Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30135 and BPHYT_RS30140 are separated by 256 nucleotides BPHYT_RS30140 and BPHYT_RS30145 are separated by 110 nucleotides
BPHYT_RS30135: BPHYT_RS30135 - SAM-dependent methyltransferase, at 2,312,681 to 2,313,514
_RS30135
BPHYT_RS30140: BPHYT_RS30140 - 3-methylitaconate isomerase, at 2,313,771 to 2,314,958
_RS30140
BPHYT_RS30145: BPHYT_RS30145 - aconitate hydratase, at 2,315,069 to 2,317,666
_RS30145
Position (kb)
2313
2314
2315 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2313.109 kb on - strand, within BPHYT_RS30135 at 2313.356 kb on + strand, within BPHYT_RS30135 at 2313.597 kb on + strand at 2313.597 kb on + strand at 2313.606 kb on - strand at 2314.002 kb on - strand, within BPHYT_RS30140 at 2314.002 kb on - strand, within BPHYT_RS30140 at 2314.921 kb on + strand at 2314.921 kb on + strand at 2314.922 kb on - strand at 2314.922 kb on - strand at 2314.989 kb on - strand at 2315.488 kb on - strand, within BPHYT_RS30145 at 2315.844 kb on + strand, within BPHYT_RS30145 at 2315.844 kb on + strand, within BPHYT_RS30145 at 2315.845 kb on - strand, within BPHYT_RS30145 at 2315.845 kb on - strand, within BPHYT_RS30145 at 2315.922 kb on + strand, within BPHYT_RS30145
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB remove 2,313,109 - BPHYT_RS30135 0.51 +1.2 2,313,356 + BPHYT_RS30135 0.81 +0.9 2,313,597 + +1.5 2,313,597 + +0.1 2,313,606 - -0.9 2,314,002 - BPHYT_RS30140 0.19 -1.6 2,314,002 - BPHYT_RS30140 0.19 -3.3 2,314,921 + +1.0 2,314,921 + -1.8 2,314,922 - -0.0 2,314,922 - -0.3 2,314,989 - +0.4 2,315,488 - BPHYT_RS30145 0.16 -1.5 2,315,844 + BPHYT_RS30145 0.30 +0.1 2,315,844 + BPHYT_RS30145 0.30 +0.2 2,315,845 - BPHYT_RS30145 0.30 -4.4 2,315,845 - BPHYT_RS30145 0.30 -0.1 2,315,922 + BPHYT_RS30145 0.33 -1.9
Or see this region's nucleotide sequence