Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04245 and BPHYT_RS04250 are separated by 97 nucleotides BPHYT_RS04250 and BPHYT_RS04255 are separated by 49 nucleotides BPHYT_RS04255 and BPHYT_RS04260 are separated by 193 nucleotides
BPHYT_RS04245: BPHYT_RS04245 - dihydroorotase, at 972,638 to 973,915
_RS04245
BPHYT_RS04250: BPHYT_RS04250 - glycerol acyltransferase, at 974,013 to 974,858
_RS04250
BPHYT_RS04255: BPHYT_RS04255 - diadenosine tetraphosphatase, at 974,908 to 975,786
_RS04255
BPHYT_RS04260: BPHYT_RS04260 - GDP-6-deoxy-D-lyxo-4-hexulose reductase, at 975,980 to 976,891
_RS04260
Position (kb) 974
975
976 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 973.931 kb on + strand at 973.931 kb on + strand at 973.932 kb on - strand at 973.949 kb on + strand at 973.950 kb on - strand at 973.952 kb on - strand at 973.952 kb on - strand at 974.031 kb on - strand at 974.244 kb on + strand, within BPHYT_RS04250 at 974.244 kb on + strand, within BPHYT_RS04250 at 974.244 kb on + strand, within BPHYT_RS04250 at 974.244 kb on + strand, within BPHYT_RS04250 at 974.244 kb on + strand, within BPHYT_RS04250 at 974.244 kb on + strand, within BPHYT_RS04250 at 974.244 kb on + strand, within BPHYT_RS04250 at 974.244 kb on + strand, within BPHYT_RS04250 at 974.244 kb on + strand, within BPHYT_RS04250 at 974.245 kb on - strand, within BPHYT_RS04250 at 974.245 kb on - strand, within BPHYT_RS04250 at 974.245 kb on - strand, within BPHYT_RS04250 at 974.279 kb on - strand, within BPHYT_RS04250 at 974.538 kb on + strand, within BPHYT_RS04250 at 974.538 kb on + strand, within BPHYT_RS04250 at 974.538 kb on + strand, within BPHYT_RS04250 at 974.539 kb on - strand, within BPHYT_RS04250 at 974.539 kb on - strand, within BPHYT_RS04250 at 974.577 kb on + strand, within BPHYT_RS04250 at 974.577 kb on + strand, within BPHYT_RS04250 at 974.578 kb on - strand, within BPHYT_RS04250 at 974.578 kb on - strand, within BPHYT_RS04250 at 974.579 kb on + strand, within BPHYT_RS04250 at 974.645 kb on + strand, within BPHYT_RS04250 at 974.645 kb on + strand, within BPHYT_RS04250 at 974.646 kb on - strand, within BPHYT_RS04250 at 974.649 kb on + strand, within BPHYT_RS04250 at 974.649 kb on + strand, within BPHYT_RS04250 at 974.650 kb on - strand, within BPHYT_RS04250 at 974.650 kb on - strand, within BPHYT_RS04250 at 974.650 kb on - strand, within BPHYT_RS04250 at 974.650 kb on - strand, within BPHYT_RS04250 at 974.650 kb on - strand, within BPHYT_RS04250 at 974.868 kb on + strand at 974.868 kb on + strand at 974.868 kb on + strand at 974.909 kb on + strand at 974.909 kb on + strand at 974.909 kb on + strand at 974.909 kb on - strand at 974.910 kb on - strand at 974.910 kb on - strand at 974.910 kb on - strand at 975.477 kb on + strand, within BPHYT_RS04255 at 975.771 kb on + strand at 975.772 kb on - strand at 975.772 kb on - strand at 975.772 kb on - strand at 975.809 kb on + strand at 975.809 kb on + strand at 975.857 kb on + strand at 975.867 kb on + strand at 975.867 kb on + strand at 975.867 kb on + strand at 975.868 kb on - strand at 975.868 kb on - strand at 975.868 kb on - strand at 976.006 kb on + strand at 976.013 kb on - strand at 976.196 kb on + strand, within BPHYT_RS04260 at 976.197 kb on - strand, within BPHYT_RS04260 at 976.228 kb on - strand, within BPHYT_RS04260 at 976.353 kb on - strand, within BPHYT_RS04260 at 976.496 kb on + strand, within BPHYT_RS04260 at 976.497 kb on - strand, within BPHYT_RS04260 at 976.498 kb on + strand, within BPHYT_RS04260 at 976.499 kb on - strand, within BPHYT_RS04260 at 976.619 kb on + strand, within BPHYT_RS04260 at 976.634 kb on + strand, within BPHYT_RS04260 at 976.634 kb on + strand, within BPHYT_RS04260 at 976.635 kb on - strand, within BPHYT_RS04260
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB remove 973,931 + +1.2 973,931 + +0.3 973,932 - +1.5 973,949 + +0.4 973,950 - -3.1 973,952 - +0.2 973,952 - -0.4 974,031 - +0.4 974,244 + BPHYT_RS04250 0.27 -0.9 974,244 + BPHYT_RS04250 0.27 +0.4 974,244 + BPHYT_RS04250 0.27 -0.0 974,244 + BPHYT_RS04250 0.27 -1.6 974,244 + BPHYT_RS04250 0.27 -0.2 974,244 + BPHYT_RS04250 0.27 -0.1 974,244 + BPHYT_RS04250 0.27 +1.1 974,244 + BPHYT_RS04250 0.27 -2.5 974,244 + BPHYT_RS04250 0.27 +0.1 974,245 - BPHYT_RS04250 0.27 +0.2 974,245 - BPHYT_RS04250 0.27 +0.0 974,245 - BPHYT_RS04250 0.27 -0.3 974,279 - BPHYT_RS04250 0.31 -0.8 974,538 + BPHYT_RS04250 0.62 +0.0 974,538 + BPHYT_RS04250 0.62 -0.2 974,538 + BPHYT_RS04250 0.62 -0.6 974,539 - BPHYT_RS04250 0.62 -1.8 974,539 - BPHYT_RS04250 0.62 -0.5 974,577 + BPHYT_RS04250 0.67 +0.5 974,577 + BPHYT_RS04250 0.67 +0.4 974,578 - BPHYT_RS04250 0.67 +0.4 974,578 - BPHYT_RS04250 0.67 -2.7 974,579 + BPHYT_RS04250 0.67 -1.3 974,645 + BPHYT_RS04250 0.75 -0.6 974,645 + BPHYT_RS04250 0.75 -0.3 974,646 - BPHYT_RS04250 0.75 -0.8 974,649 + BPHYT_RS04250 0.75 +0.9 974,649 + BPHYT_RS04250 0.75 +0.8 974,650 - BPHYT_RS04250 0.75 -0.2 974,650 - BPHYT_RS04250 0.75 +0.2 974,650 - BPHYT_RS04250 0.75 -0.3 974,650 - BPHYT_RS04250 0.75 -0.6 974,650 - BPHYT_RS04250 0.75 -0.5 974,868 + -0.7 974,868 + -1.7 974,868 + +0.9 974,909 + -0.2 974,909 + -0.5 974,909 + -0.9 974,909 - -0.1 974,910 - +0.4 974,910 - -0.2 974,910 - +1.5 975,477 + BPHYT_RS04255 0.65 -1.8 975,771 + -0.5 975,772 - +0.8 975,772 - +1.0 975,772 - +0.2 975,809 + -1.2 975,809 + -0.5 975,857 + -0.4 975,867 + +0.9 975,867 + -2.0 975,867 + +0.2 975,868 - -0.1 975,868 - +0.1 975,868 - +0.8 976,006 + -0.6 976,013 - +1.7 976,196 + BPHYT_RS04260 0.24 +1.6 976,197 - BPHYT_RS04260 0.24 +0.1 976,228 - BPHYT_RS04260 0.27 +0.1 976,353 - BPHYT_RS04260 0.41 +1.5 976,496 + BPHYT_RS04260 0.57 +0.5 976,497 - BPHYT_RS04260 0.57 +0.2 976,498 + BPHYT_RS04260 0.57 +0.2 976,499 - BPHYT_RS04260 0.57 -2.6 976,619 + BPHYT_RS04260 0.70 -2.9 976,634 + BPHYT_RS04260 0.72 -1.2 976,634 + BPHYT_RS04260 0.72 +1.0 976,635 - BPHYT_RS04260 0.72 -0.3
Or see this region's nucleotide sequence