Experiment: Galactan from Potato 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS20915 and HMPREF1058_RS05835 are separated by 213 nucleotides HMPREF1058_RS05835 and HMPREF1058_RS05830 are separated by 21 nucleotides HMPREF1058_RS05830 and HMPREF1058_RS05825 are separated by 56 nucleotides HMPREF1058_RS05825 and HMPREF1058_RS05820 overlap by 14 nucleotides HMPREF1058_RS05820 and HMPREF1058_RS05815 are separated by 119 nucleotides
HMPREF1058_RS20915: HMPREF1058_RS20915 - HAD-IA family hydrolase, at 67,452 to 69,521
_RS20915
HMPREF1058_RS05835: HMPREF1058_RS05835 - 50S ribosomal protein L21, at 69,735 to 70,052
_RS05835
HMPREF1058_RS05830: HMPREF1058_RS05830 - 50S ribosomal protein L27, at 70,074 to 70,337
_RS05830
HMPREF1058_RS05825: HMPREF1058_RS05825 - thiamine diphosphokinase, at 70,394 to 71,029
_RS05825
HMPREF1058_RS05820: HMPREF1058_RS05820 - nicotinamide mononucleotide transporter, at 71,016 to 71,588
_RS05820
HMPREF1058_RS05815: HMPREF1058_RS05815 - serine--tRNA ligase, at 71,708 to 72,982
_RS05815
Position (kb) 70
71
72 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 69.487 kb on + strand at 69.498 kb on + strand at 69.498 kb on + strand at 69.498 kb on + strand at 69.498 kb on + strand at 69.498 kb on + strand at 69.499 kb on - strand at 69.499 kb on - strand at 69.503 kb on + strand at 69.504 kb on - strand at 69.505 kb on + strand at 69.505 kb on + strand at 69.505 kb on + strand at 69.519 kb on + strand at 69.519 kb on + strand at 69.542 kb on + strand at 69.542 kb on + strand at 69.543 kb on - strand at 69.543 kb on - strand at 69.546 kb on + strand at 69.548 kb on + strand at 69.549 kb on - strand at 69.575 kb on - strand at 70.327 kb on + strand at 70.395 kb on + strand at 70.523 kb on + strand, within HMPREF1058_RS05825 at 70.535 kb on - strand, within HMPREF1058_RS05825 at 70.535 kb on - strand, within HMPREF1058_RS05825 at 70.570 kb on - strand, within HMPREF1058_RS05825 at 70.582 kb on + strand, within HMPREF1058_RS05825 at 70.583 kb on - strand, within HMPREF1058_RS05825 at 70.583 kb on - strand, within HMPREF1058_RS05825 at 70.614 kb on + strand, within HMPREF1058_RS05825 at 70.627 kb on + strand, within HMPREF1058_RS05825 at 70.648 kb on + strand, within HMPREF1058_RS05825 at 70.650 kb on + strand, within HMPREF1058_RS05825 at 70.651 kb on - strand, within HMPREF1058_RS05825 at 70.681 kb on + strand, within HMPREF1058_RS05825 at 70.681 kb on + strand, within HMPREF1058_RS05825 at 70.714 kb on + strand, within HMPREF1058_RS05825 at 70.718 kb on - strand, within HMPREF1058_RS05825 at 70.721 kb on - strand, within HMPREF1058_RS05825 at 70.721 kb on - strand, within HMPREF1058_RS05825 at 70.917 kb on + strand, within HMPREF1058_RS05825 at 71.007 kb on - strand at 71.044 kb on - strand at 71.185 kb on + strand, within HMPREF1058_RS05820 at 71.189 kb on + strand, within HMPREF1058_RS05820 at 71.189 kb on + strand, within HMPREF1058_RS05820 at 71.259 kb on - strand, within HMPREF1058_RS05820 at 71.405 kb on - strand, within HMPREF1058_RS05820 at 71.477 kb on + strand, within HMPREF1058_RS05820 at 71.480 kb on - strand, within HMPREF1058_RS05820 at 71.530 kb on + strand, within HMPREF1058_RS05820 at 71.582 kb on - strand at 71.595 kb on + strand at 71.633 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Galactan from Potato 2.5 mg/ml (C) remove 69,487 + +0.1 69,498 + -0.2 69,498 + -0.6 69,498 + +1.0 69,498 + -1.3 69,498 + +0.5 69,499 - +2.2 69,499 - -1.0 69,503 + +0.8 69,504 - -1.1 69,505 + -1.3 69,505 + -0.2 69,505 + +0.8 69,519 + +1.6 69,519 + +0.2 69,542 + -0.8 69,542 + -0.1 69,543 - +0.8 69,543 - +0.0 69,546 + -1.2 69,548 + +1.6 69,549 - +0.6 69,575 - +0.1 70,327 + -0.3 70,395 + +0.0 70,523 + HMPREF1058_RS05825 0.20 -1.4 70,535 - HMPREF1058_RS05825 0.22 +0.7 70,535 - HMPREF1058_RS05825 0.22 +0.2 70,570 - HMPREF1058_RS05825 0.28 +0.9 70,582 + HMPREF1058_RS05825 0.30 +0.9 70,583 - HMPREF1058_RS05825 0.30 +0.6 70,583 - HMPREF1058_RS05825 0.30 +1.1 70,614 + HMPREF1058_RS05825 0.35 +1.8 70,627 + HMPREF1058_RS05825 0.37 +0.3 70,648 + HMPREF1058_RS05825 0.40 +0.6 70,650 + HMPREF1058_RS05825 0.40 -1.2 70,651 - HMPREF1058_RS05825 0.40 +2.8 70,681 + HMPREF1058_RS05825 0.45 -0.5 70,681 + HMPREF1058_RS05825 0.45 -0.2 70,714 + HMPREF1058_RS05825 0.50 -3.9 70,718 - HMPREF1058_RS05825 0.51 +0.8 70,721 - HMPREF1058_RS05825 0.51 +1.0 70,721 - HMPREF1058_RS05825 0.51 +0.4 70,917 + HMPREF1058_RS05825 0.82 +0.1 71,007 - +0.5 71,044 - +1.8 71,185 + HMPREF1058_RS05820 0.29 -0.4 71,189 + HMPREF1058_RS05820 0.30 -0.9 71,189 + HMPREF1058_RS05820 0.30 +0.3 71,259 - HMPREF1058_RS05820 0.42 +0.0 71,405 - HMPREF1058_RS05820 0.68 -0.5 71,477 + HMPREF1058_RS05820 0.80 -0.2 71,480 - HMPREF1058_RS05820 0.81 -0.8 71,530 + HMPREF1058_RS05820 0.90 -0.0 71,582 - -2.0 71,595 + -0.4 71,633 + -0.1
Or see this region's nucleotide sequence