Experiment: Galactan from Potato 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS04120 and HMPREF1058_RS04115 are separated by 21 nucleotides HMPREF1058_RS04115 and HMPREF1058_RS04110 are separated by 104 nucleotides HMPREF1058_RS04110 and HMPREF1058_RS04105 are separated by 20 nucleotides HMPREF1058_RS04105 and HMPREF1058_RS21075 are separated by 9 nucleotides HMPREF1058_RS21075 and HMPREF1058_RS04100 are separated by 173 nucleotides
HMPREF1058_RS04120: HMPREF1058_RS04120 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 113,195 to 114,214
_RS04120
HMPREF1058_RS04115: HMPREF1058_RS04115 - CinA family protein, at 114,236 to 114,736
_RS04115
HMPREF1058_RS04110: HMPREF1058_RS04110 - 50S ribosomal protein L28, at 114,841 to 115,101
_RS04110
HMPREF1058_RS04105: HMPREF1058_RS04105 - 50S ribosomal protein L33, at 115,122 to 115,310
_RS04105
HMPREF1058_RS21075: HMPREF1058_RS21075 - DUF4295 domain-containing protein, at 115,320 to 115,475
_RS21075
HMPREF1058_RS04100: HMPREF1058_RS04100 - signal recognition particle-docking protein FtsY, at 115,649 to 116,608
_RS04100
Position (kb)
114
115 Strain fitness (log2 ratio)
-1
0
1
2
3 at 114.250 kb on + strand at 114.392 kb on - strand, within HMPREF1058_RS04115 at 114.448 kb on - strand, within HMPREF1058_RS04115 at 114.610 kb on + strand, within HMPREF1058_RS04115 at 114.625 kb on + strand, within HMPREF1058_RS04115 at 115.555 kb on - strand at 115.558 kb on - strand at 115.562 kb on + strand at 115.562 kb on + strand at 115.562 kb on + strand at 115.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Galactan from Potato 2.5 mg/ml (C) remove 114,250 + +2.6 114,392 - HMPREF1058_RS04115 0.31 +2.4 114,448 - HMPREF1058_RS04115 0.42 +0.4 114,610 + HMPREF1058_RS04115 0.75 +0.6 114,625 + HMPREF1058_RS04115 0.78 +3.2 115,555 - -1.0 115,558 - -0.5 115,562 + +0.2 115,562 + +0.2 115,562 + -0.0 115,610 + +2.8
Or see this region's nucleotide sequence