Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0656 and SMa0657 are separated by 150 nucleotides SMa0657 and SMa0659 are separated by 25 nucleotides SMa0659 and SMa0661 are separated by 366 nucleotides SMa0661 and SMa0662 are separated by 57 nucleotides
SMa0656: SMa0656 - TRm24 transposase, at 348,248 to 349,249
SMa0656
SMa0657: SMa0657 - cytochrome c binding protein, amino terminus, at 349,400 to 349,597
SMa0657
SMa0659: SMa0659 - cytochrome c binding protein, carboxyl terminus, at 349,623 to 350,021
SMa0659
SMa0661: SMa0661 - hypothetical protein, at 350,388 to 350,867
SMa0661
SMa0662: SMa0662 - transcriptional regulator FixK, at 350,925 to 351,560
SMa0662
Position (kb) 349
350
351 Strain fitness (log2 ratio) -2
-1
0
1
2 at 349.515 kb on + strand, within SMa0657 at 349.515 kb on + strand, within SMa0657 at 349.515 kb on + strand, within SMa0657 at 349.516 kb on - strand, within SMa0657 at 349.516 kb on - strand, within SMa0657 at 349.516 kb on - strand, within SMa0657 at 349.516 kb on - strand, within SMa0657 at 349.517 kb on + strand, within SMa0657 at 349.518 kb on - strand, within SMa0657 at 349.528 kb on + strand, within SMa0657 at 349.821 kb on - strand, within SMa0659 at 349.847 kb on - strand, within SMa0659 at 350.219 kb on + strand at 350.329 kb on - strand at 350.339 kb on + strand at 350.340 kb on - strand at 350.362 kb on + strand at 350.364 kb on + strand at 350.493 kb on + strand, within SMa0661 at 350.494 kb on - strand, within SMa0661 at 350.527 kb on + strand, within SMa0661 at 350.566 kb on + strand, within SMa0661
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 349,515 + SMa0657 0.58 +0.8 349,515 + SMa0657 0.58 -1.2 349,515 + SMa0657 0.58 +0.4 349,516 - SMa0657 0.59 +1.2 349,516 - SMa0657 0.59 +0.1 349,516 - SMa0657 0.59 -0.9 349,516 - SMa0657 0.59 -0.3 349,517 + SMa0657 0.59 -0.6 349,518 - SMa0657 0.60 +0.6 349,528 + SMa0657 0.65 +0.6 349,821 - SMa0659 0.50 +0.4 349,847 - SMa0659 0.56 +1.9 350,219 + -2.2 350,329 - +0.4 350,339 + -0.5 350,340 - +0.3 350,362 + -2.0 350,364 + +0.2 350,493 + SMa0661 0.22 +0.3 350,494 - SMa0661 0.22 +0.6 350,527 + SMa0661 0.29 +0.3 350,566 + SMa0661 0.37 -2.4
Or see this region's nucleotide sequence