Experiment: Galactan from Potato 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS04390 and HMPREF1058_RS04385 are separated by 174 nucleotides HMPREF1058_RS04385 and HMPREF1058_RS04380 are separated by 93 nucleotides HMPREF1058_RS04380 and HMPREF1058_RS04375 are separated by 114 nucleotides
HMPREF1058_RS04390: HMPREF1058_RS04390 - aspartate--tRNA ligase, at 45,709 to 47,472
_RS04390
HMPREF1058_RS04385: HMPREF1058_RS04385 - methylated-DNA--[protein]-cysteine S-methyltransferase, at 47,647 to 48,174
_RS04385
HMPREF1058_RS04380: HMPREF1058_RS04380 - DUF4251 domain-containing protein, at 48,268 to 48,858
_RS04380
HMPREF1058_RS04375: HMPREF1058_RS04375 - carbon-nitrogen hydrolase, at 48,973 to 49,860
_RS04375
Position (kb)
48
49 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 47.456 kb on + strand at 47.488 kb on + strand at 47.497 kb on + strand at 47.498 kb on - strand at 47.498 kb on - strand at 47.505 kb on - strand at 47.616 kb on + strand at 47.616 kb on + strand at 47.617 kb on - strand at 47.635 kb on + strand at 47.681 kb on - strand at 47.817 kb on + strand, within HMPREF1058_RS04385 at 47.843 kb on + strand, within HMPREF1058_RS04385 at 47.860 kb on + strand, within HMPREF1058_RS04385 at 47.969 kb on + strand, within HMPREF1058_RS04385 at 47.969 kb on + strand, within HMPREF1058_RS04385 at 47.970 kb on - strand, within HMPREF1058_RS04385 at 47.975 kb on - strand, within HMPREF1058_RS04385 at 48.170 kb on + strand at 48.170 kb on + strand at 48.199 kb on + strand at 48.200 kb on - strand at 48.202 kb on - strand at 48.207 kb on + strand at 48.254 kb on - strand at 48.261 kb on + strand at 48.270 kb on - strand at 48.290 kb on - strand at 48.294 kb on + strand at 48.294 kb on + strand at 48.336 kb on + strand, within HMPREF1058_RS04380 at 48.359 kb on - strand, within HMPREF1058_RS04380 at 48.361 kb on - strand, within HMPREF1058_RS04380 at 48.361 kb on - strand, within HMPREF1058_RS04380 at 48.361 kb on - strand, within HMPREF1058_RS04380 at 48.364 kb on - strand, within HMPREF1058_RS04380 at 48.441 kb on + strand, within HMPREF1058_RS04380 at 48.442 kb on - strand, within HMPREF1058_RS04380 at 48.443 kb on + strand, within HMPREF1058_RS04380 at 48.479 kb on - strand, within HMPREF1058_RS04380 at 48.479 kb on - strand, within HMPREF1058_RS04380 at 48.535 kb on - strand, within HMPREF1058_RS04380 at 48.594 kb on - strand, within HMPREF1058_RS04380 at 48.638 kb on - strand, within HMPREF1058_RS04380 at 48.638 kb on - strand, within HMPREF1058_RS04380 at 48.677 kb on - strand, within HMPREF1058_RS04380 at 48.685 kb on - strand, within HMPREF1058_RS04380 at 48.685 kb on - strand, within HMPREF1058_RS04380 at 48.699 kb on - strand, within HMPREF1058_RS04380 at 48.700 kb on - strand, within HMPREF1058_RS04380 at 48.827 kb on - strand at 48.874 kb on + strand at 48.885 kb on + strand at 48.886 kb on - strand at 48.909 kb on - strand at 48.970 kb on + strand at 49.086 kb on + strand, within HMPREF1058_RS04375 at 49.121 kb on - strand, within HMPREF1058_RS04375 at 49.276 kb on + strand, within HMPREF1058_RS04375 at 49.322 kb on - strand, within HMPREF1058_RS04375 at 49.322 kb on - strand, within HMPREF1058_RS04375 at 49.324 kb on - strand, within HMPREF1058_RS04375 at 49.330 kb on - strand, within HMPREF1058_RS04375 at 49.335 kb on - strand, within HMPREF1058_RS04375 at 49.335 kb on - strand, within HMPREF1058_RS04375 at 49.337 kb on - strand, within HMPREF1058_RS04375 at 49.337 kb on - strand, within HMPREF1058_RS04375 at 49.337 kb on - strand, within HMPREF1058_RS04375 at 49.338 kb on + strand, within HMPREF1058_RS04375 at 49.408 kb on + strand, within HMPREF1058_RS04375 at 49.408 kb on + strand, within HMPREF1058_RS04375 at 49.409 kb on - strand, within HMPREF1058_RS04375 at 49.503 kb on + strand, within HMPREF1058_RS04375 at 49.528 kb on + strand, within HMPREF1058_RS04375 at 49.610 kb on - strand, within HMPREF1058_RS04375 at 49.775 kb on - strand at 49.833 kb on + strand at 49.834 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Galactan from Potato 2.5 mg/ml (C) remove 47,456 + +1.5 47,488 + +2.1 47,497 + +1.0 47,498 - -0.1 47,498 - +0.4 47,505 - -0.8 47,616 + +1.0 47,616 + -0.4 47,617 - +0.6 47,635 + +0.1 47,681 - +0.1 47,817 + HMPREF1058_RS04385 0.32 +1.2 47,843 + HMPREF1058_RS04385 0.37 -0.8 47,860 + HMPREF1058_RS04385 0.40 +0.0 47,969 + HMPREF1058_RS04385 0.61 -0.0 47,969 + HMPREF1058_RS04385 0.61 -0.3 47,970 - HMPREF1058_RS04385 0.61 +3.0 47,975 - HMPREF1058_RS04385 0.62 -2.4 48,170 + +1.8 48,170 + -0.9 48,199 + +0.2 48,200 - -0.6 48,202 - +1.2 48,207 + +1.5 48,254 - +1.2 48,261 + +1.4 48,270 - -0.6 48,290 - -0.6 48,294 + -0.8 48,294 + +0.1 48,336 + HMPREF1058_RS04380 0.12 -1.8 48,359 - HMPREF1058_RS04380 0.15 +0.2 48,361 - HMPREF1058_RS04380 0.16 -1.7 48,361 - HMPREF1058_RS04380 0.16 +0.3 48,361 - HMPREF1058_RS04380 0.16 -0.5 48,364 - HMPREF1058_RS04380 0.16 -1.6 48,441 + HMPREF1058_RS04380 0.29 +0.2 48,442 - HMPREF1058_RS04380 0.29 +1.6 48,443 + HMPREF1058_RS04380 0.30 -0.1 48,479 - HMPREF1058_RS04380 0.36 -0.1 48,479 - HMPREF1058_RS04380 0.36 +0.3 48,535 - HMPREF1058_RS04380 0.45 +1.8 48,594 - HMPREF1058_RS04380 0.55 +0.3 48,638 - HMPREF1058_RS04380 0.63 +1.3 48,638 - HMPREF1058_RS04380 0.63 +0.1 48,677 - HMPREF1058_RS04380 0.69 +1.1 48,685 - HMPREF1058_RS04380 0.71 +1.8 48,685 - HMPREF1058_RS04380 0.71 -0.1 48,699 - HMPREF1058_RS04380 0.73 +1.4 48,700 - HMPREF1058_RS04380 0.73 +0.4 48,827 - +0.6 48,874 + +0.7 48,885 + -0.0 48,886 - +0.4 48,909 - +0.5 48,970 + -1.2 49,086 + HMPREF1058_RS04375 0.13 -1.5 49,121 - HMPREF1058_RS04375 0.17 -2.2 49,276 + HMPREF1058_RS04375 0.34 +0.2 49,322 - HMPREF1058_RS04375 0.39 -1.4 49,322 - HMPREF1058_RS04375 0.39 -3.4 49,324 - HMPREF1058_RS04375 0.40 -2.9 49,330 - HMPREF1058_RS04375 0.40 -1.8 49,335 - HMPREF1058_RS04375 0.41 -1.2 49,335 - HMPREF1058_RS04375 0.41 -0.0 49,337 - HMPREF1058_RS04375 0.41 -3.2 49,337 - HMPREF1058_RS04375 0.41 -2.3 49,337 - HMPREF1058_RS04375 0.41 -1.3 49,338 + HMPREF1058_RS04375 0.41 +0.1 49,408 + HMPREF1058_RS04375 0.49 -0.6 49,408 + HMPREF1058_RS04375 0.49 -0.5 49,409 - HMPREF1058_RS04375 0.49 -1.0 49,503 + HMPREF1058_RS04375 0.60 -0.8 49,528 + HMPREF1058_RS04375 0.62 -2.1 49,610 - HMPREF1058_RS04375 0.72 -0.6 49,775 - -0.4 49,833 + -1.7 49,834 - -0.0
Or see this region's nucleotide sequence