745 at 605.932 kb on - strand, within HMPREF1058_RS16745 at 605.970 kb on + strand, within HMPREF1058_RS16745 at 605.979 kb on + strand, within HMPREF1058_RS16745 at 606.013 kb on - strand, within HMPREF1058_RS16745 at 606.141 kb on + strand, within HMPREF1058_RS16745 at 606.142 kb on - strand, within HMPREF1058_RS16745 at 606.173 kb on - strand, within HMPREF1058_RS16745 at 606.235 kb on + strand, within HMPREF1058_RS16745 at 606.235 kb on + strand, within HMPREF1058_RS16745 at 606.425 kb on + strand, within HMPREF1058_RS16745 at 606.425 kb on + strand, within HMPREF1058_RS16745 at 606.426 kb on - strand, within HMPREF1058_RS16745 at 606.484 kb on - strand, within HMPREF1058_RS16745 at 606.518 kb on + strand at 606.519 kb on - strand at 606.539 kb on + strand at 606.540 kb on - strand at 606.573 kb on - strand at 606.573 kb on - strand at 606.573 kb on - strand at 606.620 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rhamnogalacturonan from soy bean pectic fibre 2.5 mg/ml (C) remove 603,999 + -1.4 604,059 + -0.5 604,314 + HMPREF1058_RS16755 0.25 +0.3 604,314 + HMPREF1058_RS16755 0.25 -1.8 604,436 + HMPREF1058_RS16755 0.42 -1.2 604,521 + HMPREF1058_RS16755 0.53 -1.9 604,658 + HMPREF1058_RS16755 0.72 -2.6 604,660 + HMPREF1058_RS16755 0.72 -3.3 605,066 + HMPREF1058_RS16750 0.24 -0.2 605,066 + HMPREF1058_RS16750 0.24 -1.6 605,071 + HMPREF1058_RS16750 0.25 +1.6 605,180 + -0.6 605,398 - +0.0 605,427 + HMPREF1058_RS16750 0.73 -1.5 605,428 - HMPREF1058_RS16750 0.73 -3.3 605,431 + HMPREF1058_RS16750 0.74 +0.0 605,432 - -1.5 605,611 - -2.6 605,612 + -0.1 605,612 + -0.2 605,687 - +1.2 605,688 + -0.8 605,689 - +0.0 605,689 - -0.3 605,689 - +1.4 605,712 - -0.1 605,776 + HMPREF1058_RS16745 0.15 +1.6 605,779 - HMPREF1058_RS16745 0.15 +2.0 605,789 + HMPREF1058_RS16745 0.16 +0.1 605,800 - HMPREF1058_RS16745 0.18 +1.2 605,815 + HMPREF1058_RS16745 0.19 +0.4 605,851 + HMPREF1058_RS16745 0.23 +1.7 605,851 + HMPREF1058_RS16745 0.23 -0.4 605,855 + HMPREF1058_RS16745 0.23 +0.6 605,872 - HMPREF1058_RS16745 0.25 +0.4 605,885 + HMPREF1058_RS16745 0.26 -0.0 605,885 + HMPREF1058_RS16745 0.26 +0.5 605,886 - HMPREF1058_RS16745 0.27 -0.8 605,889 - HMPREF1058_RS16745 0.27 +0.4 605,926 + HMPREF1058_RS16745 0.31 -1.1 605,929 - HMPREF1058_RS16745 0.31 -0.9 605,932 - HMPREF1058_RS16745 0.31 +1.0 605,970 + HMPREF1058_RS16745 0.35 +0.2 605,979 + HMPREF1058_RS16745 0.36 -0.8 606,013 - HMPREF1058_RS16745 0.40 +1.0 606,141 + HMPREF1058_RS16745 0.53 -0.7 606,142 - HMPREF1058_RS16745 0.54 -0.4 606,173 - HMPREF1058_RS16745 0.57 +0.7 606,235 + HMPREF1058_RS16745 0.63 +0.2 606,235 + HMPREF1058_RS16745 0.63 -0.0 606,425 + HMPREF1058_RS16745 0.83 -0.2 606,425 + HMPREF1058_RS16745 0.83 -0.6 606,426 - HMPREF1058_RS16745 0.83 +2.6 606,484 - HMPREF1058_RS16745 0.89 -1.4 606,518 + -0.0 606,519 - -1.9 606,539 + -0.2 606,540 - +0.6 606,573 - -0.6 606,573 - -0.2 606,573 - -2.6 606,620 + +1.1
Or see this region's nucleotide sequence